Background:UV radiation induced ROS cause DNA damage mainly due to photoaging. Lipid peroxidation due to UV exposure also produces non-toxic metabolite compounds namely malonyldialdehyde (MDA). Upregulation of TNF-α is key to the initial response to ultraviolet B (UVB) exposure by keratinocytes. The basal cell layer is the most affected undergoes to apoptosis and can be detected through caspase 3 which is the main executor of apoptosis. Mangosteen peel (Garcinia mangostana Linn.)contains xanthon known as alpha mangosteen, the most attention today is as an antioxidant. The purpose of this study is to determine the antioxidant effects of ethyl acetate extract in mangosteen peel cream on MDA levels, anti-inflammatory effects on TNF-α levels and anti-apoptosis effects on caspase 3 levels, testedin guinea pig skin exposed to UVB rays. Methods :This study uses a post test only control group design using female guinea pig (Cavia porcellus) who obtained, cared, and treated at the Wates Veterinary Center, Yogyakarta. Consisting of 20 animals divided randomly into 4 treatment groups.P1: normal group, without any treatment. P2: exposed to UVB light. P3: exposed to UVB light and given base cream. P4: exposed to UVB light and given 12% extract mangosteen peel cream. MDA were analyzed by the TBARS method (nmol/ml). Levels of TNF-α epidermis were obtained by the TNF alpha Elisa antibodies Kit (pg/ml). Levels of caspase 3 epidermis were obtained by the caspase antibody 3 Elisa Kit (pmol / L). Results:Post Hoc statistical analysis showed that MDA serum levels of P4 group was significantly lower compared to P2 group(p<0.01), TNF-αepidermis levels of P4 groupwas significant lower compared to P2 group(p<0,001) and caspase 3 epidermis levels of P4 groupwas significant lower compared to P2 group (p<0.01). Conclusion:Administration of 12% dose ethyl acetate extract of mangosteen peel cream is proven to significantly lower MDA levels, TNF-α levels and Caspase-3 levels in the epidermal tissues of guinea pig skin exposed to ultraviolet B light. Bangladesh Journal of Medical Science Vol. 21 No. 03 July’22 Page: 512-520

Introduction:Plumbago zeylanica grows widely in many tropical countries. In Indonesia, this plant, known as Daun Encok, has some beneficial effects on human health.&nbsp; Aim: This exploration study aimed to identify the plumbagin compound in P. zeylanica roots from Indonesia.&nbsp; Materials and methods: Dried roots of P. zeylanica were manually ground and then the powder was macerated using ethanol and chloroform for 24 hours at room temperature. All extracts of P. zeylanica were then analyzed using gas chromatography-mass spectrometry (GC-MS). Plumbagin concentration was measured by comparing the extract with pure plumbagin. Results: GC-MS analysis of ethanol extract and chloroform extract of P. zeylanica roots showed the presence of plumbagin as the highest peak. Plumbagin concentration in ethanol extract was 13%, while in chloroform extract it was 81%. Conclusions: The chloroform extract of P. zeylanica root from Indonesia demonstrates a higher concentration of plumbagin compared to ethanol extract.&nbsp;

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