Phytochemical evaluation of Plumbago zeylanica roots from Indonesia and assessment of its plumbagin concentration

Abstract

Introduction:Plumbago zeylanica grows widely in many tropical countries. In Indonesia, this plant, known as Daun Encok, has some beneficial effects on human health.  Aim: This exploration study aimed to identify the plumbagin compound in P. zeylanica roots from Indonesia.  Materials and methods: Dried roots of P. zeylanica were manually ground and then the powder was macerated using ethanol and chloroform for 24 hours at room temperature. All extracts of P. zeylanica were then analyzed using gas chromatography-mass spectrometry (GC-MS). Plumbagin concentration was measured by comparing the extract with pure plumbagin. Results: GC-MS analysis of ethanol extract and chloroform extract of P. zeylanica roots showed the presence of plumbagin as the highest peak. Plumbagin concentration in ethanol extract was 13%, while in chloroform extract it was 81%. Conclusions: The chloroform extract of P. zeylanica root from Indonesia demonstrates a higher concentration of plumbagin compared to ethanol extract. 

Chloroform Extract of Plumbago zeylanica Linn. Roots Ameliorates the Epidermal Thickness of Imiquimod-induced Psoriatic Mice through Cell Cycle and Apoptosis

“…Dried roots were then grounded into powder which macerated in chloroform (Merck) with 1:10 ratio for 24 h. After filtration with Whatman filter paper, the filtrate was concentrated using a rotary evaporator and was kept in a refrigerator until further use. The secondary metabolites contained in our extract were characterized using GC-MS technique [23].…”

In Silico Study of Plumbagin as Potent Inhibitor of Pro-Inflammatory Molecules TNF-, NF-, and IL-17

“…Plumbagin (5-hydroxy-2-methyl-1,4naphthoquinone or 5-hydroxy-2-methylnaphthalene-1,4-dione) is reportedly abundant in the roots of the Plumbago zeylanica L. plants. [5][6][7] Many in vitro and in vivo studies have used extracts from Plumbago zeylanica L. roots, such as in the rat model of carrageenan-induced paw edema, a rat model of thermal stimuli, and in vitro study of A549 lung cancer cells. [8][9][10] However, few studies have directly used the active compound Plumbagin.…”