Phytochemical profiling of petroleum ether and chloroform extracts of Curcuma caesia rhizome by GC-MS and comparing their bioactivities

Abstract

The objective of this study was to characterise the putative phytochemical constituents from petroleum ether (CC-P) and chloroform (CC-C) extracts of Curcuma. caesia rhizome by GC-MS analysis and to compare their bioactivities. GC-MS analysis facilitated the documentation of a total of 16 and 20 volatile constituents in CC-P and CC-C extracts respectively. Of these the major constituents present in CC-P were cycloisolongifolence, 8, 9-dehydro-9 formyl- (19.64%), 4-oxo- β-isodamascol (16.05%), 6,10-dimethyl-3- (1-methylethyl) -6-cyclodecene-1,4-dione (13.80%), 2H-cyclohepta[b]furan-2-one,6-[1-(acetyloxy) -3-oxobutyl]- 3,3a,4,7,8,8a- hexahydro-7-methyl- 3-methylene- (9.08%), tricycle [5.1.0.0(2,4)] oct-5-ene-5-propanoic acid,3,3,8.8-tetramethyl- (6.39%), 2(3H)-benzofuranone,6-ethenylhexahydro-3,6-dimethyl-7-(1- methyl ethenyl)-, [3S-(3α,3aα,6α,7β,7aβ) (4.18%) and 7a-isopropenyl-4,5-dimethy loctahydroindene-4-carboxylic acid (4.01%). Similarly, the major constituents present in CC-C were cycloisolongifolence,8,9- dehydro-9-formyl-(15.69%), 6,10-dimethyl-3-(1-methylethyl)-6-cyclodecene-1, 4-dione (13.38%), 2H-cyclohepta [b]furan-2-one,6-[1- (acetyloxy)-3-oxobutyl] -3,3a,4,7,8,8a- hexa hydro -7-methyl - 3-methylene- (4.55%), tricycle [5.1.0.0(2,4)] oct-5-ene-5- propanoic acid,3,3,8.8-tetramethyl- (7.50%), 2(3H)-benzofuranone, 6-ethenylhexahydro-3,6-dimethyl-7-(1-methylethenyl)-,[3S- (3α,3aα,6α,7β,7aβ) (6.15%), 7a-isopropenyl-4,5-dimethyl octahydroindene-4-carboxylic acid (6.43%) and 2-(4a,8- Dimethyl-6- oxo-1,2,3,4-4a,5,6,8a-octahydro-naphthalen-2-yl) propionaldehyde (6.57%). The extracts, CC-P and CC-C exhibited almost similar antibacterial and antifungal potentials but differed with respect to antioxidant activity. The IC50 values of CC-P and CC-C extracts for DPPH scavenging were 0.68±0.02 mg ml-1 and 0.13±0.01 mg ml-1 respectively. Together, above results provide an important basis for the isolation of bioactive compounds from C. caesia rhizome extract and their use as traditional medicine.

GC-MS Profiling, in vitro Antioxidant and Antimicrobial Activities of Kaempferia parviflora Wall. ex. Baker Rhizome Extract

“…The extract was examined for inhibition of lipid peroxidation by using soy lecithin liposomes and thiobarbituric acid (TBA) regent. 19,21 GC-MS Experiment GC-MS experiment was performed on a GC instrument (Agilent Technologies 7890 A) coupled to mass spectrometer (AccuTOF GCv, JMS-T100GCV, JEOL, Japan) under the following experimental conditions as reported previously. 19 About 1 μl of the sample was injected using helium as a carrier gas with a flow rate of 1 ml/min.…”

“…19,21 GC-MS Experiment GC-MS experiment was performed on a GC instrument (Agilent Technologies 7890 A) coupled to mass spectrometer (AccuTOF GCv, JMS-T100GCV, JEOL, Japan) under the following experimental conditions as reported previously. 19 About 1 μl of the sample was injected using helium as a carrier gas with a flow rate of 1 ml/min. The analytical column attached to the GC was HP5 column and the injector temperature was set at 250°C.…”

“…Qualitative screening of phytochemicals in the extract was performed using the standard protocol reported earlier. 19…”

“…The DPPH • / ABTS •radical scavenging assay. 19,21 against various pathogens might be due to the diffusing potency of the extract against the particular organism. These results thus suggested that the extract remarkably exhibited broad spectrum of activity, especially toward the bacteria.…”

“…Baker Rhizome Extract described previously. 19,21 The 50% inhibitory concentration (IC 50 ) value (concentration of the extract needed to reduce the absorbance of DPPH • / ABTS •radicals by 50%) was calculated from the percentage scavenging activity of extract.…”