Piezoelectric nanocomposite bioink and ultrasound stimulation modulate early skeletal myogenesis

Paci, Claudia; Iberite, Federica; Arrico, Lorenzo; Vannozzi, Lorenzo; Parlanti, Paola; Gemmi, Mauro; Ricotti, Leonardo

doi:10.1039/d1bm01853a

Cited by 20 publications

(21 citation statements)

References 103 publications

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“…Although no dense population of surrounding inflammatory‐like cells was detected, an increased number of infiltrating cells was found compared to the earlier time points, whereby a round‐like nucleus, rather than cytoplasm‐surrounded, configuration was again visible, in agreement with a previous study. [ 37 ]…”

Section: Resultsmentioning

confidence: 99%

“…Although no dense population of surrounding inflammatory-like cells was detected, an increased number of infiltrating cells was found compared to the earlier time points, whereby a round-like nucleus, rather than cytoplasm-surrounded, configuration was again visible, in agreement with a previous study. [37] The thiol-ene hydrogels presented in this work were designed to mimic skeletal muscle native elasticity, and, ultimately, to support tissue repair and encourage biomaterial integration. While Mucograft presented a lower immune response and reduced inflammation, this investigation in vivo indicated a low dimensional variation in sample CollPEG4.5 after 7 d, in line with the previously measured high gel content (Figure 3) and degradation profile in vitro (Figure 6).…”

Section: Subcutaneous Study In Ratsmentioning

confidence: 99%

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Retracted: A Photoclick Thiol‐Ene Collagen‐Based Hydrogel Platform for Skeletal Muscle Tissue Engineering

Holmes

Yang

Wood

et al. 2023

Macro Materials & Eng

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UV‐cured collagen‐based hydrogels hold promise in skeletal muscle regeneration due to their soft elastic properties and porous architecture. However, the complex triple helix conformation of collagen and environmental conditions, i.e., molecular oxygen, pose risks to reaction controllability, wet‐state integrity, and reproducibility. To address this challenge, a photoclick hydrogel platform is presented through an oxygen‐insensitive thiol‐ene reaction between 2‐iminothiolane (2IT)‐functionalized type I collagen and multiarm, nonhomopolymerizable norbornene‐terminated polyethylene glycol (PEG). UV‐induced network formation is demonstrated by oscillatory time sweeps on the reacting thiol‐ene mixture, so that significantly increased storage moduli are measured and adjusted depending on the photoinitiator concentration. Variations in PEG functionality (4‐arm and 8‐arm) and PEG content generate hydrogels with skeletal muscle native stiffness (Ec = 1.3 ± 0.2‒11.5 ± 0.9 kPa), diffusion‐controlled swelling behavior and erosion‐driven degradability. In vitro, no cytotoxic effect is detected on C2C12 murine myoblasts, while myogenic differentiation is successfully accomplished on hydrogel‐seeded cells in then low serum culture medium. In vivo, 7 d subcutaneous implantation of selected thiol‐ene hydrogel in rats reveal higher cell infiltration, blood vessel formation, and denser tissue interface compared to a clinical gold standard collagen matrix (Mucograft, a trademark of Geistlich Biomaterials). These results, therefore, support the applicability and further development of this hydrogel platform for skeletal muscle regeneration.

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Section: Resultsmentioning

confidence: 99%

Section: Subcutaneous Study In Ratsmentioning

confidence: 99%

Retracted: A Photoclick Thiol‐Ene Collagen‐Based Hydrogel Platform for Skeletal Muscle Tissue Engineering

Holmes

Yang

Wood

et al. 2023

Macro Materials & Eng

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“…Experiments showed that there are no adverse effects on the biological activity of cells at a nanoparticle concentration of 250 µg/mL. Overexpression of MYOD1, MYOG, and MYH2 genes occurred when samples were stimulated by ultrasound for three continuous days during differentiation, which indicates ultrasonically stimulated barium titanate nanoparticles can be utilized to promote myoblast differentiation in 3D bioprinted configurations [ 105 ]. Zhu et al reported an energy harvester for tumor (4T1 breast cancer) therapy by embedding tetragonal BaTiO 3 nanoparticles in a thermosensitive hydrogel (chitosan hydrogel), which can generate toxic hydroxyl and superoxide radicals under the stimulation of ultrasound.…”

Section: Hydrogel-based Piezoelectric Ultrasonic Devicesmentioning

confidence: 99%

Recent Progress on Hydrogel-Based Piezoelectric Devices for Biomedical Applications

Lin

et al. 2023

Micromachines

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Flexible electronics have great potential in the application of wearable and implantable devices. Through suitable chemical alteration, hydrogels, which are three-dimensional polymeric networks, demonstrate amazing stretchability and flexibility. Hydrogel-based electronics have been widely used in wearable sensing devices because of their biomimetic structure, biocompatibility, and stimuli-responsive electrical properties. Recently, hydrogel-based piezoelectric devices have attracted intensive attention because of the combination of their unique piezoelectric performance and conductive hydrogel configuration. This mini review is to give a summary of this exciting topic with a new insight into the design and strategy of hydrogel-based piezoelectric devices. We first briefly review the representative synthesis methods and strategies of hydrogels. Subsequently, this review provides several promising biomedical applications, such as bio-signal sensing, energy harvesting, wound healing, and ultrasonic stimulation. In the end, we also provide a personal perspective on the future strategies and address the remaining challenges on hydrogel-based piezoelectric electronics.

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“…Also, affects cell behavior, for example in signaling and protein expression [72,73]. Thus, to prevent adverse cell responses and bioprinting-related death, shear stress must be considered [74,75]. Following extrusion, the shear rate drops with the corresponding climb in viscosity.…”

Section: Inks' Physicochemical/rheology Proprietiesmentioning

confidence: 99%

Biomaterials of human source for 3D printing strategies

et al. 2023

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Three-dimensional printing has risen in recent years as a promising approach that fast-tracked the biofabrication of tissue engineering constructs that most resemble utopian tissue/organ replacements for precision medicine. Additionally, by using human-sourced biomaterials engineered towards optimal rheological proprieties of extrudable inks, the best possible scaffolds can be created. These can encompass native structure and function with a low risk of rejection, enhancing overall clinical outcomes; and even be further optimized by engaging in information- and computer-driven design workflows. This paper provides an overview of the current efforts in achieving ink’s necessary rheological and print performance proprieties towards biofabrication from human-derived biomaterials. The most notable step for arranging such characteristics to make biomaterials inks are the employed crosslinking strategies, for which examples are discussed. Lastly, this paper illuminates the state-of-the-art of the most recent literature on already used human-sourced inks; with a final emphasis on future perspectives on the field.

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Piezoelectric nanocomposite bioink and ultrasound stimulation modulate early skeletal myogenesis

Abstract: A cell-laden alginate/Pluronic-based bioink doped with BaTiO3 piezoelectric nanoparticles (BTNPs) was investigated. BTNPs promoted myogenic differentiation and the synergy with ultrasound boosted the expression of MYOD1, MYOG, and MYH2 genes.

Cited by 20 publications

References 103 publications

Retracted: A Photoclick Thiol‐Ene Collagen‐Based Hydrogel Platform for Skeletal Muscle Tissue Engineering

Retracted: A Photoclick Thiol‐Ene Collagen‐Based Hydrogel Platform for Skeletal Muscle Tissue Engineering

Recent Progress on Hydrogel-Based Piezoelectric Devices for Biomedical Applications

Biomaterials of human source for 3D printing strategies

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