Phytoestrogens Enhance the Acetylcholinesterase Activity of the Rat Pheochromocytoma Cell Line PC12 by Binding to the Estrogen Receptor

Isoda, Hiroko; Ozawa, Tetsuo; Talorete, Terence P. N.; Seki, Mariko; Inamori, Yuhei

doi:10.1007/978-94-017-0726-8_73

Cited by 5 publications

(7 citation statements)

References 12 publications

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“…MYR decreases the toxicity of β-amyloid fibrils [7]. Genistein and daidzein increases AChE activity in rat neuronal cells PC12 [9]. (-)-Epigallocatechin gallate, hydroxytyrosol, resveratrol and tyrosol exhibit a cytoprotective effect on neuroblastoma IMR-32 and lymphoma U937 cell lines via an increase in the resistance of cellular DNA to oxidative damage [10].…”

Section: Introductionmentioning

confidence: 99%

Anticholinesterase activity of selected phenolic acids and flavonoids – interaction testing in model solutions

Szwajgier¹

2015

Ann Agric Environ Med.

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Szwajgier D. Anticholinesterase activity of selected phenolic acids and flavonoids: interaction testing in model solutions. Ann Agric Environ Med. 2015; 22(4): 690-694. doi: 10.5604/12321966.1185777 Abstract Introduction. Alzheimer's disease is a progressively developing neurodegenerative disorder of the central nervous system. The only present treatment of this disease is the use of acetyl-and butyrylcholinesterase inhibitors. Previously, the neuroprotection of phenolic acids and flavonoids in the brain has been indicated. Materials and method. This study measured anticholinesterase activities of 9 phenolic acids and 6 flavonoids, singly or in combination. The synergy/antagonism/zero interaction between compounds was evaluated taking into consideration the statistical significance. Ellman's modified spectrophotometric method was used with the simultaneous measurement of the false-positive effect of compounds. Results. The anti-acetylcholinesterase activity of phenolic acids was as follows: homogentisic acid > 4-hydroxyphenylpyruvic acid > nordihydroguaiaretic acid > rosmarinic acid > caffeic acid > gallic acid = chlorogenic acid > homovanillic acid > sinapic acid. p-Hydroxyphenylpyruvic, caffeic, chlorogenic, gentisic, homogentisic, nordihydroguaiaretic and rosmarinic acids in pairs exhibited, in most cases, a lower inhibitory activity (at p>0.05), than the sum of the activities of single compounds. Also, phenolic acids in pairs with flavonoids (cyanidin, delphinidin, kaempferol, myricetin, phloridzin, pelargonidin or quercetin) presented, in most cases, a lower inhibitory activity than could be calculated for both compounds singly (at p>0.05). Only in the case of a few samples was the inhibitory activity of two compounds higher than the sum of inhibitions exerted by the same compounds tested singly (either at p>0.05 or p<0.05). The lack of synergy of pairs of inhibitors suggests one small binding site, making impossible to accommodate both inhibitors adjacent to one another.

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Section: Introductionmentioning

confidence: 99%

Anticholinesterase activity of selected phenolic acids and flavonoids – interaction testing in model solutions

Szwajgier¹

2015

Ann Agric Environ Med.

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“…Genistein and daidzein (at 0.08-1.5 µmol/L) enhanced the AChE activity of the rat neuronal cell line PC12. Both polyphenols exhibited estrogenic activity in tests using breast cancer cell line MCF7 [Isoda et al, 2002]. It was shown that (-)-epigallocatechin gallate and hydroxytyrosol (at 10 µmol/L) exerted a cytoprotective effect on neuroblastoma IMR-32 and lymphoma U937 cell lines by the enhanced resistance of cellular DNA to oxidative damage.…”

Section: Introductionmentioning

confidence: 99%

Anticholinesterase Activities of Selected Polyphenols – a Short Report

Szwajgier¹

2014

Pol. J. Food Nutr. Sci.

