Phytochemical screening, antimicrobial and antioxidant activity determination of Trigonella foenum-graecum seeds

Khan, Naeem Hasan; Hwa, Chua Yoke; Perveen, Nabila; Paliwal, Neeraj

doi:10.15406/ppij.2019.07.00249

Cited by 10 publications

(17 citation statements)

References 13 publications

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“…The results of the present study showed that N. sativa seeds are great reservoirs of medicinally active ingredients (phytochemicals) and they are consistent with earlier phytochemical screening studies done on different solvent extracts and oil of N. sativa seeds which also reported that N. sativa seeds or extracts and oil are rich in the above-mentioned phytoconstituents [1,31]. Bioactive constituents such as alkaloids, flavonoids, phenols, tannins and terpenoids are known to elicit broad antimicrobial responses against bacteria, fungi, viruses and parasites [3,17,32,33]. A number of studies documented that these phytochemicals also…”

Section: Qualitative Screening Of Phytochemicals From Different Extra...supporting

confidence: 81%

“…Irregular zones of inhibition were observed in this study around the discs ( Fig 6 ) and Khan et al [ 17 ] had reported that it may be due to improper diffusion of the extracts or uneven growth of the bacteria at a particular area of the nutrient agar. Most of these sources of variations (i.e., the type of extractant used, extraction technique employed, the type of pathogenic bacteria strains used and variation due to error) were confirmed by the two-factor ANOVA statistical analysis done on mean values of replication of the zones of inhibition of different extracts and/or oil of N .…”

Section: Resultssupporting

confidence: 53%

“…sativa seeds or extracts and oil are rich in the above-mentioned phytoconstituents [ 1 , 31 ]. Bioactive constituents such as alkaloids, flavonoids, phenols, tannins and terpenoids are known to elicit broad antimicrobial responses against bacteria, fungi, viruses and parasites [ 3 , 17 , 32 , 33 ]. A number of studies documented that these phytochemicals also possess many pharmacological properties, including but not limited to anticancer, antioxidant, anti-inflammatory, cytotoxicity, anti-diarrheal, anti-hemostatic, anti-hemorrhoidal, anti-apoptosis, anti-aging, and growth regulation [ 31 , 34 ].…”

Section: Resultsmentioning

confidence: 99%

“…The obtained extracts were subjected to phytochemical screening using standard methods [ 3 , 17 , 18 ] that are summarized below. The general reactions in this assay signaled the presence or absence of major phytochemical phytoconstituents in the extracts of N .…”

Section: Methodsmentioning

confidence: 99%

“…The extracts or preparations of N. sativa seeds, oil extracted with hexane, oil extracted with absolute ethanol, methanol extract and aqueous extract (undiluted and diluted 1:1 in ethylene glycol (LD Didactic) were tested for their inhibitory activity against the pathogenic bacteria by pippeting 10 μL of each preparation or extract onto the 5 mm paper discs. The volume of each preparation delivered onto each disc was selected on the basis that plants may exhibit antimicrobial properties when assayed at an adequate amount [17,25]. Distilled Water was used as a negative control in addition to the extracts while 5 mm discs of a known antibiotic (Streptomycin) was selected among others and used as a positive control for comparison.…”

Section: Antimicrobial Activity Of the Extracts Of N Sativa Seedsmentioning

confidence: 99%

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Phytochemical characterization and antimicrobial activity of Nigella sativa seeds

