Phytosulphokine- , a peptidyl plant growth factor, stimulates somatic embryogenesis in carrot

Kobayashi, Toshihiro; Eun, Chang-Ho; Hanai, Hidetoshi; Matsubayashi, Yoshikatsu; Sakagami, Youji; Kamada, Hiroshi

doi:10.1093/jxb/50.336.1123

Cited by 44 publications

(45 citation statements)

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“…Since PSK is involved in nanomolar concentrations in the initial steps of cellular dedifferentiation, proliferation, and re-differentiation, its biological function is similar to that of plant hormones (Igasaki et al 2003;Matsubayashi et al 2004). PSK can not only induce cell division in suspension and protoplast cultures (Matsubayashi and Sakagami 1996;Matsubayashi et al 1997), but appears to play a role in diverse aspects of plant development and reproduction, including the formation of tracheary elements and adventitious roots (Yamakawa et al 1998;Matsubayashi et al 1999), somatic embryogenesis (Kobayashi et al 1999;Igasaki et al 2003), and pollen germination (Chen et al 2000). It seems that besides the widely used auxins and cytokinins, PSK can be a promising supplement for in vitro plant cultures, especially in the case of recalcitrant species.…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration from leaf-derived protoplasts within the Daucus genus: effect of different conditions in alginate embedding and phytosulfokine application

Maćkowska

Jarosz

Grzebelus

2014

Plant Cell Tiss Organ Cult

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Protoplasts of four Daucus carota subspecies and three wild Daucus species were isolated from 2-weekold shoot cultures during overnight incubation in an enzyme mixture composed of 1 % (w/v) cellulase Onozuka R-10 and 0.1 % (w/v) pectolyase Y-23. Before the culture, they were embedded in autoclave-or filter-sterilized alginate solution. Modified thin alginate layer (TAL) and extra thin alginate film (ETAF) techniques were applied for protoplast immobilization. A rich mineral-organic medium based on the formulation of Kao and Michayluk supplemented with 0.1 mg l -1 2,4-dichlorophenoxyacetic acid, 0.2 mg l -1 zeatin, and optionally 100 nM phytosulfokine (PSK), a peptidyl plant growth factor, was used for protoplast culture. Plating efficiency was genotype-dependent and in 40-day-old cultures, it varied from 10 % for Daucus pusillus to 73 % for D. carota subsp. sativus. A considerably higher ability in colony formation was observed in the modified TAL culture system using filter-sterilized alginate and in the presence of PSK in the protoplast culture medium. Plant regeneration through somatic embryogenesis stimulated by PSK application occurred for five out of the seven Daucus accessions used in the present study. We believe our data may facilitate the use of wild Daucus in somatic hybridization with cultivated carrot.

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Section: Introductionmentioning

confidence: 99%

Plant regeneration from leaf-derived protoplasts within the Daucus genus: effect of different conditions in alginate embedding and phytosulfokine application

Maćkowska

Jarosz

Grzebelus

2014

Plant Cell Tiss Organ Cult

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“…PSK induces dedifferentiation and callus growth of dispersed plant cells at low nanomolar concentrations, even at initial cell densities as low as ~300 cells/ml. Interestingly, it also stimulates tracheary element differentiation of Zinnia mesophyll cells without intervening cell division and stimulates somatic embryogenesis in carrot (Kobayashi et al, 1999). Such cellular dedifferentiation and re-differentiation, however, cannot be induced by PSK alone, but require in addition certain ratios and concentrations of auxin and cytokinin.…”

Section: Journal Of Cell Science 116 (19)mentioning

confidence: 99%

Ligand-receptor pairs in plant peptide signaling

Matsubayashi

2003

Journal of Cell Science

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Extensive studies on plant signaling molecules over the past decade indicate that plant cell-to-cell communication, as is the case with animal systems, makes use of small peptide signals and specific receptors. To date, four peptide-ligand-receptor pairs have been identified and shown to be involved in a variety of processes. Systemin and phytosulfokine (PSK), the first and second signaling peptides identified in plants, were isolated by biochemical purification based on their biological activities. Furthermore, their receptors have been biochemically purified from plasma membranes on the basis of specific ligand-receptor interactions. By contrast, the two other peptide signals, CLAVATA3 (CLV3) and the pollen S determinant SCR/SP11, were genetically identified during searches for specific ligands for receptors that had already been cloned. Systemin functions in the plant wound response, whereas PSK appears to cooperate with auxin and cytokinin to regulate cellular dedifferentiation and redifferentiation. CLV3 is important for meristem organization, binding to a heterodimeric receptor comprising the CLV1 and CLV2 proteins. SCR/SP11 instead plays a role in self-incompatibility, where it activates a signalling cascade that leads to rejection of pollen with the same S haplotype. These ligands all seem to bind to receptors that possess intrinsic kinase activity, and al least two of them are generated by proteolytic processing of larger precursor proteins.

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“…For example, arabinogalactan proteins (Kreuger and van Horst, 1993;Toonen et al, 1997) and phytosulfokine-a (Matsubayashi et al, personal communication; Kobayashi et al, 1999a). Thus, somatic embryogenesis in carrot is probably influenced by a balance between levels of stimulatory and inhibitory conditioning factors.…”

Section: Effects Of P-hydroxybenzylmentioning

confidence: 99%

Inhibitory Effects of p-Hydroxybenzyl Alcohol on Somatic Embryogenesis in Carrot Cell Cultures.

Kobayashi

Higashi

Kamada

2000

Plant Biotechnology

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Somatic embryogenesis in carrot (Daucus carooa L.) is strongly inhibited by certain factors that accumulate in the medium of cultures with high embryogenic carrot cell density. We previously identified p -hydroxybenzyl alcohol as one of these inhibitory factors. In this study, we performed a detailed analysis of the effects of p -hydroxybenzyl alcohol on somatic embryogenesis. p -Hydroxybenzyl alcohol strongly inhibited the formation of somatic embryos by suppressing cell division. The inhibitory effect on the development of globular embryos was stronger than that on the development of heart-shaped and torpedo -shaped embryos. In contrast, p -hydroxybenzyl alcohol had no effect on the proliferation of both undifferentiated embryogenic cells or non-embryogenic cells. These results indicate that p -hydroxybenzyl alcohol inhibits the division of cells in a way that is specific to the early stage of somatic embryogenesis and that plays an important role in the formation of somatic embryos.

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Phytosulphokine- , a peptidyl plant growth factor, stimulates somatic embryogenesis in carrot

Cited by 44 publications

References 0 publications

Plant regeneration from leaf-derived protoplasts within the Daucus genus: effect of different conditions in alginate embedding and phytosulfokine application

Plant regeneration from leaf-derived protoplasts within the Daucus genus: effect of different conditions in alginate embedding and phytosulfokine application

Ligand-receptor pairs in plant peptide signaling

Inhibitory Effects of p-Hydroxybenzyl Alcohol on Somatic Embryogenesis in Carrot Cell Cultures.

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