Phytochemical, Toxicological and Pharmacological Studies of &lt;i&gt;Asiasari Radix&lt;/i&gt; et Rhizoma: A Review

Mahesh, R.; Kim, Sungjin

doi:10.4314/tjpr.v14i3.26

Cited by 17 publications

(12 citation statements)

References 50 publications

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“…Asiasari radix (AR) has long been used in traditional Korean and Chinese medicine to treat cough, toothache, headache, neuralgia, gingivitis, asthma, and allergies due to its anti-bacterial and analgesic effects [1]. Recently, the pharmacological roles of AR extracts (AREs) have been reported.…”

Section: Introductionmentioning

confidence: 99%

Ethanol extract of asiasari radix preferentially induces apoptosis in G361 human melanoma cells by differential regulation of p53

Park

Choi

Song

et al. 2019

BMC Complement Altern Med

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Background: In Korea and China, asiasari radix (AR) is widely used as a traditional anti-inflammatory and analgesic agent. After its skin-regenerating and hair loss-preventing activities were identified, several types of AR extracts were used for aesthetic purposes. Nevertheless, the effect of ARE on various types of skin cancers was not fully studied yet. Methods: In this study, we tested the effect of an ethanolic AR extract (ARE) on G361 human melanoma and HaCaT human keratinocyte cell lines. After ARE exposure, cell growth and the expression patterns of proteins and genes were monitored. Results: The ARE-mediated cell growth inhibition was greater in G361 cells than in HaCaT cells due to differences in its cell growth regulation effects. Interestingly, ARE treatment induced caspase-3-mediated apoptosis in G361 cells, but not in HaCaT cells. Furthermore, ARE reduced the expression of p53 and p21 proteins in G361 cells, whereas it induced their expression in HaCaT cells. ARE induced cell death in G361 cells through the reactive oxygen species (ROS)-dependent regulation of p53 and p21 in G361 cells. Microarray analysis showed that ARE regulates Mouse double minute 2 homolog (MDM2) and CASP8 and FADD-like apoptosis regulator (CFLAR) gene expression in G361 and HaCaT cells differently. Conclusion: The treatment of ARE preferentially induces apoptosis in melanoma cells by the ROS-dependent differential regulation of p53 level. Therefore, ARE can be used as a new medicinal option for melanoma.

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Section: Introductionmentioning

confidence: 99%

Ethanol extract of asiasari radix preferentially induces apoptosis in G361 human melanoma cells by differential regulation of p53

Park

Choi

Song

et al. 2019

BMC Complement Altern Med

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“…39,40 Previous studies have reported the chemical constituents of A. sieboldii, such as essential oils (eg, methyl eugenol, cineol, safrole, eucarvone, elemicin, and kakuol), lignans (eg, asarinin, xanthoxylol, and sesamin), and alkaloids (eg, aristolochic acid, aristolactam, and higenamine). [23][24][25] The contents of the compounds were compared between different collections of A. sieboldii using HPLC, ultra HPLC-quadrupole time-of-flight/mass spectrometry (MS), and gas chromatography (GC)-MS. 30,41,42 In addition, the contents of the essential oils of A. sieboldii were compared using GC according to the growth conditions and years of cultivation. 43,44 Other studies have proposed methods for the quantitative analysis of the components of A. sieboldii for quality control.…”

Section: Discussionmentioning

confidence: 99%

“…The major components of A. sieboldii are essential oil, lignans, and alkaloids, as reported in previous studies. 19,[23][24][25] The study of the components of traditional herbal medicines is very important to ensure the reliability of pharmacological and clinical research. 26 Because traditional herbal medicines have different chemical compositions, it is necessary to develop accurate and effective simultaneous quantification methods.…”

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confidence: 99%

Simultaneous Determination of 6 Antiallergic Components in Asarum sieboldii Using High-Performance Liquid Chromatography

