Phytochemical Profile, Antioxidant and Wound Healing Potential of Three Artemisia Species: In Vitro and In Ovo Evaluation

Abstract

Skin injuries, and especially wounds of chronic nature, can cause a major negative impact on the quality of life. New efficient alternatives are needed for wound healing therapy and herbal products are being investigated due to a high content of natural compounds with promising healing activity. For this purpose, we investigated three Artemisia species, Artemisia absinthium L. (AAb), Artemisia dracunculus L. (ADr) and Artemisia annua L. (AAn). Ethanolic extracts, containing different polyphenolic compounds, elicited strong antioxidant activities in the DPPH assay, comparable to ascorbic acid. Human ketratinocyte proliferation was stimulated and wound closure was enhanced by all three extracts at concentrations of 100 µg/mL. The Artemisia extracts modulated angiogenesis by increasing vessel formation, especially following treatment with A. annua and A. dracunculus, extracts with a significantly higher content of chlorogenic acid. Good tolerability and anti-irritative effects were also registered in ovo, on the chorioallantoic membrane (CAM). The three Artemisia species represent promising low-cost, polyphenol-rich, antioxidant, safe alternatives for wound care treatment.

Artemisia abrotanum and Symphytum officinale Polyphenolic Compounds-Rich Extracts with Potential Application in Diabetes Management

“…The A. abrotanum polyphenolic compound-rich extract had higher quantities of rutin (10.57 µg/mL), umbelliferone (17.03 µg/mL), and chlorogenic acid (103.47 µg/mL), while S. officinale polyphenolic compound-rich extract had higher quantities of caffeic acid (9.42 µg/mL), ellagic acid (43.79 µg/mL), and rosmarinic acid (136.140 µg/mL). As shown in other studies, chlorogenic acid and rutin have also been identified as major constituents in Artemisia ethanolic extract from other species [ 40 , 41 ]. Although there are few studies on the polyphenolic compounds profile of S. officinale extracts, rosmarinic and caffeic acid were also found to be major polyphenols by Sowa et al [ 42 ], Trifan et al [ 43 ], and the presence of caffeic acid was also reported by Nastić et al [ 44 ].…”

Aqueous Extract of Artemisia annua Shows In Vitro Antimicrobial Activity and an In Vivo Chemopreventive Effect in a Small-Cell Lung Cancer Model

“…Interestingly, the data also suggested the bactericidal potential of AAE against MRSA, as a 21 mm zone of inhibition ( Figure 2 C and Table 1 ) was found, and more than 90% of the cells ( Figure 2 C) were dead when treated with 6 mg/mL. Earlier, some studies reported the efficacy of A. turanica and A. dracunculus against MRSA in vitro and in vivo respectively [ 51 , 52 ]. Previous studies suggested the antimicrobial potential of plant extracts against MRSA due to the presence of the compounds having β-lactamase inhibiting properties [ 53 , 54 ].…”

A comprehensive study on essential oil compositions, antioxidant, anticholinesterase and antityrosinase activities of three Iranian Artemisia species

“…Previous study on A. biennis Willd, showed that, the hydroethanolic extract of the plant had the highest amount of phenolic content and antioxidant activity 31 . Another research on A. absinthium demonstrated that the ethanolic extract had more TPC than A. dracunculus and A. annua 32 . In another study, among six Artemisia species, A. oliveriana had the highest TPC and A. diffusa had the highest TFC 33 .…”