Phytochemical Screening and HPTLC Fingerprinting of Anogeissus Acuminata Extracts

Navale, Archana; Patel, Komal S; Paranjape, Archana

doi:10.7897/2277-4343.07288

Cited by 6 publications

(3 citation statements)

References 7 publications

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“…The colour intensity, change in colour and appearance of solids in reaction mixture was observed 37 . The qualitative results are expressed as (+) for the presence and (-) for the absence of phytochemicals.…”

Section: Qualitative Estimationmentioning

confidence: 99%

Qualitative Estimation of Seeds of Momordica Charantia Linn. (Cucurbitaceae) and Characterization of Gallic Acid Isolated From Its Alcoholic Extract

Katiyar¹,

Singh²,

Ali³

2016

Int. J. Res. Ayurveda Pharm.

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The current investigation engrosses the qualitative estimation of the seeds of Momordica charantia Linn. (Cucurbitaceae) and characterization of gallic acid isolated from its alcoholic extract. The seeds were successively extracted in petroleum ether, chloroform, ethanol and water in a Soxhlet Apparatus. Each of the extract was subjected to the preliminary phytochemical screening using the standard qualitative identification tests. The extract showing the presence of maximum phytoconstituents was utilized for the isolation of compounds by column chromatography and characterization was performed by using various spectral data analysis i.e., IR, 1H NMR, 13C NMR and DART-MS. The alcoholic extract exhibited the presence of maximum number of phytoconstituents. Elution of the column with 0.2% Methanol in Chloroform gave a pale yellow, crystalline compound -Gallic Acid (3, 4, 5-trihydroxy benzoic acid). The diverse nature of chemical constituents present in the seeds of Momordica charantia Linn. (Cucurbitaceae) and the isolated compound-Gallic Acid can be further explored for their pharmacological potential which might be of immense value to mankind. This compound might act as the chromatographic marker and shall be beneficial in identifying and assuring the quality of the crude drug.

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Section: Qualitative Estimationmentioning

confidence: 99%

Qualitative Estimation of Seeds of Momordica Charantia Linn. (Cucurbitaceae) and Characterization of Gallic Acid Isolated From Its Alcoholic Extract

Katiyar¹,

Singh²,

Ali³

2016

Int. J. Res. Ayurveda Pharm.

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“…It has shown potent anti-oxidant effects in several studies (4,5). It has rich flavonoid and tannin content (6), some of which compounds have demonstrated beneficial pharmacological actions. Thus, if used for treatment of diabetes mellitus, it may have renal protective effect and may prevent development of diabetic nephropathy in long-term.…”

Section: Introductionmentioning

confidence: 99%

Beneficial effects of Terminalia phillyreifolia (Van Heurck & Müll.Arg.) Gere & Boatwr. bark extract in streptozotocin induced hyperglycaemia and diabetic nephropathy in rats

Navale

Patel

Paranjape³

2021

J Nephropathol

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Introduction: Renal damage is a common clinical manifestation in diabetic patients. Therefore, nephroprotective effect is a desirable property for an antidiabetic agent. Natural compounds belonging to Terminalia phillyreifolia (TP) owing to their potent antioxidant and anti-inflammatory activity may prove to be such agents. Objectives: The objectives of the present study were to evaluate methanolic extract of TP bark for its antihyperglycemic, antioxidant and nephroprotective effect in streptozotocin (STZ) induced diabetes mellitus and nephropathy in rats. Materials and Methods: Diabetic male Wistar rats were divided into five groups; namely, normal control, disease control, standard (NPH insulin, subcutaneously), BE100 (bark extract 100 mg/kg, p.o.) and BE300 (bark extract 300 mg/kg, orally). Treatment was continued for 8 weeks. Plasma glucose levels, oxidative stress parameters, serum creatinine levels, blood urea nitrogen (BUN) levels, per day urine output, urinary protein excretion (UPE) and kidney hypertrophy index were determined at appropriate time points. Results: Untreated animals developed severe hyperglycemia and major disturbance in renal function. Rats in standard and BE treated groups had significantly lower plasma glucose levels and oxidative stress markers as compared to disease control animals. BE rats also exhibited nearly normal urine volumes indicating better glomerular filtration rate. They had lower urinary protein, serum creatinine, BUN levels and lower renal hypertrophy index as compared to untreated animals. Conclusion: TP Bark extract corrected the hyperglycaemia and exerted protective effect against diabetes induced renal damage in rats, which may be partly due to its anti-oxidant effect. Therefore, TP extract can be further evaluated as potential antidiabetic therapy

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“…Aerial parts of the tree are also used for treatment of diabetes mellitus 8 . A. acuminata leaves are used in traditional and tribal medicine of Andhra Pradesh to treat painful inflammatory conditions 9 . Hence the present study was aimed to evaluate its scientific validity 10 .…”

Section: Introductionmentioning

confidence: 99%

Phytochemical and Pharmacognostical Studies of Anogeissus Acuminata

Yadav¹,

Irchhiaya²,

Ghosh³

2019

J. Drug Delivery Ther.

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Medicinal plants are one of the oldest valuable sources of medicine provide by nature to mankind. Medicinal plants are one of the important sources of research of new drugs. Many medicinal plants and herbs hold their prestigious position in the field of natural medicine among which Anogeissus acuminata belonging to family Combretaceae was vital one. In the present study was intended to evaluate various physicochemical, phytochemical standards like TLC, HPTLC and Pharmacognostic parameters which will be helpful to ensure the purity, safety and efficacy of the medicinal plant. Anogeissus acuminata was subjected to preliminary phytochemical screening, done to estimate as foreign organic matter, morphological evaluation. Total ash values, physicochemical, Thin layer chromatography (TLC), HPTLC phytochemical analysis and secondary metabolites present in the plant were investigated for the extract. All physiochemical properties were found within the measureable amount as foreign organic matter less than 1% w/w, Total ash values was 12 % w/w with respect to air-dried crude drug. Moisture content was 3.074. Thin layer chromatography (TLC), HPTLC studies were carried for the better isolation and identification of the different components of ethanolic extract. HPTLC carried out in the solvent system - Toluene: Ethyl acetate: Acetic acid (4:3:1). The report of HPTLC indicates the presence of sixteen spots. Preliminary phytochemical analysis confirmed the presence of plant extracts revealed the presence of alkaloids, steroids, terpenoids glycosides, tannins and phenolic compounds. KEYWORDS: TLC, HPTLC, Phytochemical characterization, Anogeissus acuminata

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Phytochemical Screening and HPTLC Fingerprinting of Anogeissus Acuminata Extracts

Cited by 6 publications

References 7 publications

Qualitative Estimation of Seeds of Momordica Charantia Linn. (Cucurbitaceae) and Characterization of Gallic Acid Isolated From Its Alcoholic Extract

Qualitative Estimation of Seeds of Momordica Charantia Linn. (Cucurbitaceae) and Characterization of Gallic Acid Isolated From Its Alcoholic Extract

Beneficial effects of Terminalia phillyreifolia (Van Heurck & Müll.Arg.) Gere & Boatwr. bark extract in streptozotocin induced hyperglycaemia and diabetic nephropathy in rats

Phytochemical and Pharmacognostical Studies of Anogeissus Acuminata

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