Jang

Park

Kim

2020

Natural Product Communications

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Owing to the side effects of current drugs for treating atopic dermatitis (AD), a chronic disease in the skin, traditional herbal medicine is receiving much attention as an alternative treatment. Asarum sieboldii has traditionally been used to treat colds, fevers, headaches, coughs, neuralgia, chronic bronchitis, asthma, and allergies. In this study, 6 compounds (echinacoside, vanillic acid, kakuol, methyl eugenol, sesamin, and asarinin) in A. sieboldii were analyzed simultaneously using high-performance liquid chromatography (HPLC), and the proposed analytical method was validated. In addition, the inhibitory effects of the A. sieboldii extract and the 6 analyzed compounds on the expression of chemokine were evaluated in HaCaT cells. The HPLC method showed high linearity, with a correlation coefficient of ≥0.9999. The limits of detection for the 6 compounds ranged from 0.00 to 0.02 µg/mL, and the limits of quantification ranged from 0.01 to 0.05 µg/mL. The intraday and interday precisions were 0.15%-1.90%; the accuracy was 97.36%-103.23%, and the recoveries of the 6 compounds were 97.45%-103.93%. The content of each compound in A. sieboldii, as determined using the corresponding calibration curve, was in the range of 0.380-12.062 mg/g. This optimized simultaneous quantification method will be suitable for improving the quality control of A. sieboldii. Moreover, the 6 compounds in A. sieboldii showed an inhibitory effect on the production of chemokines, which suggests that A. sieboldii has an antiallergic effect.

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“…Volatile oils, lignans, and alkamides are considered to be the major constituents related to the pharmacological activity of ARR [ 3 , 4 , 5 , 6 ]. During the past few years, several studies analyzing the volatile oils, lignans, and alkamides in ARR by gas chromatography–mass spectrometry (GC–MS) [ 7 ], high–performance liquid chromatography (HPLC) [ 8 ], and ultra–performance liquid chromatography (UPLC) [ 9 , 10 ] have been reported, and these constituents can be used as marker compounds for the quality control of ARR.…”

Section: Introductionmentioning

confidence: 99%

The Relative Content and Distribution of Absorbed Volatile Organic Compounds in Rats Administered Asari Radix et Rhizoma Are Different between Powder- and Decoction-Treated Groups

Liu

Shang

et al. 2020

Molecules

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Asari Radix et Rhizoma (ARR) is an important traditional Chinese medicine. Volatile organic compounds (VOCs) are the main active constituents of ARR. Research on the metabolite profile of VOCs and the difference of absorbed constituents in vivo after an administration of ARR decoction and powder will be helpful to understand the pharmacological activity and safety of ARR. In this study, headspace solid-phase microextraction gas chromatography mass spectrometry (HS–SPME–GC–MS) was applied to profile the VOCs from ARR in rats in vivo. A total of 153 VOCs were tentatively identified; 101 were original constituents of ARR (98 in the powder-treated group and 43 in the decoction-treated group) and 15 were metabolites, and their metabolic reactions were mainly oxidation and reduction, with only two cases of methylation and esterification, and 37 unclassified compounds were identified only in the ARR-treated group. Of the 153 VOCs identified, 131 were reported in rats after oral administration of ARR for the first time, containing 79 original constituents, 15 metabolites, and 37 unclassified compounds. In the powder-treated group, methyleugenol, safrole, 3,5-dimethoxytoluene (3,5-DMT), 2,3,5-trimethoxytoluene (2,3,5-TMT), and 3,4,5-trimethoxytoluene (3,4,5-TMT) were the main absorbed constituents, the relative contents of which were significantly higher compared to the decoction-treated group, especially methyleugenol, safrole, and 3,5-DMT. In the decoction-treated group, 3,4,5-TMT, 2,3,5-TMT, kakuol, and eugenol were the main constituents with a higher content and wider distribution. The results of this study provide a reference for evaluating the efficacy and safety of ARR.

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Phytochemical, Toxicological and Pharmacological Studies of <i>Asiasari Radix</i> et Rhizoma: A Review

Cited by 17 publications

References 50 publications

Ethanol extract of asiasari radix preferentially induces apoptosis in G361 human melanoma cells by differential regulation of p53

Ethanol extract of asiasari radix preferentially induces apoptosis in G361 human melanoma cells by differential regulation of p53

Simultaneous Determination of 6 Antiallergic Components in Asarum sieboldii Using High-Performance Liquid Chromatography

The Relative Content and Distribution of Absorbed Volatile Organic Compounds in Rats Administered Asari Radix et Rhizoma Are Different between Powder- and Decoction-Treated Groups

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