2022

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Nigella sativa is one of the medicinal plant species that gained popularity for a wide range of medicinal applications due to its seeds which are rich in phytoconstituents. Continuous scientific investigations on N. sativa seeds are needed to better understand its many medicinal potentials. This will also form a composition-based foundation that support several old and/or new case beneficial histories of its seeds. In this study, the antimicrobial activity of N. sativa seeds was phytochemically characterized and evaluated. Different extracts of N. sativa seeds were obtained by maceration and soxhlet extraction methods using different extraction solvents. The obtained extracts were tested using UV-Vis, FTIR, TLC, and GC-MS techniques. Antimicrobial analysis against pathogenic bacterial strains (E. coli, P. aeruginosa, S. aureus and B. subtilis) was carried out by disc diffusion method using different preparations of N. sativa seeds. The screening analysis revealed the presence of all the tested phytochemicals. FT-IR analysis of N. sativa seeds oil extracted with absolute ethanol revealed functional groups that are associated with active ingredients of medicinal value. The GC-MS chromatograms revealed different chemical constituents whose known bioactivities and/or applications are essential in the management of life-threatening infections. Different extracts of N. sativa seeds showed antimicrobial activity with different efficacy against the tested pathogenic bacterial strains. Therefore, this study shows that extracts of N. sativa seeds contain a variety of chemical components and functional groups linked to their antimicrobial properties, and they might be natural precursors of nutraceuticals.

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Section: Qualitative Screening Of Phytochemicals From Different Extra...supporting

confidence: 81%

Section: Resultssupporting

confidence: 53%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Antimicrobial Activity Of the Extracts Of N Sativa Seedsmentioning

confidence: 99%

See 3 more Smart Citations

Phytochemical characterization and antimicrobial activity of Nigella sativa seeds

2022

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A comprehensive perspective of traditional Arabic or Islamic medicinal plants as an adjuvant therapy against COVID-19

Ahmed

Jamil

Ismatullah

et al. 2023

Saudi Journal of Biological Sciences

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Restoration of Antibacterial Activity of Inactive Antibiotics via Combined Treatment with AgNPs

Ghaffar,

Javad,

Shah

et al. 2024

ACS Omega

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Antimicrobial resistance poses a huge threat to human health around the world and calls for novel treatments. Combined formulations of NPs and antibiotics have emerged as a viable nanoplatform for combating bacterial resistance. The present research work was performed to investigate the effect of combined formulations of AgNPs with streptomycin, cefaclor, ciprofloxacin, and trimethoprim against multidrug-resistant (MDR) isolates of Staphylococcus aureus and Klebsiella pneumoniae. AgNPs have been synthesized by using the Nigella sativa seed extract, and their characteristics were analyzed. AgNPs depicted concentration-dependent antibacterial effects, as the highest concentration of AgNPs showed the strongest antibacterial activity. Interestingly, AgNPs in conjugation with antibiotics showed an enhanced antibacterial potential against both S. aureus and K. pneumoniae, which suggested synergism between the AgNPs and antibiotics. Against S. aureus, streptomycin and trimethoprim in conjugation with AgNPs presented a synergistic effect, while cefaclor and ciprofloxacin in combination with AgNPs showed an additive effect. However, all of the tested antibiotics depicted a synergistic effect against K. pneumoniae. The lowest value of MIC (0.78 μg/mL) was shown by AgNPs-Stp against S. aureus, whereas AgNPs-Tmp showed the lowest value of MIC (1.56 μg/mL) against K. pneumoniae. The most important point of the present study is that both organisms (S. aureus and K. pneumoniae) showed resistance to antibiotics but turned out to be highly susceptible when the same antibiotic was used in combination with AgNPs. These findings highlight the potential of nanoconjugates (the AgNPs-antibiotic complex) to mitigate the present-day crisis of antibiotic resistance and to combat antimicrobial infections efficiently.

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Phytochemical screening, antimicrobial and antioxidant activity determination of Trigonella foenum-graecum seeds

Cited by 10 publications

References 13 publications

Phytochemical characterization and antimicrobial activity of Nigella sativa seeds

Phytochemical characterization and antimicrobial activity of Nigella sativa seeds

A comprehensive perspective of traditional Arabic or Islamic medicinal plants as an adjuvant therapy against COVID-19

Restoration of Antibacterial Activity of Inactive Antibiotics via Combined Treatment with AgNPs

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