Phytochemical Screening and Evaluation of Antibacterial Activity of Ruta graveolens L. - A Medicinal Plant Grown around Mekelle, Tigray, Ethiopia

Amabye, Teklit Gebregiorgis; Shalkh, Tanveer Mahamadail

doi:10.4172/2329-6836.1000195

Cited by 21 publications

(18 citation statements)

References 22 publications

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“…The phytochemical analysis of essential oil of aerial parts of R. graveolens grown in different parts of the world has shown varied chemotype and the majority of the essential oil has 2-undecanone, 2-nonanone and 2-nonanol was the predominant compound. Similar results were found in R. graveolens found in Tunisia [21], Egypt [22,23], Ethopia [24,25] and Brazil [26]. In Iran, the plant essential oil showed that 2-undecanone (33.9%), 2-heptanol acetate (17.5%) and 1-dodecanol (11.0%) were major compounds [27].…”

Section: Discussionsupporting

confidence: 72%

Gc-MS Analysis and in Vitro Antioxidant Activities of Ruta Graveolens L. From Western Ghats Region –South India

Kathirvel¹,

Poonkodi²,

Gomathi³

et al. 2017

Asian J Pharm Clin Res

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Objective: The present study examines the chemical composition of essential oil of Ruta graveolens and antioxidant activity of petroleum ether and methanol extracts of dry leaves of R. graveolens which belongs to Rutaceae family found in many parts of the India. There are few reports are available for its essential oil in India. Methods:The fresh leaves were hydrodistilled by Clevenger type apparatus, the obtained essential oil was analyzed using gas chromatography-mass spectrometry (GC-MS). The petroleum ether and methanol extracts were tested for antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) methods. Results:The GC-MS results showed that 2-nonanol (34.25), 2-nonanone (32.15) and nonyl ester (15.43) are the major components and some of the other minor compounds are 2-dodecanone (2.77), tridecanone (2.54) and tumeronol (2.16). The preliminary phytochemical investigation of petroleum ether and methanol extracts showed the presence of carbohydrates, phenolic compounds, flavonoids, furanocoumarins, fatty acids and alkaloids. Hence, an attempt was made to find the possible sources for future novel antioxidants in food and pharmaceutical formulations. Conclusions:GC-MS studies indicated that 2-nonanone (32.15) and nonyl ester (15.43) are the major components. The methanol extract showed highest inhibitory concentration values for DPPH (19.50±1.25 µg/ml) and ABTS (48.32±0.9 µg/ml) and petroleum ether extract showed moderate activity, both results are comparable with standard employed for each method.

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Section: Discussionsupporting

confidence: 72%

Gc-MS Analysis and in Vitro Antioxidant Activities of Ruta Graveolens L. From Western Ghats Region –South India

Kathirvel¹,

Poonkodi²,

Gomathi³

et al. 2017

Asian J Pharm Clin Res

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“…It is cultivated as a medicinal and ornamental herb in many countries including Iran and India (Kannan and Babu 2012). The species has been used in America, Asia, and Europe for the treatment of many diseases having various pharmacological activities (Diwan et al 2012, Gentile et al 2015, Amabye and Shalkh 2015, Malik et al 2017. More than 100-120 compounds have been identified up to now from R. graveolens root and aerial part.…”

Section: N U S a N T A R A B I O S C I E N C E 11mentioning

confidence: 99%

Effects of Ruta graveolens total and flavonoids extracts on rat blood glucose, cholesterol, triglycerides and urea comparing synthetic drugs

