1976
DOI: 10.1104/pp.57.2.188
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Phytochrome Stability in Vitro

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1978
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Cited by 8 publications
(2 citation statements)
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“…Supernatant fractions were decanted through cheesecloth and pooled. Residual Ca^+ was determined and eliminated as described in Lisansky and Galston (1974). Seven to eight kg of tissue will yield 11 liters of extract with a A (AA)lm\ (5 cm path) of about 0.032.…”
Section: Extraction and Clarification Of Crude Extract From Etiolatedmentioning
confidence: 99%
See 1 more Smart Citation
“…Supernatant fractions were decanted through cheesecloth and pooled. Residual Ca^+ was determined and eliminated as described in Lisansky and Galston (1974). Seven to eight kg of tissue will yield 11 liters of extract with a A (AA)lm\ (5 cm path) of about 0.032.…”
Section: Extraction and Clarification Of Crude Extract From Etiolatedmentioning
confidence: 99%
“…We found that 5 mM Ca^"^ is sufficient to completely block phytochrome binding to brushite and that addition of 5 mM EDTA allowed 100% of the phytochrome in the same extract to bind to brushite. Therefore, all subsequent crude extracts were assayed for free Ca^+ (Lisansky and Galston 1974) which was then complexed with an equal molarity of EDTA.…”
Section: Effect On Phytochrome Binding To Calcium Phosphate Gelsmentioning
confidence: 99%