Phytotoxicité de huit mycotoxines associées à la fusariose de l'épi chez le blé

Eudes, François; Comeau, A.; Rioux, S.; Collin, J.

doi:10.1080/07060660009500477

Cited by 60 publications

(47 citation statements)

References 13 publications

(12 reference statements)

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“…Eudes et al (2000) demonstrated the phytotoxicity of the six trichothecenes DON, 3-acetyldesoxynivalenol (3-ADON), NIV, DAS, T-2 toxin and HT2 in four wheat cultivars Norseman, Katepwa, Toropi and Nyu-Bay. They found that DON and 3-ADON were more toxic than T-2 toxin, HT-2 and DAS, and that those toxins inhibited wheat coleoptile elongation at very low concentrations.…”

Section: Gross Toxic Effects Of Trichothecenes On Animalsmentioning

confidence: 99%

Effects of trichothecene mycotoxins on eukaryotic cells: A review

Rocha

Ansari²,

Doohan³

2005

Food Additives and Contaminants

503

298

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The major products of the trichothecene mycotoxin biosynthetic pathway produced in a species-and sometimes isolatespecific manner by cereal-pathogenic Fusarium fungi include T-2 toxin, diacetoxyscirpenol, deoxynivalenol and nivalenol. This paper briefly reviews the major effects of such trichothecenes on the gross morphology, cytology and molecular signalling within eukaryotic cells. The gross toxic effects of select trichothecenes on animals include growth retardation, reduced ovarian function and reproductive disorders, immuno-compromization, feed refusal and vomiting. The phytotoxic effects of deoxynivalenol on plants can be summarized as growth retardation, inhibition of seedling and green plant regeneration. Trichothecenes are now recognized as having multiple inhibitory effects on eukaryote cells, including inhibition of protein, DNA and RNA synthesis, inhibition of mitochondrial function, effects on cell division and membrane effects. In animal cells, they induce apoptosis, a programmed cell death response. Current knowledge about the eukaryotic signal transduction cascades and downstream gene products activated by trichothecenes is limited, especially in plants. In mammalian cells, certain trichothecenes trigger a ribotoxic stress response and activate mitogen-activated protein kinases. DON mediates the inflammatory response by modulating the binding activities of specific transcription factors and subsequently inducing cytokine gene expression. Several genes are up-regulated in wheat in response to trichothecene mycotoxins; the significance, if any, of these genes in the host response to trichothecenes has yet to be elucidated.

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Section: Gross Toxic Effects Of Trichothecenes On Animalsmentioning

confidence: 99%

Effects of trichothecene mycotoxins on eukaryotic cells: A review

Rocha

Ansari²,

Doohan³

2005

Food Additives and Contaminants

503

298

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“…Previous studies using a DON-deficient G. zeae mutant showed that DON was responsible for reduced virulence by G. zeae toward wheat (29,33). However, the role of NIV in the virulence of G. zeae has not been quantitatively analyzed, although NIV is known to be less phytotoxic than DON (11). Combinations of isogenic strains differing only in trichothecene production, such as 88-1 and a transgenic 88-1 Tri13 deletion strain or H-11 and a transgenic H-11 strain carrying the 88-1 Tri13 gene, would be appropriate for these studies.…”

Section: Discussionmentioning

confidence: 99%

Tri13 and Tri7 Determine Deoxynivalenol- and Nivalenol-Producing Chemotypes of Gibberella zeae

Lee

Han

Kim

et al. 2002

Appl Environ Microbiol

279

199

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Gibberella zeae, a major cause of cereal scab, can be divided into two chemotypes based on production of the 8-ketotrichothecenes deoxynivalenol (DON) and nivalenol (NIV). We cloned and sequenced a Tri13 homolog from each chemotype. The Tri13 from a NIV chemotype strain (88-1) is located in the trichothecene gene cluster and carries an open reading frame similar to that of Fusarium sporotrichioides, whereas the Tri13 from a DON chemotype strain (H-11) carries several mutations. To confirm the roles of the Tri13 and Tri7 genes in trichothecene production by G. zeae, we genetically altered toxin production in 88-1 and H-11. In transgenic strains, the targeted deletion of Tri13 from the genome of 88-1 caused production of DON rather than NIV. Heterologous expression of the 88-1 Tri13 gene alone or in combination with the 88-1 Tri7 gene conferred on H-11 the ability to synthesize NIV; in the latter case, 4-acetylnivalenol (4-ANIV) also was produced. These results suggest that Tri13 and Tri7 are required for oxygenation and acetylation of the oxygen at C-4 during synthesis of NIV and 4-ANIV in G. zeae. These functional analyses of the Tri13 and Tri7 genes provide the first clear evidence for the genetic basis of the DON and NIV chemotypes in G. zeae.

