Picrocrocin hydrolysis by immobilized ?-glucosidase

Comparative study of thermostabilty and ester synthesis ability of free and immobilized lipases on cross linked silica gel

“…The K m values of bacterial and fungal free lipases (1.41 and 0.3 mM, respectively) were found to be higher than that of the immobilized lipases (0.3 and 0.25 mM, respectively), so immobilization had increased the affinity of enzyme for the substrate. These results were in accordance with those obtained previously [37]. The V max values for both immobilized bacterial and fungal lipases (2.38 U/mg and 0.8 U/mg, respectively) were found to decrease slightly compared to their respective free lipases (2.65 U/mg and 0.85 U/mg, respectively).…”

Enzymes and food flavor‐A review

“…so it might be possible to produce natural aroma from agro-industrial residues. An example of this trend is the hydrolysis of picrocrocin, the glucoside precursor of safranal, the essential volatile characteristic of the saffron aroma and odor, by ß-glucosidase (Iborra et al 1992). The same type of process may be possible in the near future for the production of vanilla, by treatment of the precursor, the glucovanilline present in the pod with a ß-glucosidase.…”

Production Practices and Quality Assessment of Food Crops Volume 1

“…Methanol-water (Koyama et al, 1988;Tarantilis et al, 1995;Orfanou and Tsimidou, 1996;Straubinger et al, 1997;Lozano et al, 1999;Soeda et al, 2001), ethanolwater (Buchecker and Eugster, 1973;Speranza et al, 1984;Gómez et al, 1987b;Himeno and Sano, 1987;Visvanath et al, 1990;Oberdieck et al, 1991;Sujata et al, 1992;Loskutov et al, 2000) diethyl ether (Zarghami and Heinz, 1971;Pfander and Schurtenberger, 1982;Tarantilis and Polissiou, 1997;Zareena et al, 2001) petroleum ether (Tarantilis et al, 1994b) or aqueous extracts of saffron (Basker and Negbi, 1985;Alonso et al, 1990;Iborra et al, 1992a;Iborra et al, 1992b;Orfanou and Tsimidou, 1996;Tsimidou and Billiaderis, 1997;Selim et al, 2000) have been used. Methanol-water (Koyama et al, 1988;Tarantilis et al, 1995;Orfanou and Tsimidou, 1996;Straubinger et al, 1997;Lozano et al, 1999;Soeda et al, 2001), ethanolwater (Buchecker and Eugster, 1973;Speranza et al, 1984;Gómez et al, 1987b;Himeno and Sano, 1987;Visvanath et al, 1990;Oberdieck et al, 1991;…”

“…Finally, it was analysed by HPLC using three different conditions (see Table 3) and detected by UV-Vis. The main analytical methods that have been applied to the analysis of picrocrocin include thin layer chromatography (Iborra et al, 1992b;Sujata et al, 1992) and HPLC Tarantilis et al 1995;Alonso et al, 2001). Himeno and Sano (1987) reported the in vitro synthesis of picrocrocin in stigma like structures of saffron.…”

“…Except for its absorption maxima at 250 nm (broad band due to the α, β-unsaturated cycloaldehyde moiety), picrocrocin also exhibited a shoulder at 350 nm. Iborra et al (1992b) referred to the IR spectrum of an aqueous solution of picrocrocin in caesium chloride plates: 3400, 2950, 1620, 1410, 1360, 1080 and 1040 cm -1 in deutered water and 1410, 1350, 1075 and 1035 cm -1 in water. The main analytical methods that have been applied to the analysis of picrocrocin include thin layer chromatography (Iborra et al, 1992b;Sujata et al, 1992) and HPLC Tarantilis et al 1995;Alonso et al, 2001).…”