Phytochemistry, Ethnopharmacology, Pharmacokinetics and Toxicology of Cnidium monnieri (L.) Cusson

Sun, Yue; Yang, Angela Wei Hong; Lenon, George Binh

doi:10.3390/ijms21031006

Cited by 42 publications

(36 citation statements)

References 96 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many of the inflammatory disease conditions such as rheumatoid arthritis, serum sickness, glomerulonephritis, and systemic lupus erythematosus are normally associated with denaturation of tissue proteins. In arthritis, for example, the denaturation of tissue proteins leads to the production of autoantigens [ 15 , 16 ]. Therefore, any agent that can prevent protein denaturation would be worth considering for anti-inflammatory drug development.…”

Section: Discussionmentioning

confidence: 99%

“…Bergapten (5-methoxypsoralen), a furocoumarin found in medicinal plants, has become a compound of considerable interest in current research [ 16 , 17 ]. Accumulated reports have demonstrated the potential therapeutic effects of bergapten in various disease conditions including psoriasis and vitiligo [ 18 , 19 ], Alzheimer's disease (AD) [ 20 ], depression [ 21 ], osteoporosis [ 22 ], cancer [ 23 ], colitis [ 24 ], and asthma [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Protective Effect of Bergapten against Human Erythrocyte Hemolysis and Protein Denaturation In Vitro

Aidoo

Konja

Henneh

et al. 2021

International Journal of Inflammation

View full text Add to dashboard Cite

Bergapten, a furocoumarin found in many medicinal plants, is used for the management of various conditions. The present in vitro study evaluated the ability of bergapten to prevent human erythrocyte hemolysis and protein denaturation. Bergapten administered at 10, 30, and 100 μg/ml exhibited a significant concentration-dependent protection on the erythrocyte membrane exposed to hypotonicity and heat-induced hemolysis. The concentration at which bergapten inhibited 50% of the cells from hemolysis (IC50) was determined on a dose-response curve, plotted as logarithmic (concentration) against percentage inhibition, keeping the hemolysis produced within the control group at 100%. Bergapten treatment produced an IC50 value of 7.71 ± 0.27 μg/ml and 4.23 ± 0.42 μg/ml for hypotonicity and heat-induced hemolysis, respectively. Diclofenac sodium at similar concentrations produced an IC50 value of 12.22 ± 0.30 μg/ml and 9.44 ± 0.23 μg/ml in the hypotonicity and heat-induced hemolysis, respectively. The ability of bergapten to inhibit protein denaturation was studied as part of an investigation on its mechanism of action. The results showed a significant concentration-dependent reduction in protein denaturation. When administered at 10, 30, and 100 μg/ml, bergapten produced a concentration-dependent reduction in albumin denaturation. Bergapten inhibited protein denaturation with IC50 values of 5.34 ± 0.30 μg/ml and 12.18 ± 0.20 μg/ml in the heat-treated egg albumin and bovine serum albumin denaturation experiments, respectively. Diclofenac sodium (10, 30, and 100 μg/ml) exhibited a similar protection against heat-treated egg albumin and bovine serum albumin denaturation experiments with IC50 values of 8.93 ± 0.17 μg/ml and 12.72 ± 0.11 μg/ml, respectively. Taken together, data from this study show that the pharmacological properties of bergapten may in part be related to its membrane-stabilizing and antidenaturation properties.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protective Effect of Bergapten against Human Erythrocyte Hemolysis and Protein Denaturation In Vitro

Aidoo

Konja

Henneh

et al. 2021

International Journal of Inflammation

View full text Add to dashboard Cite

show abstract

“…It was clear that filaggrin expression was significantly increased by the topical treatment of TCFC (Figure 8). Takamori et al, 2018). In addition, 20 and 80 mg/mL of TCTC notably suppressed the thymus index of rats, but only 80 mg/mL of TCTC exerted an inhibitory effect on the spleen index (Figure 5).…”

Section: Discussionmentioning

confidence: 90%

“…Until now, the coumarin compounds from C. monnieri have been confirmed to exert improved effects on allergic asthma and contact dermatitis (Chiang et al, 2017;Matsuda, Tomohiro, Ido, & Kubo, 2002). Only a few pharmacological studies of the effects of coumarins from C. monnieri on AD have been reported, no profound research on AD has been found to date (Sun, Yang, & Lenon, 2020).…”

mentioning

confidence: 99%

Ameliorative effects of total coumarins from the fructus of Cnidium monnieri (L.) Cuss. on 2,4‐dinitrochlorobenzene‐induced atopic dermatitis in rats

