Phytochemical Profile and Total Antioxidant Capacity of Sempervivum ruthenicum Koch Hydroethanolic Extract

Abstract

The scope of this work is a phytochemical analysis and antioxidant activity assay of a Sempervivum ruthenicum Koch hydroethanolic extract. The hydroethanolic extract was prepared from the dried leaves of the plant by maceration in a water and methanol mixture (50:50 v/v). The total phenolic content of the extract was calculated to be 3.0501�0.0272 mg/mL and the total flavonoid content was determined in a concentration of 3.113�0.0394 mg/mL. The HPLC-DAD analysis revealed phenolic acids and flavonoids, which were quantified. The most prevalent phenolic acids in the extracts were gallic and ellagic acids, with concentrations of 1.2443�0.0475 mg/mL, respectively 0.6339�0.0026 mg/mL. The heteroside astragalin was present in high concentration of 1.1934�0.0754 mg/mL. The DPPH free radical scavenging assay revealed the EC50 value of the extract to be 2.5788�0.003 mg/mL. These results suggest a good scavenging ability of the extract, which is due to the abundance of polyphenolic compounds. The antioxidant activity of the extract demonstrates a high scavenging ability at low doses. Sempervivum ruthenicum Koch shows a promising phytochemical profile that suggests it�s use in pathological conditions that involve high oxidative stress.

Semperivium Ruthenicum Koch Extract-Loaded Bio-Adhesive Formulation: A Novel Oral Antioxidant Delivery System for Oxidative Stress Reduction

“…It is worth noting, however, that the extract from fresh plant material had significantly lower concentrations of bioactive compounds compared to the dry plant extract. These results are consistent with previously published works [ 27 , 28 ] and indicate that the Sempervivum ruthenicum Koch dry plant extract is a rich source of various bioactive compounds, especially polyphenols and flavonoids, which may offer health benefits upon consumption.…”

A Comparative Analysis of the Phytochemical Profiles of <em>Lonicera japonica Thunb. Flos Thunb.</em> Species Native to the Mountainous and Coastal Regions of Romania

“…al. [34] as a quick and easy way to determine the TPC of a wide variety of materials, including plant extracts.…”

“…To accurately determine the total flavonoid content of a plant extract, the method described by Mihai et al [34] was employed. This method used quercetin as a reference compound, which allowed for the results to be expressed as quercetin equivalents present in the sample.…”

Studies on the Phytochemical Composition and Antioxidant Activity of a Prunus spinosa L. Aqueous Extract

“…The purple color of the DPPH solution at 517 nm changes to pale yellow due to the appearance of the reduced form of the free radical after contact with the sample solution containing active principles with free radical scavenging potential. The method used was decribed in a previous study [14], and the total scavenging ability of the extract was calculated with the following equation: % scavenger activity = 100 x (Ainitial -Afinal)/Ainitial, where: Ainitial = Absorbance before addition of the plant extract and Afinal = absorbance 15 minutes after addition of the solution to be analyzed. The IC50 (concentration of plant extract that scavenges 50% of the free radical) was calculated using the linear regression method and is expressed by constructing the dose-response curve [15].…”