Phytochemical screening and HPTLC fingerprinting of different parts of Solanum indicum L.: A dashmool species

Saxena, Hari Om; Parihar, S. S.; Pawar, Ganesh; Choubey, Santosh Kumar; Dhar, Pranav

doi:10.22271/phyto.2021.v10.i1aa.13633

Cited by 7 publications

(10 citation statements)

References 18 publications

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“…HPTLC analysis was carried out to develop the fingerprint profile of crude FEE as explained by Syed et al . [56] with a bit of modification. Fingerprinting was achieved through the use of the mobile phase, viz.…”

Section: Methodsmentioning

confidence: 99%

Exploration of the inhibitory effect of Cassia fistula on quorum sensing mediated virulence factor production and biofilm activity in Pseudomonas aeruginosa: an in vivo study in model organism Caenorhabditis elegans

Peerzada

Shah

Kharkar

et al. 2023

Journal of Medical Microbiology

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Introduction. Resistance to antibiotics is leading to challenges in the treatment of microbial diseases. One amongst the various approaches to control these pathogens is quorum sensing (QS), which is used to rectify resistance issues. Blocking the bacterial QS circuit is the most reliable anti-virulence therapy to control pathogenicity-associated genes. Pseudomonas aeruginosa is a contagious bacterium that proliferates in the host by using signalling molecules like acyl-homoserine lactones; these molecules generate and disseminate toxins and virulence factors for increasing host infection. Hypothesis. The herb Cassia fistula is known to have antimicrobial, antidiabetic, anti-inflammatory, antitumor medicinal properties amongst others. We hypothesize that its crude extracts will inhibit the QS circuit of Pseudomonas aeruginosa (P. aeruginosa). Aim. The research work was aimed at evaluating anti-quorum sensing and anti-biofilm activity of various crude extracts from Cassia fistula against P. aeruginosa . Methodology. Various extraction methods and solvents were availed for maximum separation, and the extracts were screened for anti-quorum sensing activity. The most potent Fruit Ethyl acetate (FEE) extract at non-inhibitory concentrations was found to interrupt both short-chain (RhlI/R) and long-chain (LasI/R) QS circuits and other virulence factors (P<0.05) such as elastase, protease, rhamnolipids and pyocyanin levels in P. aeruginosa . Biofilm inhibitory properties of FEE were demonstrated using atomic force microscopy, scanning electron microscope and confocal laser microscope. Caenorhabditis elegans infection model (Paralytic assay) was developed to determine the protective role of FEE by reducing the pathogenicity of P. aeruginosa . Results. The study results suggest that hot crude FEE extract interfered in the QS circuit, leading to comprehensive debilitation of QS-controlled virulence factors. The extract reduced virulence factor production in P. aeruginosa at 4 mg ml-1 concentration whilst paradoxically promoting biofilm formation. Possibly, higher sugar content in the extract promoted clump formation of biofilm architecture by increasing exopolysaccharide production. Moreover, in vivo analysis of bacterial pathogenesis on Caenorhabditis elegans reveals a drastic increase in survival rates in FEE treated worms compared to untreated control. Conclusions. FEE showed promising QS inhibitory activity against P. aeruginosa . In the future, additional purification of crude FEE is required to remove carbohydrates, and pure isolated phytochemicals from FEE could be used as therapeutic agents to control QS-mediated infections in P. aeruginosa .

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Section: Methodsmentioning

confidence: 99%

Exploration of the inhibitory effect of Cassia fistula on quorum sensing mediated virulence factor production and biofilm activity in Pseudomonas aeruginosa: an in vivo study in model organism Caenorhabditis elegans

Peerzada

Shah

Kharkar

et al. 2023

Journal of Medical Microbiology

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“…Plants are a major source of several structurally important phytochemicals which may be classified into alkaloids, carotenoids, organosulphurs, phenols and phytosterols. 107 Amongst phenolic compounds, flavonoids are the largest and most studied compounds and may be found in fruits, vegetables, barks, roots, stems, flowers and grains ( Fig. 3D ).…”

Section: Small Bioactive Molecules For Te and Their Use In Scaffoldsmentioning

confidence: 99%

Mimicking growth factors: role of small molecule scaffold additives in promoting tissue regeneration and repair

Goonoo

Bhaw‐Luximon

2019

RSC Adv.

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The primary aim of tissue engineering scaffolds is to mimic the in vivo environment and promote tissue growth. In this quest, a number of strategies have been developed such as enhancing cell-material interactions through modulation of scaffold physico-chemical parameters. However, more is required for scaffolds to relate to the cell natural environment. Growth factors (GFs) secreted by cells and extracellular matrix (ECM) are involved in both normal repair and abnormal remodeling. The direct use of GFs on their own or when incorporated within scaffolds represent a number of challenges such as release rate, stability and shelf-life. Small molecules have been proposed as promising alternatives to GFs as they are able to minimize or overcome many shortcomings of GFs, in particular immune response and instability. Despite the promise of small molecules in various TE applications, their direct use is limited by nonspecific adverse effects on non-target tissues and organs. Hence, they have been incorporated within scaffolds to localize their actions and control their release to target sites. However, scanty rationale is available which links the chemical structure of these molecules with their mode of action. We herewith review various small molecules either when used on their own or when incorporated within polymeric carriers/scaffolds for bone, cartilage, neural, adipose and skin tissue regeneration.

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“…They can be easily isolated from herbs and dietary plants. A wide range of natural products and pharmaceuticals contain phenols in monomeric or polymeric forms [8] . Phenols also behave as versatile precursors for a diverse range of drugs, antioxidants, herbicides, agrochemicals and polymers [9] .…”

Section: Introductionmentioning

confidence: 99%

A Doubly Interpenetrated Cu(II)‐based Metal‐Organic Framework as a Heterogeneous Catalyst for the ipso‐Hydroxylation of Arylboronic Acids

et al. 2023

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We dedicate this paper to Professor Rajeev Gupta on the occasion of his 51 st birthday.A doubly interpenetrated Cu(II)-organic framework with formula and 4,4'-bpdc = [1,1'-biphenyl]-4,4'-dicarboxylate ion) has been synthesized and characterized with the help of several spectroscopic and analytical techniques including single crystal X-ray analysis. A single crystal X-ray analysis reveals that 1 exhibit interpene-trated two-dimensional sheet-like structure containing elongated channels of cross-section 11.09 × 31.22 Å 2 along the aaxis. Finally, 1 has been exploited as a heterogeneous catalyst for the ipso-hydroxylation of arylboronic acids yielding up to 99 % of the respective phenolic product. Importantly, the catalyst can be reused for five successive cycles without having a significant loss in its catalytic efficiency.

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Phytochemical screening and HPTLC fingerprinting of different parts of Solanum indicum L.: A dashmool species

Cited by 7 publications

References 18 publications

Exploration of the inhibitory effect of Cassia fistula on quorum sensing mediated virulence factor production and biofilm activity in Pseudomonas aeruginosa: an in vivo study in model organism Caenorhabditis elegans

Exploration of the inhibitory effect of Cassia fistula on quorum sensing mediated virulence factor production and biofilm activity in Pseudomonas aeruginosa: an in vivo study in model organism Caenorhabditis elegans

Mimicking growth factors: role of small molecule scaffold additives in promoting tissue regeneration and repair

A Doubly Interpenetrated Cu(II)‐based Metal‐Organic Framework as a Heterogeneous Catalyst for the ipso‐Hydroxylation of Arylboronic Acids

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