Phytochemical Screening and Hepatoprotective Effect of &lt;i&gt;Alhagi maurorum &lt;/i&gt;Boiss (Leguminosae) Against Paracetamol-Induced Hepatotoxicity in Rabbits

Rehman, Jalil Ur; Akhtar, Naveed; Khan, Muhammad Younus; Ahmad, Khalil; Ahmad, Mukhtiar; Sultana, Sabira; Asif, Hafiz Muhammad

doi:10.4314/tjpr.v14i6.13

Cited by 23 publications

(19 citation statements)

References 12 publications

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“…5-8). Similar histopathological observations with high dose ParCM have also been reported (Naveed and Ibrar, 2014;Rehman et al, 2015;Rehman et al, 2013). The PoHF used in the study for evaluation of hepatoprotective effects caused a significant decline in serum levels of liver enzymes and total bilirubin, compared to levels for group B, indicating that the herbal product positively prevented the deteriorating effects of ParCM on the liver (Table. 1, Figs.…”

Section: Discussionsupporting

confidence: 71%

“…The measure of ALT, AST, ALP and total bilirubin in ParCM intoxicated group B was significantly (P<0.05) higher than in the untreated group A (Table 1, Figs. 1-4), demonstrating the damaging effects of ParCM on hepatic cell membranes from enzymes residing within the liver cells and leaking out of cell boundaries in the bloodstream, as reported by Raja et al (2007) (Raja et al, 2007) and Jain & Singha (2012) (Jain and Singhai, 2012 (2014), Rehman et al (2015), and Mumtaz et al (2015) (Ahmad et al, 2010;Mumtaz et al, 2015;Naveed and Ibrar, 2014;Rehman et al, 2015;Rehman et al, 2013). The hepatotoxic effects of ParCM were further verified by histopathological analyses.…”

Section: Discussionmentioning

confidence: 74%

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Hepatoprotective effect of a polyherbal formulation and ascorbic acid in paracetamol induced hepatic damage in rabbits

Fiaz

Shakir

et al. 2017

Biomed. Res. Ther.

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Background: The hepatoprotective effect of a polyherbal formulation was evaluated in female rabbits. The herbal formulation was used alone and in combination with ascorbic acid (AsAc) in animals with paracetamol (ParCM)-induced hepatic damage. Methods: The study design included five groups, each comprised of five animals. Group A was the control group (untreated; given only routine diet) while group B was given a single oral dose of ParCM (2 g/kg) on day 9. Groups C, D and E were pretreated with polyherbal formulation (PoHF; 500 mg/kg), ascorbic acid (AsAc; 200 mg/kg) and PoHF (500 mg/kg) combined with ascorbic acid (AsAc; 200 mg/kg), respectively for 9 consecutive days. On the last day (day 9), after 30 minutes of routine treatments, a single dose of ParCM (2 g/ kg) was administered in groups C, D and E. Animals were sacrificed 24 hours after the last treatment. Blood and liver samples were collected from all animals. Serum was separated from the blood samples and subjected to biochemical tests for liver biomarker analysis. The biomarkers included alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin. Elevation of enzyme markers was considered an indicator of hepatocellular injury. Results: Serum levels of liver enzymes and total bilirubin were elevated significantly in group B when compared to group A. The level of serobiochemicals significantly dropped in group C but increased significantly in group D, as compared to group B.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 74%

Hepatoprotective effect of a polyherbal formulation and ascorbic acid in paracetamol induced hepatic damage in rabbits

Fiaz

Shakir

et al. 2017

Biomed. Res. Ther.

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“…The results indicate that the extract assisted to maintain the membrane integrity of hepatocytes against paracetamol intoxication (Rehman et al, 2015). It can be proposed that the pretreatment with the extract reduced paracetamol-induced lipid peroxidation due to the presence of natural anti-oxidants such as quercetin, gallic acid and kaempferol (Weng et al, 2011).…”

Section: Discussionmentioning

confidence: 89%

Hepatoprotective potential and chemical characterization of Artocarpus lakoocha fruit extract

