Simultaneous Determination of 6 Antiallergic Components in Asarum sieboldii Using High-Performance Liquid Chromatography

Abstract

Owing to the side effects of current drugs for treating atopic dermatitis (AD), a chronic disease in the skin, traditional herbal medicine is receiving much attention as an alternative treatment. Asarum sieboldii has traditionally been used to treat colds, fevers, headaches, coughs, neuralgia, chronic bronchitis, asthma, and allergies. In this study, 6 compounds (echinacoside, vanillic acid, kakuol, methyl eugenol, sesamin, and asarinin) in A. sieboldii were analyzed simultaneously using high-performance liquid chromatography (HPLC), and the proposed analytical method was validated. In addition, the inhibitory effects of the A. sieboldii extract and the 6 analyzed compounds on the expression of chemokine were evaluated in HaCaT cells. The HPLC method showed high linearity, with a correlation coefficient of ≥0.9999. The limits of detection for the 6 compounds ranged from 0.00 to 0.02 µg/mL, and the limits of quantification ranged from 0.01 to 0.05 µg/mL. The intraday and interday precisions were 0.15%-1.90%; the accuracy was 97.36%-103.23%, and the recoveries of the 6 compounds were 97.45%-103.93%. The content of each compound in A. sieboldii, as determined using the corresponding calibration curve, was in the range of 0.380-12.062 mg/g. This optimized simultaneous quantification method will be suitable for improving the quality control of A. sieboldii. Moreover, the 6 compounds in A. sieboldii showed an inhibitory effect on the production of chemokines, which suggests that A. sieboldii has an antiallergic effect.

Higenamine in Plants as a Source of Unintentional Doping

“…There is evidence for the anti-inflammatory [ 134 ], anti-bacterial [ 135 ], anti-fungal [ 132 ], and anti-cancer activity [ 136 ] effects of Asarum sieboldii . Bioactive compounds of Asari radix, including methyl eugenol, sesamin, asarinin echinacoside, vanillic acid, and kakuol, are useful in treating allergies [ 137 , 138 , 139 ]. Xixin (Asari Radix et Rhizoma) can be radix and rhizoma from Asarum sieboldii or Asarum heterotropoides ( Asarum heterotropoides F. Schmidt) [ 140 ].…”

Synthesis and Bioactivities of Novel Piperonylic Acid Derivatives Containing a Sulfonic Acid Ester Moiety

“…Piperonylic acid is an aromatic acid mainly found in black pepper ( Moreira et al, 2021 ). Lots of research results revealed members of the piperonylic acid family had a range of biological activities and were further developed into a commercial drug and widely used in the field of medicine, such as oxolinic acid ( Yamazawa et al, 2021 ; Boycov et al, 2022 ; Quan et al, 2022 ), kakuol ( Jang et al, 2020 ; Matsumoto et al, 2020 ; Sui et al, 2020 ), and miloxacin ( Horie and Nakazawa, 1992 ; Ueno and Aoki, 1996 ; Ueno et al, 2001 ). In addition, piperonylic acid derivatives also showed good activity against bacteria ( Umadevi et al, 2013 ).…”

Imprinting Sensor Based on Honeycomb Graphene Oxide for the Determination of Echinacoside in Complex Samples

“…10 It is of great significance to strictly control the content of ECH in Chinese medicines and pharmaceutical preparations, as well as dynamic monitoring of drug concentrations in the body for its efficacy and the treatment of related diseases. 11 At present, the analysis methods of ECH are mainly based on chromatography, 12,13 which have been universally recognized but require complex sample preparation with poor portability, making on-site detection difficult and high detection cost.…”