Phytochemical Screening and Antioxidant Analysis of the Ethanolic Extract of Rosehip Seed Press Cake

Abstract

Aims: Rosehip seed press cake is a waste material in the industrial extraction of rosehip seed oil using cold press process. The current study seeks to evaluate the phytochemical profile, total phenolic content, total flavonoid content and DPPH radical scavenging activity of the ethanolic extract of rosehip seed press cake using standard methods and DPPH assay.
 Study Design: This is an experimental laboratory report on the phytochemical properties and antioxidant potential of the ethanolic semi-solid extract (ESE) of rosehip seed press cake in order to assess its commercial viability as a food supplement.
 Place and Duration of Study: The work was conducted in the Department of Chemistry, National University of Lesotho, from August 2019 to March 2020. 
 Methodology: Ethanol was used for the extraction of the semi-solid extract (ESE) from the rosehip seed press cake. The ESE was analysed for phytochemical constituents using standard methods. In vitro antioxidant activity of the ESE was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. For data analysis, mean and standard deviation were computed for each parameter that was quantitatively determined.
 Results: Presence of flavonoids, alkaloids, terpenoids, sterols, glycosides, reducing sugars, proteins, amino acids, fatty acids, phenols and polyphenols in the ESE was confirmed. Total phenolics and flavonoids content were found to be 134.44 mg GAE/g DW and 73.23 mg QE/g DW, in the range of 200 to 3000 µg/ml respectively. The DPPH radical scavenging activity of the extract was found to be in the range 10.32±3.89 and 76.06±3.48% within the concentration range and was very close to the scavenging activity of DPPH at concentrations ≥ 1500 µg/ml. The ESE showed an IC50 value of 1367.06 µg/ml relative to that of the positive control, ascorbic acid, being <200 µg/ml.
 Conclusion: The ESE from the rosehip seed press cake was found to be very promising as a food supplement since 50% of the 16 screened phyto-constituents were present in high concentrations; 6.25% were detected in moderate concentrations and another 6.25% were present in low concentrations. Furthermore, the ESE exhibited antioxidant properties. Further studies are recommended to obtain more information on its composition and suitability additive in animal feed or as a food supplement.

Analysis of phytochemical profile, ferric reducing power, H2O2 scavenging activity and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of extracts from aerial parts of <em>Pseudognaphalium undulatum</em>

“…Preliminary phytochemical screening of the DCM and methanolic extracts of P. undulatum for the presence of saponins, tannins, phlobatannins, terpenoids, phenolics, flavonoids, quinones, sterols and reducing sugars was carried out using standard methods reported in literature [11,12] .…”

“…The total phenolic content, TPC, of the dichloromethane and methanolic extract of P. undulatum was evaluated using a method described in literature [11] . Briefly, 10.0mg of gallic acid was dissolved in 10.0 ml of absolute methanol to form a 1000µg/ml stock solution.…”

“…The total flavonoid content, TFC, of the dichloromethane and methanolic extract of P. undulatum was determined using a method reported in literature [11] . The method involved the use of aluminum chloride calorimetric method whereby, various dilutions; 25, 20, 15, 10 and 5 µg/ml, of quercetin standard were obtained from a 25 µg/ml stock solution prepared by dissolving 25.0mg with 1.0 ml of absolute methanol.…”

“…5-hydroxy-3,7,8-tetramethoxyflavone and eight terpenoids (diterepens) viz. 8-epi-sclareol, 13-epicyclosclareol, ent-Kauran-16-ene, ent-Kaura-16-en-19-al, ent-Kaur-16-19-oic acid, 15 -hydroxy-ent-Kaur-16-ene-19-oic acid, ent-Kaur-9 (11),16-dien-19-oic acid and 15β-hydroxywedeliasecco Kaurenolide were identified [8] . Flavonoids and terpenoids were qualitatively detected in our study.…”