Phytochemical Screening, Antioxidant, Antiulcer, Anti-Inflammatory and Analgesic Activity of the Aqueous Extract of Angelica archangelica

Abstract

The objective of this study is to evaluate the antioxidant, antiulcer, anti-inflammatory and analgesic activity of the aqueous extract of Angelica archangelica L., a medicinal plant of the traditional pharmacopoeia of Algeria. The aqueous extract showed a large amount of total polyphenols, tannins, chlorophyll a, b, carotenoids and a small amount of flavonoids with values of 80.16±14.3 mg EAG/g of extract, 3.12±2.01 mg EQ/g of extract, 226.10±4.50 mg EAT/g of extract, 6.10±50.62, 12.00±31.53 and 1.78±0.40 μg/mL respectively. In addition, the aqueous extract of A. archangelica showed a high ability to trap DPPH radical in with IC50 at 16.9±2.03μg/mL. However, the aqueous extract has a great protective effect against β-carotene degradation at 91.98±0.64% after 24 hours and significant ferrous ion chelation activity at46.2 ±1.53µg/mL. Furthermore, the anti-inflammatory activity was studied according to the protein denaturation inhibition method and, according to the results obtained, the extract of A. archangelica at concentrations of 250, 500 μg/mL and 1 mg/mL have ahigh antiinflammatory activity whose inhibition percentages are 78.85±5.31%, 86.65±2.70% and 89.89±0.58% respectively. The analgesic effect of the aqueous extract of A. archangelica, showed that the concentration 400 mg/mL has a greet abdominal cramps inhibitory effect in comparison with the concentration 200 mg/mL with a percentage of 98.28%. The antiulcer effect of the aqueous extract of A. archangelica was evaluated by the 70% ethanol-induced ulcer test. The results obtained reveal that the aqueous extract 200 and 400 mg/mL exerted a considerable effect of protecting the stomach at 86.55±3.51% and 82.82±2.18% respectively.

Free Radical Scavenging and Antinociceptive Activities of the Aqueous Extract from Matricaria chamomilla L. Flowers.

“…Such pain stimulus leads to the release of free arachidonic acid from the tissue phospholipid. The response is thought to be mediated by peritoneal mast cells, acid sensing ion channels and the prostaglandin pathway (Miraj et al, 2019;Mamache et al:, 2022). The results of this study have shown that M. chamomilla extract can induce analgesia in mice, by inhibiting the acetic mice acid-induced writhing.…”

“…Determination of antioxidant capacity of MCAqE was performed by a simple assay using the stable DPPH radical with the method described by Mamache et al (2022), where a volume of 50 μL of different dilutions of the plant extract was added to 1250 µL of DPPH solution (0.004%). After 30 min of incubation at room temperature, the absorbance was measured at 517 nm.…”

HPLC Profile and Different Pathways Involved in Achillea odorata-Induced Gastric Emptying and Intestinal Transit Delay

“…The aqueous extract was made following the methodology outlined by mamache et al 14 A 15 g of the powdered plant material was heated to a boil in 500 mL of water for ten (10) minutes, and then it was allowed to cool. The homogenate was then filtered using filter paper (Whatman size 2) and transferred into plates; it was dried at 38°C, 14 in an oven (MEMMERT UM 400 ref. P2209-1894, Germany).…”