Pictorial keys for the identification of mosquitoes (Diptera: Culicidae) associated with Dengue Virus Transmission

Rueda, Leopoldo M.

doi:10.11646/zootaxa.589.1.1

Cited by 411 publications

(331 citation statements)

References 10 publications

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“…After collection, mosquitoes were frozen until dead or killed with chloroform, and then identified to species or lowest taxa group using published keys. [13][14][15] Mosquito DNA was extracted from the head and thorax using the 96-well format DNeasy Blood & Tissue Kit following manufacturer instructions for insect tissue (Qiagen Sciences Inc., Germantown, MD). Anopheles gambiae s.l.…”

Section: Methodsmentioning

confidence: 99%

Sampling Host-Seeking Anthropophilic Mosquito Vectors in West Africa: Comparisons of an Active Human-Baited Tent-Trap Against Gold Standard Methods

Krajacich¹,

Slade²,

Mulligan³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. In this study, we characterize the ability of the previously described Infoscitex tent (IST) to capture mosquitoes in comparison to either the Centers for Disease Control Light Trap hung next to individuals under a bed net (LTC) or to human landing catches (HLC). In Senegal, the IST caught 6.14 times the number of Anopheles gambiae sensu lato (s.l.), and 8.78 times the Culex group V mosquitoes as LTC. In one of two locations in Burkina Faso, the IST caught An. gambiae at a rate not significantly different than HLC. Of importance, 9.1-36.1% of HLC caught An. gambiae were blood fed, mostly with fresh blood, suggesting they fed upon the collector, whereas only 0.5-5.0% from the IST had partial or old blood. The IST also caught outdoor biting species in proportions comparable to HLC. The results show this tent provides a safer and effective alternative to the skill-dependent, risky, and laborious HLC method.

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Section: Methodsmentioning

confidence: 99%

Sampling Host-Seeking Anthropophilic Mosquito Vectors in West Africa: Comparisons of an Active Human-Baited Tent-Trap Against Gold Standard Methods

Krajacich¹,

Slade²,

Mulligan³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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“…Confirmation of species identification was performed with the taxonomic key of Rueda (2004). Adult females were kept in cages with 10% sucrose solution up to 24 h before blood was supplied.…”

Section: Obtaining the Mosquitoesmentioning

confidence: 99%

Detecting multiple DNA human profile from a mosquito blood meal

Rabêlo¹,

Albuquerque²,

Tavares³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Criminal traces commonly found at crime scenes may present mixtures from two or more individuals. The scene of the crime is important for the collection of various types of traces in order to find the perpetrator of the crime. Thus, we propose that hematophagous mosquitoes found at crime scenes can be used to perform genetic testing of human blood and aid in suspect investigation. The aim of the study was to obtain a single Aedes aegypti mosquito profile from a human DNA mixture containing genetic materials of four individuals. We also determined the effect of blood acquisition time by setting time intervals of 24, 48, and 72 h after the blood meal. STR loci and amelogenin were analyzed, and the results showed that human DNA profiles could be obtained from hematophagous mosquitos at 24 h following the blood meal. It is possible that hematophagous mosquitoes can be used as biological remains at the scene of the crime, and can be used to detect human DNA profiles of up to four individuals.

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“…Las larvas se colectaron utilizando un gotero, colocándose en frascos con alcohol al 70% etiquetado con los datos del lugar de colecta, fecha y tipo de depósito. Para la clasificación de las muestras de mosquitos se revisaron trabajos relacionados con la taxonomía y sistemá-tica de estos insectos (Hill & Hill 1948, Belkin et al 1970, Darsie 1994, Reinert 2000, Rueda 2004, Reinert et al 2004) pero se optó por la utilización de las claves dicotómicas morfológicas de culíci-dos de Cuba (González 2006), por la cercanía entre ambos países, similitud en la fauna de mosquitos y por carencia de claves para Haití.…”

Section: Muestreos Larvales En Sitios De Cría Naturalesunclassified

Contribución al conocimiento de la distribución y aspectos biológicos de algunas especies de mosquitos en Haití

Marquetti-Fernández¹,

Saint-Jean²,

Fuster-Callaba³

et al. 2013

analesbio

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El terremoto de 2010 en Haití agravó las condiciones higiénicas sanitarias y ambientales, al aumentar la disponibilidad de sitios de cría de mosquitos vectores. Ante esta situación, como parte de la ayuda que Cuba brinda a este país, se incluyó la vigilancia y lucha antivectorial como un componente importante en el control de las enfermedades vectoriales. En el presente estudio se realizó un muestreo larval de mosquitos en 35 comunas, distribuidas en los 10 departamentos de Haití en el período entre Junio 2010 -Junio, 2011, utilizándose la metodología recomendada por la OMS. Se identificaron 21 especies. Las más abundantes y de mayor distribución fueron Aedes aegypti, Culex quinquefasciatus, Anopheles albimanus y Culex nigripalpus, constituyendo el 90,1% del total. Durante este trabajo, se detectó la presencia de Aedes albopictus, que constituyó el primer reporte de esta especie para Haití. Estos resultados actualizados constituyen un aporte al conocimiento de la fauna de mosquito en Haití.Palabras clave: Culicidae, Mosquitos, Distribución, Sitios de cría, Haití Abstract Contribution to knowledge of distribution of mosquitoes and some biological aspects of mosquitoes in HaitiThe earthquake occurred in Haiti in 2010 led to a worsening of health and environmental sanitation, since the availability of breeding sites for mosquito vectors were increased. In this situation, as part of aid that Cuba offers to this country, a rapid field assessment of entomological surveillance, was included as an important component in the control of vector-borne diseases. The larval sampling was conducted in 35 districts distributed over 10 departments of Haiti in the period June 2010 -June, 2011, using the methods recommended by the WHO. 21 species of mosquito were identified. The most abundant and widely distributed species were Aedes aegypti, Culex quinquefasciatus, Culex nigripalpus and Anopheles albimanus, accounting for 90.1% of total samples. During the realization of this entomological survey was carried out the first record of Aedes albopictus in Haiti. These updated results are a contribution to the knowledge of the mosquitoes fauna in Haiti.

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Pictorial keys for the identification of mosquitoes (Diptera: Culicidae) associated with Dengue Virus Transmission

Cited by 411 publications

References 10 publications

Sampling Host-Seeking Anthropophilic Mosquito Vectors in West Africa: Comparisons of an Active Human-Baited Tent-Trap Against Gold Standard Methods

Sampling Host-Seeking Anthropophilic Mosquito Vectors in West Africa: Comparisons of an Active Human-Baited Tent-Trap Against Gold Standard Methods

Detecting multiple DNA human profile from a mosquito blood meal

Contribución al conocimiento de la distribución y aspectos biológicos de algunas especies de mosquitos en Haití

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