Phytochemical screening, antimicrobial activity, in vitro and in vivo antioxidant activity of Berberis lycium Royle root bark extract

Abstract

Antioxidants are materials that scavenge or remove free radicals from living systems. The oxidation process ends in the production of free radicals. These free radicals are the chief birthplace of cancerous cells. Antioxidizing agents remove free radical intermediates by terminating oxidation processes by being oxidized themselves. On the other hand, infectious diseases affect the world on a large scale. To fight these diseases several synthetic compounds have been used. Plant based medications play important role in this regard. So, the current research aimed to investigate the antibacterial and antioxidant effect of Berberis lycium Royle root bark (BLR) extract. Berberis lycium Royle was used for phytochemical analysis and also as antimicrobial and antioxidant agents. The antimicrobial activity was evaluated by the agar well diffusion method. Current study revealed that BLR was rich in phytochemicals and toxic against tested pathogenic bacteria. BLR showed the highest activity against S. pyogenes (13.3±0.8 mm). The lowest antibacterial activity was reported against E. coli (0±0 mm). In case of minimum inhibitory concentration, it was observed that BLR with 10 μg/mL concentration showed the highest activity while 2.5 μg/mL of BLR showed the least inhibitory activity. The highest In vitro antioxidant activity was recorded as 65% at 100 µg/mL. In case of in vivo antioxidant activity level of CAT, GSH and SOD were decreased while that of MDA was enhanced in groups treated with CCl4 as compared to the control group. BLR extract treatment reversed all these changes significantly. Current results indicate that BLR is effective against bacterial pathogens and also has antioxidant potential.

Effect of Mauritia flexuosa L. leaf extract on Staphylococcus aureus and Staphylococcus haemolyticus biofilms adhered to stainless steel surface

“…The antibacterial effect of Berberis lycium Royle root bark aqueous extract was evaluated and showed zones of inhibition at 10±1.5 mm to S. aureu and 13.3±0.8 mm to Streptococcus pyogenes. the extrac was effective against this bacterial pathogens and also had antioxidant potential (Mughal et al, 2024).…”

Antibacterial and Wound Healing Efficacy of Silver Nanoparticles Synthesized from Root Bark ofBerberis lyciumRoyle

“…Total trials were conducted in compliance with international laws and adhered to the ethical guidelines set forth by the University for the humane treatment of experimental animals, as outlined in our prior research. [18][19][20][21][22][23][25][26][27] The Ethics Committee of the Faculty of Science at GC University, Lahore, Pakistan, granted ethical approval for this study under approval number GCU-IIB 118.…”

“…The procedure described in our previous studies [18,20,21] was used to prepare the aqueous extract from shade-dried root bark of B. lyceum Royle. To be exact, 10 g of the powder were mixed with 100 mL of distilled water, and the combination was then incubated for 30 min at 50 °Cin a water bath.…”

“…Traditional medicine needs to be rediscovered based on our current understanding of allopathic or modern medicine. [ [17][18][19] In current research, instead of using silver alone, phytoextracts from B. lyceum Royle coupled with AgNPs were used for the bactericidal and wound healing activity. AgNPs coupled with phytoextracts have gained more interest because of their action on bacterial pathogens and promotion of wound healing.…”

“…[16] Berberis lyceum Royle has been used to treat different diseases such as jaundice, diarrhea, internal wounds and diabetes. [17,18] Diverse portions of B. lyceum Royle have been used in numerous customary formations to treat different diseases. The traditional medicine systems have created many useful clues for the development of modern medicine to treat many ailments.…”