Phytochemical profile and in vitro antioxidant activity of Emelia M (EMB), Mshikazi and Delosma H herbal medicines as demonstrated in THP-1 and Jurkat leukaemia cell lines

Abstract

Background: Three decoctions, namely Emelia M (EMB), Mshikazi and Delosma H are used by traditional health practitioners in KwaZulu-Natal, South Africa to treat and manage leukaemia and related conditionsObjectives: This study evaluated the in vitro antioxidant activity and phytochemical profile of the aqueous extracts of Emelia M (EMB), Mshikazi and Delosma H decoctions.Methods: Antioxidant activity of the extracts was evaluated using1-diphenyl-2-picrylhydrazyl (DPPH), glutathione (GSH), phosphomolybdate and thiobarbituric acid reactive substance (TBARS) assays. Phytochemical screening was used to determinethe presence of compounds.Results: The DPPH radical scavenging activity was similar to ascorbic acid for EMB and Delosma H, but not for Mshikazi. At 24 h, EMB increased GSH in both THP-1 and Jurkat cells similar to Delosma H while Mshikazi demonstrated the lowest activity. At 48 h, EMB and Delosma H revealed increased GSH in THP-1 cells with no significant decrease in GSH levels in Jurkat cells. However, EMB showed the lowest lipid peroxidation activity compared to Delosma H and Mshikazi after 24 h treatment of both cells. Phenols, flavonoids, terpenoids, saponins were present in all extracts.Conclusion: Extracts of the three decoctions possess both antioxidant and prooxidant properties through high scavenging activity and increased in lipid peroxidation.
 Keywords: Antioxidants; herbal medicines; Emelia M; Mshikazi; Delosma H.

Phytochemical Screening, Antibacterial and Insecticidal Activities of Stem Bark Extracts of Boswellia Dalzielii Hutchin From Kaltungo, Nigeria

“…In studies previously conducted on plant extracts, it has, shown that the phytochemicals present in solvent extracts was known to have wide range of biological activates including antibacterial, antifungal, antiviral, antioxidant, cytotoxic, and insecticides properties (Ahmed et al,2022;Ricardo et al, 2022;Ashish et al, 2022;Adeniyi et al, 2021;Mshelia et al, 2008). This study was carried out in the same line in order to find out the phytochemicals present in the extracts obtained with different solvents and their biological activities such as antibacterial and insecticidal properties.…”

“…The activities of the acetone and the ethyl acetate that inhibited five of the test organisms each may also be due to the presence of the flavonoids which was also present in moderate amount in both the extracts (Zaria et al, 1995). Adeniyi et al, 2021;Khalid et al, 2018 reported that phytochemicals possesses antimicrobial and antioxidant properties. The presence of alkaloids and anthraquinones in the ethanol extract can also be a contributory factor for its high activity on the test organisms.…”

Probing New Antileukemia Agents That Target FLT3 and BCL-2 from Traditional Concoctions through a Combination of Mass Spectrometry Analysis and Consensus Docking Methods

“…This study considers the inhibitory activities of the proposed ligands from three samples of traditional concoctions that are named Emelia M, Mshikazi and Delosma H decoctions [18], against three leukemia targets BCL-2 (PDB 6o0k) and mutated BCL-2 G101V (PDB: 6o0l). To efficiently probe the inhibitory activities of the potential drug candidates, seven scoring functions and five consensus methods were used to combine the results.…”