Phytochemical Characterization, Anti-Oxidant, Anti-Enzymatic and Cytotoxic Effects of Artemisia verlotiorum Lamotte Extracts: A New Source of Bioactive Agents

Abstract

Artemisia verlotiorum Lamotte is recognized medicinally given its long-standing ethnopharmacological uses in different parts of the world. Nonetheless, the pharmacological properties of the leaves of the plant have been poorly studied by the scientific community. Hence, this study aimed to decipher the phytochemicals; quantify through HPLC-ESI-MS analysis the plant’s biosynthesis; and evaluate the antioxidant, anti-tyrosinase, amylase, glucosidase, cholinesterase, and cytotoxicity potential on normal (NIH 3T3) and human liver and human colon cancer (HepG2 and HT 29) cell lines of this plant species. The aqueous extract contained the highest content of phenolics and phenolic acid, methanol extracted the most flavonoid, and the most flavonol was extracted by ethyl acetate. The one-way ANOVA results demonstrated that all results obtained were statistically significant at p < 0.05. A total of 25 phytoconstituents were identified from the different extracts, with phenolic acids and flavonoids being the main metabolites. The highest antioxidant potential was recorded for the aqueous extract. The best anti-tyrosinase extract was the methanolic extract. The ethyl acetate extract of A. verlotiorum had the highest flavonol content and hence was most active against the cholinesterase enzymes. The ethyl acetate extract was the best α-glucosidase and α-amylase inhibitor. The samples of Artemisia verlotiorum Lamotte in both aqueous and methanolic extracts were found to be non-toxic after 48 h against NIH 3T3 cells. In HepG2 cells, the methanolic extract was nontoxic up to 125 µg/mL, and an IC50 value of 722.39 µg/mL was recorded. The IC50 value exhibited in methanolic extraction of A. verlotiorum was 792.91 µg/mL in HT29 cells. Methanolic extraction is capable of inducing cell cytotoxicity in human hepatocellular carcinoma without damaging normal cells. Hence, A. verlotiorum can be recommended for further evaluation of its phytochemical and medicinal properties.

Effects of Artemisia ordosica Crude Polysaccharide on Antioxidant and Immunity Response, Nutrient Digestibility, Rumen Fermentation, and Microbiota in Cashmere Goats

“…At present, studies on crude polysaccharides from Artemisia ordosica are mainly focused on mice, pigs, and poultry, and studies on goats have not been reported. Weaned piglets' apparent digestibility of nutrients and feed conversion efficiency were markedly improved by a water extract of Artemisia ordosica (750 mg/kg) in a dose-dependent manner, as well as considerably reducing their DMI from 14 to 28 days [10]. According to our study, AOCP improved the digestibility of DM, CP, and ADF, and increased ADG and feed conversion ratios in cashmere goats.…”

“…However, no application studies in sheep have been reported. Among the effective compounds of Artemisia ordosica crude polysaccharide, polysaccharides have better hydrophilicity and a variety of biological activities, such as enhancing cell-mediated antioxidant [10], anti-inflammation [11] and antitumor [12] activities. Broilers stimulated by LPS (100 g/kg) exhibit reduced immune function and production performance that is alleviated by AOCP [13].…”

Bioprospecting of Artemisia genus: from artemisinin to other potentially bioactive compounds

“…or the use of reference compounds other than Trolox. A few recent studies report the antioxidant activity of methanolic extracts of various Artemisia species in comparison to Trolox 19 , 28 , 63 (Supplementary Table 2 ). Our results are in line with the trend observed by Trifan and colleagues for FRAP assay, in which A. absinthium displayed the lowest antioxidant activity.…”

Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of Artemisia nilagirica (C.B. Clarke) Pamp

“…The Artemisia genus and the member species are well-studied for their various biological activities [ 8 , 9 , 10 , 11 , 12 , 13 ]. Artemisia annua L. has demonstrated significant medicinal benefits because of the presence of artemisinin [ 14 ].…”