Medicinal and aromatic plants have the ability to transmit volatile allelochemicals and affect their surrounding organisms. In this regard, their interaction should also be considered. The inhibitory effects of 112 essential oils on lettuce seed and seedling were investigated by cotton swab method. Germination (G%), Mean germination time (MGT), Lethal of embryo (L%), dormancy (D%), radicle growth (R%), and hypocotyl growth (H%) were measured. Two methods were used for evaluating allelopathic interaction effects: the simplified modified dilution check-board technique (SMCT) and the isobologram. Thymus daenensis had the highest inhibitory effect on G% (IC50 = 2.9 ppm) and the most lethal effect on the embryo (LC50 = 7.2 ppm). Thymus transcaspicus, Dracocephalum moldavica, Artemisia sieberi and Amomum subulatum had the greatest effect on MGT. Ziziphora tenuior, Trachyspermum ammi and Pelargonium graveolens had the highest effect on D%. Origanum vulgare was the strongest growth inhibitor. The highest synergistic effect on G% was in A. subulatum + Mentha suaveolens, on H% was related to Perovskia abrotanoides + T. daenensis, and on R% was observed in Artemisia vulgaris + M. suaveolens. The results of this study can lead to identification of new phytotoxic compounds in EOs and control weeds more effectively.