Piezoelectric immunosensor for the direct and rapid detection ofFrancisella tularensis

Pohanka, Miroslav; Skládal, Petr

doi:10.1007/bf02932086

Cited by 31 publications

(15 citation statements)

References 20 publications

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“…The present study confirms the applicability of ELISA method for mass processing of bacterial isolates. Even limit of detection (LOD) was approximately on the similar level as in the case of performance of biosensors for F. tularenis 27 or E. coli 28 . Though the mobile laboratories and reconnaissance teams are typically equipped with portable PCR (such as R.A.P.I.D.…”

Section: Indirect Elisa For the Detection Of F Tularensismentioning

confidence: 71%

ELISA Detection of Francisella tularensis using Polyclonaland Monoclonal Antibodies

Pohanka¹,

Pavliš²,

Kroča³

2008

DSJ

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The mouse monoclonal and polyclonal antibodies were produced for the detection of intracellular pathogen and potential warfare agent Francisella tularensis. Antibody titers obtained were 1:640 for polyclonal antibodies and 1:320 for monoclonal antibodies. Both antibodies were used in the indirect enzyme-linked immunosorbent assay (ELISA) found to detect F. tularensis whole cells. The limit of detection was 5.4×106 CFU/ml for polyclonal antibodies and 6.9×106 CFU/ml for monoclonal antibodies. The value sample could be distinguished from any concentration of another gram-negative bacterium: Escherichia coli.

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Section: Indirect Elisa For the Detection Of F Tularensismentioning

confidence: 71%

ELISA Detection of Francisella tularensis using Polyclonaland Monoclonal Antibodies

Pohanka¹,

Pavliš²,

Kroča³

2008

DSJ

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“…Biosensors have been developed for characterization of antibodies [22, 23, 24] and several microorganisms [25] such as the biological agent Francisella tularensis [26] or model Escherichia coli [27] in real time; similar biosensors could even be applied for aflatoxin assays [28]. The last mentioned could act as an interfering compound; however, these compounds are quite hydrophobic and less likely to be found than organophosphates.…”

Section: Resultsmentioning

confidence: 99%

Amperometric Biosensors for Real Time Assays of Organophosphates

Pohanka

Jun

Kuča

2008

Sensors

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An amperometric biosensor based on acetylcholinesterase (AChE) immobilized in gelatin was used to develop an assay for the organophosphate paraoxon. The more traditional manner employing preincubation was used for comparison between measurement procedures, although the aim of the study was to examine the performance of the biosensor for real time monitoring of organophosphates. The biosensor was immersed in a reaction chamber and paraoxon was injected inside. We were able to detect 200 pg of paraoxon within one minute or 2.5 ppb when the biosensor was preincubed in the sample solution for 15 minutes. The practical impact and expectations are discussed.

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“…cells able to initiate the infection in humans this microbe is enrolled in the list of biological warfare agents (Pohanka and Skládal 2009) and new rapid methods of detection have recently been developed (Pohanka and Skládal 2007). Vol.…”

Section: Abbreviationsmentioning

confidence: 99%

J774 macrophage-like cell line cytokine and chemokine patterns are modulated by Francisella tularensis LVS strain infection

et al. 2010

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Mutual interactions were investigated between intracellular parasitic bacterium Francisella tularensis (F.t.; highly virulent bacterium responsible for tularemia, replicating within the host macrophages) and murine macrophage-like cell line J774. Recombinant murine lymphokine INF-gamma and/or LPS derived from E. coli were determined to stimulate in vitro antimicrobial activity of macrophage-like J774 cell line against the live vaccine strain (LVS) of F.t. through their ability to produce proinflammatory cytokines and chemokines. F.t. infection up-regulated IL-12 p40 production and down-regulated TNF-alpha production by stimulated macrophages; on the other hand, F.t. infection did not affect the production of IL-8, IL-6, MCP-5, and RANTES by stimulated macrophages. This showed that F.t. infection modulates the cytokine synthesis by J774 macrophage cell line.

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Piezoelectric immunosensor for the direct and rapid detection ofFrancisella tularensis

Cited by 31 publications

References 20 publications

ELISA Detection of Francisella tularensis using Polyclonaland Monoclonal Antibodies

ELISA Detection of Francisella tularensis using Polyclonaland Monoclonal Antibodies

Amperometric Biosensors for Real Time Assays of Organophosphates

J774 macrophage-like cell line cytokine and chemokine patterns are modulated by Francisella tularensis LVS strain infection

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