Zhang

Deng

Wang

et al. 2021

Phytotherapy Research

View full text Add to dashboard Cite

Atopic dermatitis (AD), which is characterized by intense pruritus and serious inflammation, is a chronic skin disease. Modern studies have testified that the total coumarins from the fructus of Cnidium monnieri (TCFC) possess evident biological activities based on their coumarin compounds. The purpose of this manuscript is to investigate the effects of topical use of TCFC on immune response, inflammation, and skin barrier function in rats with 2,4-dinitrochlorobenzene (DNCB)-induced AD. Results indicated that the skin lesion scores of rats were obviously reduced after the management of TCFC, and the spleen and thymus indices also were markedly repressed. TCFC significantly inhibited the overproduction of TNF-α, interferon-γ, interleukin (IL)-4, IL-13, thymic stromal lymphopoietin, and immunoglobulin E; the epidermal thickness and number of mast cells were notably decreased. The western blot experiment was conducted to determine the effects of TCFC on the mitogenactivated protein kinases signaling pathway. Results indicated that phosphorylation of extracellular signal-regulated kinases, p38, and c-Jun amino-terminal kinases was significantly blocked by TCFC. In addition, TCFC could upregulate the expression of filaggrin in dorsal skin, which means that TCFC showed a protective effect on skin barrier disruption. Furthermore, TCFC downregulated the levels of IL-1β, IL-4, IL-31, and TSLP mRNA and upregulated the expression of filaggrin mRNA in the dorsal skin of rats. Our research demonstrated the ameliorative effects of TCFC on AD-like rats by inhibiting immune response and inflammation and recovering skin barrier function.

show abstract

“…Cnidium monnieri (L.) Cusson (Chinese name: She chuang zi) is an important herbal medicine that has been used to treat chronic renal diseases, female genitals, male impotence, frigidity, and skin diseases for centuries in China ( Sun et al, 2020 ). It was documented that the biological activities of Cnidium monnieri (L.) Cusson are mainly mediated by its coumarin components, among which osthole has been studied extensively and is considered to play a vital role in the clinical efficacy of this herb ( Li et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Ameliorative Effects of Osthole on Experimental Renal Fibrosis in vivo and in vitro by Inhibiting IL-11/ERK1/2 Signaling

Zhao

Shao

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

Objectives: Natural product, osthole, has been proven to have a protective effect on organ fibrosis, including renal fibrosis. All of these studies are mainly focused on the regulation of TGF-β/Smad signaling pathway. However, due to the pleiotropic roles of TGF-β/Smad signaling, direct TGF-β-targeted treatments are unlikely to be therapeutically feasible in clinic. Recently, the downstream IL-11/ERK1/2 signaling of TGF-β has become an attractive therapeutic target without upstream disadvantages. Based on that, this study was designed to identify the potential effects of osthole on IL-11/ERK1/2 signaling pathway in renal fibrosis.Methods: The renal fibrosis model was established in vivo and in vitro, we investigated the effects of osthole on unilateral ureteral obstruction (UUO)-induced renal fibrosis and TGF-β-induced HK-2 cells. After preliminarily confirming the antifibrogenic effects of osthole and the link between its antifibrogenic effects and the inhibition of IL-11/ERK1/2 signaling, we applied a direct IL-11-induced HK-2 cells fibrosis model to further explore the inhibitory effects of osthole on IL-11/ERK1/2 signaling pathway.Results: Our results confirmed that osthole can decrease the secretion of fibrosis proteins, such as α-smooth muscle actin (α-SMA), collagen I, and fibronectin, ameliorate experimental renal fibrosis in vivo and in vitro, and the effect was associated with suppressing TGF-β1/Smad signaling. More importantly, we found that IL-11/ERK1/2 signaling in UUO-induced renal fibrosis and TGF-β-induced HK-2 cell model was obviously upregulated, and osthole treatment also significantly inhibited the abnormal IL-11/ERK1/2 signaling activation. Given the direct link between TGF-β/Smad signaling and IL-11/ERK1/2 signaling pathway, we have verified that osthole has a direct inhibitory effect on IL-11/ERK1/2 signaling independent of TGF-β signaling by using an IL-11-induced HK-2 cells fibrosis model. Osthole treatment decreased the protein expression of α-SMA, collagen I and fibronectin without changing their mRNA levels in IL-11-induced HK-2 cells. Moreover, it was observed that the IL-11/ERK1/2 inhibitor, U0126, partly blocked the antifibrogenic effects of osthole.Conclusion: In this study, we found that osthole has a previously unrecognized role in inhibiting IL-11/ERK1/2 signaling pathway. Our work demonstrated that the antifibrogenic effect of osthole is not only mediated by TGF-β/Smad2/3 signaling, but also directly mediated by IL-11/ERK1/2 signaling pathway independent of TGF-β1 signaling.

show abstract

Phytochemistry, Ethnopharmacology, Pharmacokinetics and Toxicology of Cnidium monnieri (L.) Cusson

Cited by 42 publications

References 96 publications

Protective Effect of Bergapten against Human Erythrocyte Hemolysis and Protein Denaturation In Vitro

Protective Effect of Bergapten against Human Erythrocyte Hemolysis and Protein Denaturation In Vitro

Ameliorative effects of total coumarins from the fructus of Cnidium monnieri (L.) Cuss. on 2,4‐dinitrochlorobenzene‐induced atopic dermatitis in rats

Ameliorative Effects of Osthole on Experimental Renal Fibrosis in vivo and in vitro by Inhibiting IL-11/ERK1/2 Signaling

Contact Info

Product

Resources

About