Phytochemical and biological studies of <i>Atriplex inflata</i> f. Muell.: isolation of secondary bioactive metabolites

Nejma, Aymen Ben; Znati, Mansour; Nguir, Asma; Daı̈ch, Adam; Othman, Mohamed Rozali; Lawson, Ata Martin; Jannet, Hichem Ben

doi:10.1111/jphp.12735

Cited by 14 publications

(9 citation statements)

References 28 publications

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“…Collectively, most of these phenolic compounds were detected in other Atriplex spp. (e.g., aerial parts of A. halimus ), but a direct qualitative comparison is difficult because of the different extraction conditions reported in the literature [ 23 , 24 , 25 , 26 ].…”

Section: Resultsmentioning

confidence: 99%

Atriplex mollis Desf. Aerial Parts: Extraction Procedures, Secondary Metabolites and Color Analysis

et al. 2018

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A method using high-performance liquid chromatography coupled with a photodiode array detector was proposed for the rapid characterization of different phenolic constituents from the extracts of Atriplex mollis aerial parts. Atriplex species are known for their multiple biological activities, but no information is available in the literature about A. mollis. With the aim to firstly characterize the main secondary metabolites of this plant, so as to orient better the biological evaluation, we applied three different extraction procedures and compared the chromatographic results. Microwave-assisted extraction gave the best yield and recovery of important compounds such as gallic acid, catechin, chlorogenic acid, p-OH benzoic acid, rutin, sinapinic acid, t-ferulic acid, naringenin and benzoic acid. These constituents belong to three important chemical classes: phenolic acids, flavonoids and monoterpenes. Color evaluation and analysis of chlorophylls (a and b) and carotenoids complete the preliminary profile of this plant. From these analyses, Atriplex mollis is a source of bioactive compounds (especially rutin, t-ferulic acid and gallic acid) and could be recommended as a plant of phyto-pharmaceutical relevance, opening new perspectives on this salt-tolerant plant.

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Section: Resultsmentioning

confidence: 99%

Atriplex mollis Desf. Aerial Parts: Extraction Procedures, Secondary Metabolites and Color Analysis

et al. 2018

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“…The 2,2 ′ -azino-bis-3-ethylbenzotiazolin-6-sulfonic acid (ABTS) scavenging assay is based on radical cation decolorization method. In this method, reduction of ABTS •+ radicals depend on plant extracts antioxidant capacity (electron donating capacity) which leads to decolorization of the radical (Ben et al, 2017). Two solutions, ABTS (7 mM) and K 2 S 2 O 8 (2.45 mM) was prepared in deionized water and were mixed in 1:1 ratio followed by dark incubation for 24-48 h. Afterward, dilution of ABTS solution was prepared in 1:25 ratio and 300 µl of plant extract was added to 3.0 ml of ABTS •+ solution.…”

Section: Abts Scavenging Assaymentioning

confidence: 99%

Phytochemical Characterization and Assessment of Crude Extracts From Lantana camara L. for Antioxidant and Antimicrobial Activity

et al. 2020

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“…The AcOEt extracts, BuOH extracts and the TEBN were tested in terms of radical scavenging ability using stable DPPH radical according to the method described by Henry et al . and Ben Nejma et al . with slight modifications.…”

Section: Methodsmentioning

confidence: 99%

“…The radical scavenging capacity of the AcOEt extracts, BuOH extracts and TEBN on the ABTS (2.2′‐azinobis‐3‐ethylbenzothiazoline‐6‐sulfonic acid) radical was assayed as described by Re et al . and Ben Nejma et al . with minor modification.…”

Section: Methodsmentioning

confidence: 99%

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Characterization of Polar and Non‐Polar Compounds of House Edible Bird's Nest (EBN) from Johor, Malaysia

Lee

Azmi

et al. 2020

Chemistry & Biodiversity

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and Reactivity (LR11ES39), Team: Medicinal Chemistry and Natural Products, Avenue of Environment, 5019 Monastir, TunisiaThis work investigated the polar (PC: protein, amino acid and metabolite) and non-polar (NPC: fatty acid) compounds and bioactivity characteristics of the EBN harvested from the state of Johor in Malaysia. The electrophoretic gels exhibited 15 protein bands (16-173 kD) with unique protein profile. Amino acids analysis by AccQ·Tag method revealed 18 types of amino acids in EBN. Metabolite profiling was performed using High-Performance Liquid Chromatography coupled with Quadrupole Time-of-Flight Mass Spectrometer (HPLC-QTOF/ MS) technique and a total of 54 compounds belonging to different groups were detected and identified. These findings help to uncover the relation of therapeutic activity of EBN. The EBN was further extracted with AcOEt and BuOH. The AcOEt extract was fractionated into three fractions (F 1 À F 3 ), and the high triglyceride content in F 2 was verified by gC-FID. The three groups of fatty acids discovered in EBN are 48.43 % of poly-unsaturated (PUFA), 25.35 % of saturated fatty acids (SFA) and 24.74 % of mono-unsaturated fat (MUFA). This is the first time to report results ofEBN, BuOH, and AcOEt extracts and of fraction F 2 (TEBN) on their analysis for their antioxidant activities by DPPH, ABTS and catalase assay and for their paraoxonase and anti-tyrosinase activities. The results showed that TEBN exhibited the significant bioactivity in all assays. These findings suggest that TEBN is a good source for natural bioactive compounds in promoting body vigor. Current work widened the content of EBN especially on the triglyceride and also marked the content of specific location (Johor, Malaysia) of EBN origin.[a] IC 50 (mg/mL): the concentration at which 50 % is inhibited. [b] TEBN: Triglycerides of EBN. [c] BHT: butylated hydroxytoluene. [d] u/ mg protein: μmol of H 2 O 2 destroyed/min/mg protein. [e] IU: mM/min. Values were expressed as mean � SD (n = 3), Different letters in columns indicate significant differences among fractions at P < 0.05 (LSD test).

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Phytochemical and biological studies of Atriplex inflata f. Muell.: isolation of secondary bioactive metabolites

Abstract: In the results of the present work, we have proposed the biogenesis pathway of unsaturated fatty acid and described the structure-activity relationship.

Cited by 14 publications

References 28 publications

Atriplex mollis Desf. Aerial Parts: Extraction Procedures, Secondary Metabolites and Color Analysis

Atriplex mollis Desf. Aerial Parts: Extraction Procedures, Secondary Metabolites and Color Analysis

Phytochemical Characterization and Assessment of Crude Extracts From Lantana camara L. for Antioxidant and Antimicrobial Activity

Characterization of Polar and Non‐Polar Compounds of House Edible Bird's Nest (EBN) from Johor, Malaysia

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