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In this work, anticholinesterase activities of 24 polyphenolic compounds were tested using the modified Ellman's spectrophotometric method. The most efficient acetyl-and butyrylcholinesterase inhibitors were anthocyanins (pelargonidin, delphinidin and cyanidin), flavones (apigenin and luteolin), flavonols (quercetin, kaempferol and myricetin), as well as dihydrochalcone phloridzin and prenylated chalcone xanthohumol. It was established that all the tested compounds were within a narrow molecular weight range of 254.24-354.40 g/mol, which probably was not discriminative for their inhibitory activity. Among all the classes of polyphenolic compounds, the lowest activities were exerted by flavan-3-ols. The inhibitory activity of the tested polyphenols was decreased by the presence of a 3-hydroxyl group. A simultaneous substitution of a carbonyl group at position 4 and a hydroxyl group at position 3 or a lack of both of these substitutions had no effect on the activity of the investigated compounds. The number and position of other hydroxyl groups in the tested molecules played a minor role in this context. Aglycons were more effective cholinesterase inhibitors than their corresponding glycosylated forms. Overall, the results show that phenolic acids can play a role in neuroprotection. However, further in vitro and in vivo studies involving a larger number of polyphenolic compounds simultaneously with well-known cholinesterase inhibitors should be performed in the nearest future to confirm these findings. Unauthenticated Download Date | 5/8/18 4:25 AM

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“…It is suggested that this induction may due to the binding of estrogenic compounds to the estrogenic receptors. However, the study did not show AChE induction after E 2 exposure to PC12 cells of rats (Isoda et al 2002). The induction of AChE activity of carp after E 2 exposure may be attributed to the binding of E 2 to estrogenic receptors of the neural cells.…”

Section: Discussionmentioning

confidence: 94%

“…Our results supported these previous studies. The neurotransmitter acetylcholine binds to the acetylcholine (ACh) receptor, inducing a conformational change that opens this transmitter-gated ion channel (Isoda et al 2002). AChE hydrolyzes the ACh to end the cholinergic neural transmission in nervous systems of vertebrates, including fish (Arufe et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Water quality evaluation of two interconnected dam lakes with field-captured and laboratory-acclimated fish, Cyprinus carpio

Güngördü

Özmen

2011

Environ Monit Assess

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Karakaya and Sultansuyu Dam Lakes, located in the eastern part of Turkey, are important water sources, both for irrigation and fishery. The main goal of the study was to investigate water qualities of dam lakes using a set of biomarkers in the fish Cyprinus carpio. For this aim, field sample and laboratory-acclimated fish were compared to identify changes in selected biomarkers. The activities of ethoxyresorufin-O-deethylase, glutathione S-transferase, glutathione reductase, and carboxylesterase were determined in liver samples. Also, plasma and liver lactate dehydrogenase, aspartate aminotransferase, and alanine aminotransferase activities were assayed. Brain acetylcholinesterase and carboxylesterase activities were also determined. The hepatosomatic index and condition factors were calculated. Plasma vitellogenin assays were evaluated for the presence of xenoestrogen. Physicochemical values of water samples showed the existence of eutrophication risk, and also, some chemicals in both lakes were determined to be over tolerable limits. The comparisons of samples from both dam lake and laboratory-acclimated fish showed that the lakes may be at risk of pollution by some xenobiotics, namely xenoestrogens and acetylcholinesterase-inhibiting agents.

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Phytoestrogens Enhance the Acetylcholinesterase Activity of the Rat Pheochromocytoma Cell Line PC12 by Binding to the Estrogen Receptor

Cited by 5 publications

References 12 publications

Anticholinesterase activity of selected phenolic acids and flavonoids – interaction testing in model solutions

Anticholinesterase activity of selected phenolic acids and flavonoids – interaction testing in model solutions

Anticholinesterase Activities of Selected Polyphenols – a Short Report

Water quality evaluation of two interconnected dam lakes with field-captured and laboratory-acclimated fish, Cyprinus carpio

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