Phytochromes in Agrobacterium fabrum

Lamparter, Tilman; Xue, Peng; Elkurdi, Afaf; Kaeser, Gero; Sauthof, Luisa; Scheerer, Patrick; Krauß, Norbert

doi:10.3389/fpls.2021.642801

Cited by 12 publications

(14 citation statements)

References 92 publications

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“…The phytochrome superfamily acts as photoreceptors with biliverdin as their chromophores. In plants, bacteria, and fungi, they mediate a wide range of light responses, such as seed germination, floral induction, phototaxis, and photo-acclimation (1)(2)(3)(4)(5). Naturally occurring phytochromes are large, modular, and dimeric signaling proteins.…”

Section: Introductionmentioning

confidence: 99%

Light-induced protein structural dynamics in bacteriophytochrome revealed by time-resolved x-ray solution scattering

Lee

Kim

et al. 2022

Sci. Adv.

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Bacteriophytochromes (BphPs) are photoreceptors that regulate a wide range of biological mechanisms via red light–absorbing (Pr)–to–far-red light–absorbing (Pfr) reversible photoconversion. The structural dynamics underlying Pfr-to-Pr photoconversion in a liquid solution phase are not well understood. We used time-resolved x-ray solution scattering (TRXSS) to capture light-induced structural transitions in the bathy BphP photosensory module of Pseudomonas aeruginosa . Kinetic analysis of the TRXSS data identifies three distinct structural species, which are attributed to lumi-F, meta-F, and Pr, connected by time constants of 95 μs and 21 ms. Structural analysis based on molecular dynamics simulations shows that the light activation of PaBphP accompanies quaternary structural rearrangements from an “II”-framed close form of the Pfr state to an “O”-framed open form of the Pr state in terms of the helical backbones. This study provides mechanistic insights into how modular signaling proteins such as BphPs transmit structural signals over long distances and regulate their downstream biological responses.

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Section: Introductionmentioning

confidence: 99%

Light-induced protein structural dynamics in bacteriophytochrome revealed by time-resolved x-ray solution scattering

Lee

Kim

et al. 2022

Sci. Adv.

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“…We then tested if the triple mutant strain showed agp1 null mutant phenotype via a temperature‐sensitive growth assay. Agp1 / 2 are two phytochrome photoreceptor genes encoded in A. tumefaciens C58 genome which are responsible for sensing lights in blue, red, and far‐red wavelengths (Lamparter et al ., 2021). Previous studies in Agrobacterium have shown that Agp1/2 can inhibit cell propagation under nutrient deprivation (Xue et al ., 2021), as the single and double knockout mutants exhibited faster growth and reached higher cell densities than the WT strain during an expanded growth period.…”

Section: Resultsmentioning

confidence: 99%

CRISPR RNA‐guided integrase enables high‐efficiency targeted genome engineering in Agrobacterium tumefaciens

Aliu

Lee

Wang

2022

Plant Biotechnology Journal

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Summary Agrobacterium tumefaciens, the causal agent of plant crown gall disease, has been widely used to genetically transform many plant species. The inter‐kingdom gene transfer capability made Agrobacterium an essential tool and model system to study the mechanism of exporting and integrating a segment of bacterial DNA into the plant genome. However, many biological processes such as Agrobacterium‐host recognition and interaction are still elusive. To accelerate the understanding of this important plant pathogen and further improve its capacity in plant genetic engineering, we adopted a CRISPR RNA‐guided integrase system for Agrobacterium genome engineering. In this work, we demonstrate that INsertion of Transposable Elements by Guide RNA–Assisted TargEting (INTEGRATE) can efficiently generate DNA insertions to enable targeted gene knockouts. In addition, in conjunction with Cre‐loxP recombination system, we achieved precise deletions of large DNA fragments. This work provides new genetic engineering strategies for Agrobacterium species and their gene functional analyses.

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“…Phytochromes control a variety of physiological processes in plants and prokaryotes. [1][2][3][4][5] These photoreceptors are composed of a photosensory core module (PCM) carrying a tetrapyrrole chromophore, and an output module that is activated or deactivated via light-induced structural changes in the PCM. 6,7 Thus, phytochromes act as photoswitches with two parent states, the red-absorbing Pr and the far-red absorbing Pfr state in which the chromophore adopts a ZZZssa and ZZEssa configuration, respectively (Fig.…”

Section: Introductionmentioning

confidence: 99%

“…16 Only in a sub-group of bacterial phytochromes, the so-called bathy phytochromes, the order of stability is reversed such that Pfr is the stable dark state. 3,17 Although the various phytochromes differ with respect to the chemical constitution of the chromophore, the module composition, and -comparing plant and prokaryotic phytochromes -the type of functionally relevant protein structural changes, they share a common general reaction scheme of the PCM. 1,18 The photoinduced structural changes start with a Z/E photoisomerization of the methine bridge between rings C and D (Fig.…”

Section: Introductionmentioning

confidence: 99%