Saleem

Asif

Akhtar

et al. 2018

Bangladesh J Pharmacol

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Artocarpus lakoocha fruits are widely consumed as food. The study was aimed at evaluating its hepatoprotective activity and chemical constituents. The extract was analysed by HPLC for the presence of flavonoids and phenolic compounds. Hepatoprotective potential was determined in mice following 8 days of extract or silymarin (standard therapy) administration. Hepatotoxicity was induced by administration of paracetamol (500 mg/kg). The blood and liver of treated and untreated mice were collected 24 hours post-paracetamol intoxication. HPLC analysis confirmed the presence of chromatotropic acid, quercetin, gallic acid, vanillic acid, cinnamic acid, ferulic acid and kaempferol. Acute toxicity study showed no observed effect at more than 2,000 mg/kg. The fruit extract prevented the rise in liver function tests and paracetamol related histopathological alterations. The hepatoprotective activity of extract was dose-dependent. This study confirms the preventive effect of methanolic extract of monkey jack fruits against paracetamol-induced liver toxicity.Video Clip of Methodology:7 min 25 sec: <a href="https://www.youtube.com/v/oyuEIJc8pqU">Full Screen</a> <a href="https://www.youtube.com/watch?v=oyuEIJc8pqU">Alternate</a>

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“…Homogenates were placed at 4 °C for 20 min, and finally centrifuged at 3500 rpm for 15 min at 4 °C and supernatants were divided into aliquots and stored at −20 °C until assessed for different enzymes. According to the reported method [ 43 ] activities of glutathione peroxidase (GPx) and total content of reduced glutathione (GSH) level in the supernatant obtained from liver and kidney tissues were analyzed. Thiobarbituric acid reactive substance (TBARS) assay was used for estimation of and malondialdehyde (MDA) level (lipid peroxidation) [ 6 ].…”

Section: Methodsmentioning

confidence: 99%

“…The stained slides were cleaned properly and observed under microscope to see alteration in pancreatic tissues (architecture of STZ-induced diabetic group) and treated (glibenclamide/extract) groups. The samples were processed according to reported protocols [ 43 ].…”

Section: Methodsmentioning

confidence: 99%

Curative Effect of Catechin Isolated from Elaeagnus Umbellata Thunb. Berries for Diabetes and Related Complications in Streptozotocin-Induced Diabetic Rats Model

et al. 2020

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In this study, catechin (CTN) isolated from Elaeagnus umbellata was evaluated for in vitro antioxidant potential and inhibition of carbohydrate digestive enzymes (α-amylase and α-glucosidase). The compound was also tested for its in vivo antidiabetic potential using Sprague-Dawley rats as experimental animals. The effects of various doses of catechin in STZ (Streptozotocin) induced diabetic rats on fasting blood glucose level, body weight, lipid parameters, hepatic enzymes, and renal functions were evaluated using the reported protocols. The CTN exhibited the highest percent antioxidant for free radical scavenging activity against DPPH and ABTS free radicals, and inhibited the activity of carbohydrate digestive enzymes (with percent inhibition values: 79 ± 1.5% α-amylase and 80 ± 1.1% α-glucosidase). Administration CTN and standard glibenclamide significantly decreased the fasting blood glucose level and increased the body weight in STZ-induced diabetic rats. CTN significantly decreased the different lipid parameters, hepatic, and renal function enzyme levels along with Hb1c level in diabetic rats, while significantly increasing the high-density lipoprotein (HDL) level with values comparable to the standard glibenclamide. Further, the altered levels of glutathione and lipid peroxides of liver and kidney tissues were restored (by CTN) to levels similar to the control group. CTN significantly increased the antioxidant enzyme activities, total content of reduced glutathione, and reduced the malondialdehyde (MDA) level in rat liver and kidney tissues homogenates, and also corrected the histopathological abnormalities, suggesting its antioxidant potential.

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Phytochemical Screening and Hepatoprotective Effect of <i>Alhagi maurorum </i>Boiss (Leguminosae) Against Paracetamol-Induced Hepatotoxicity in Rabbits

Abstract: Purpose: To evaluate the hepatoprotective activity of aqueous-ethanol (30:70 %)

Cited by 23 publications

References 12 publications

Hepatoprotective effect of a polyherbal formulation and ascorbic acid in paracetamol induced hepatic damage in rabbits

Hepatoprotective effect of a polyherbal formulation and ascorbic acid in paracetamol induced hepatic damage in rabbits

Hepatoprotective potential and chemical characterization of Artocarpus lakoocha fruit extract

Curative Effect of Catechin Isolated from Elaeagnus Umbellata Thunb. Berries for Diabetes and Related Complications in Streptozotocin-Induced Diabetic Rats Model

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