et al. 2019

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Noori M, Jafari M, Azimi H, Node-Farahani M. 2019. Effects of Ruta graveolens total and flavonoids extracts on rat blood glucose, cholesterol, triglycerides and urea comparing synthetic drugs. Nusantara Bioscience 11: 23-29. Rue (Ruta graveolens L.: Rutaceae) is a medicinal plant that is used to treat many diseases in America, Asia and Europe due to its pharmaceutical properties. So far more than 120 different phytochemicals such as flavonoids have been extracted and identified from the species, which make it valuable in traditional medicine and manufacturing of synthetic drugs. Due to its endemic and high potential pharmacological effects, this study examined the oral effect of each of its total, flavonoids and non-flavonoids extracts on reducing blood glucose, cholesterol, triglycerides and urea in STZ-diabetic rats comparing to control and Atorvastatin, Allopurinol, Metformin as synthetic drugs. Aerial part of the plant was collected from around Yazd, Iran and dried in shade, crushed and extracted in 70% ethanol in three steps. Total and flavonoid extracts were prepared after rotary evaporation, then were used for making required doses after calculation. The plant flavonoids were identified using two-dimensional, thin-layer chromatography and TLC Scanner3 methods. Atorvastatin, Allopurinol and Metformin were obtained and also appropriate doses were prepared. Thirty-six adult male Wistar rats were divided in 12 groups (n=3): control, synthetic drugs control, total and flavonoids control, STZ-diabetic control, diabetic treated with synthetic drugs, total and flavonoids extracts, then weighed. All rats were treated orally at a specific time for 2 weeks. Subsequently, the secondary rat weights were determined and blood donation was performed after anesthesia. Then, blood serum glucose, cholesterol, triglycerides and urea were determined by spectrophotometry using commercial enzymatic chlorometric assay kits. All data were analyzed using EXCEL and SPSS software. Results showed aerial part of R. graveolens contains flavones C and C-/O glycosides, flavonoid sulphates and had no aglycones. Apigenin, chrycin, isorhamnetin, kaempferol, myricetin, quercetin, rutin and vitexin were found in the species aerial part. This plant had 329/99 gr/kgDW of effective material and 257.7 g/kgDW of flavonoids. The lowest blood glucose levels were observed in treated Metformin and flavonoids control rats respectively. Diabetic rats treated with R. graveolens total extract had the lowest blood cholesterol, urea and glucose levels. The highest renal damage was observed in control diabetic rats and the least renal damage was observed in the STZ-diabetic rats treated with the plant total extract. These results indicate that R. graveolens extract and its rutin contained antihyperglycemia, antihyperlipidemia, insulinity and protective properties.

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“…Various infections and disorders are caused by bacterial and fungal pathogens such as Aspergillus, Bacillus, Candida, Cryptococcus, Klebsiella, Proteous, Pseudomonas, Salmonella, Staphylococcus and Trichophyton (Bibi et al, 2011). The natural remedies and medicinal plants are the main resources for the physicians, especially in developing countries, depend on plants for medicines (Amabye and Shalkh, 2015). The importance of plants to homeopathy and modern medicine is correlated to their chemical constituents such as such as terpenoids, phenolics, alkaloids, flavonoids, amino acids, saponins, glycosides, diterpenes, triterpenes and their compatibility with the human body.…”

Section: Introductionmentioning

confidence: 99%

Extraction and Phytochemicals Determination of Traditional Medicinal Plants for Anti-microbial Susceptibility Test

Firew¹,

Petros²,

Abdurezak³

2020

Health Sci

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The study were conducted at Dilla University, College of Natural Sciences, Biological Sciences laboratories. Background: Ethiopia is a country with many ethnic groups, cultures and beliefs which in turn have contributed to the high diversity of traditional health care knowledge and practices of traditional medicine from local growth plants, animals and minerals for various physical and mental disorders of human and livestock population that passed from generation to generation for centuries. Medicinal plants contributors to pharmaceutical, agricultural and food industries in the world. The use of medicinal plants in the industrialized societies has been traced to extraction and development of several drugs used in order to heel some diseases having inhibiting effect against pathogenic microorganism. Objective: The main objective of this study was Extraction and Phytochemicals determination of traditional medicinal plants for anti microbial susceptibility test. Methodology: The extraction and identification of some phytochemicals crude compound which used for antimicrobial susceptibility test from plant sample such as Ocimum lamiifolium (OL), Croton maerosth (Cm) and Ruta chalepesis (RC) were conducted. Plant samples are collected, powdered using mortal and pistil and extracted using ethanol and some susceptibility tests were performed to identify some phytochemicals compound. Result: The main result of Antimicrobial activity test showed that the crude extract of OL has the highest zone of inhibition. The highest yield of crude extract (38.21%) was obtained from Croton maerosth (CM) which followed by Ruta chalepesis (RC) (32.43%). However, the lowest yield (28.37%) was obtained from Oscpmum lamifolium (OL). Conclusion: Traditional Medicine is used by many people to managing numerous conditions; it’s accessible and effective on antimicrobial activity. Therefore, it plays a significant role by reducing life-threatening ailments of people and other animals.

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Phytochemical Screening and Evaluation of Antibacterial Activity of Ruta graveolens L. - A Medicinal Plant Grown around Mekelle, Tigray, Ethiopia

Cited by 21 publications

References 22 publications

Gc-MS Analysis and in Vitro Antioxidant Activities of Ruta Graveolens L. From Western Ghats Region –South India

Gc-MS Analysis and in Vitro Antioxidant Activities of Ruta Graveolens L. From Western Ghats Region –South India

Effects of Ruta graveolens total and flavonoids extracts on rat blood glucose, cholesterol, triglycerides and urea comparing synthetic drugs

Extraction and Phytochemicals Determination of Traditional Medicinal Plants for Anti-microbial Susceptibility Test

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