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“…The first treatment consisted of a mixture of trichothecenes evaluated and reported in wheat coleoptiles (Eudes et al 2000…”

Section: Production Of Doubled Haploid Subpopulationsmentioning

confidence: 99%

“…(1 (McLean 1996;Wakulinski 1989;Eudes et al 2000). Synergistic effects of different trichothecenes have been reported, suggesting that mixtures of trichothecenes exert a stronger effect on the eukaryotic cells than would be expected from their additive effects (Jones et al 1995;Eudes et al 2001).…”

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confidence: 99%

Trichothecene-mediated in vitro selection in wheat for reduced mycotoxin accumulation caused by Fusarium graminearum

Eudes¹,

Comeau²,

Rioux³

et al. 2008

Can. J. Plant Sci.

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Eudes, F., Comeau, A., Rioux, S. and Collin, J. 2008. Trichothecene-mediated in vitro selection in wheat for reduced mycotoxin accumulation caused by Fusarium graminearum. Can. J. Plant Sci. 88: 1115Á1125. Trichothecene, a factor of aggressiveness of Fusarium graminearum in wheat fusarium head blight (FHB), was evaluated in an anther co-culture assay for the regeneration of doubled haploid (DH) lines with reduced mycotoxin accumulation. A Fusarium graminearum culture filtrate and a defined mixture of purified trichothecenes were compared with a control treatment in two F 1 -derived microspores populations. Frontana and Katepwa were the FHB resistant and intermediate resistant sources, respectively, and the cultivar Norseman was the FHB susceptible parent. A preliminary evaluation of the subpopulations of DH lines, using the point inoculation method in the greenhouse, showed selection effects for FHB resistance in the trichothecene co-cultured Frontana/Norseman subpopulation only. Three years of field evaluation using the spray inoculation method revealed that the DH subpopulation from the F 1 hybrid Frontana/Norseman co-culture in the presence of trichothecenes accumulated consistently less deoxynivalenol (DON) in the grain than the control subpopulation. The FHB symptoms were also significantly reduced for 1 yr (2001) in the same subpopulation. This subpopulation showed increased test weight, plant height and a 1.1-d delay in heading date when compared with the control subpopulation, under disease pressure. A trichothecene co-cultured DH subpopulation from Katepwa/Norseman also had a significantly lower DON content for 1 yr.Key words: Androgenesis, disease resistance, Gibberella zeae, mycotoxin, Triticum aestivum, wheat scab Eudes, F., Comeau, A., Rioux, S. et Collin, J. 2008. Se´lection in vitro avec des trichothe´ce`nes en vue d'une re´duction de la concentration de mycotoxine de Fusarium graminearum chez le ble´. Can. J. Plant Sci. 88: 1115Á1125. Les auteurs ont e´valueĺ es trichothe´ce`nes, facteur de l'agressivite´de Fusarium graminearum dans la fusariose de l'e´pi du ble´(FEB), lors d'une co-culture d'anthe`res en vue de la re´ge´ne´ration de ligne´es haploı¨des double´es (HD) qui accumuleraient moins de mycotoxines. Ils ont compare´un filtrat de Fusarium graminearum et un me´lange de´fini de trichothe´ce`nes purifie´es a`un traitement te´moin sur deux populations de microspores venant de la F 1 . Les varie´te´s Frontana et Katepwa e´taient les sources de re´sistance et de tole´rance a`la FEB, tandis que Norseman servait de parent sensible a`la maladie. Lorsqu'on utilise la me´thode d'inoculation ponctuelle en serre, l'e´valuation pre´liminaire des sous-populations DH re´ve`le que la se´lection pour la re´sistance a`la FEB n'affecte que la sous-population Frontana/Norseman cultive´e avec des trichothe´ce`nes. Trois anne´es d'e´valuation sur le terrain avec la technique d'inoculation par pulve´risation indiquent que la sous-population DH issue de la co-culture des hybrides de la F 1 Frontana/Norseman avec ...

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Phytotoxicité de huit mycotoxines associées à la fusariose de l'épi chez le blé

Cited by 60 publications

References 13 publications

Effects of trichothecene mycotoxins on eukaryotic cells: A review

Effects of trichothecene mycotoxins on eukaryotic cells: A review

Tri13 and Tri7 Determine Deoxynivalenol- and Nivalenol-Producing Chemotypes of Gibberella zeae

Trichothecene-mediated in vitro selection in wheat for reduced mycotoxin accumulation caused by Fusarium graminearum

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