PIGF and Flt-1 on the surface of macrophages induces the production of TGF-β1 by polarized tumor-associated macrophages to promote lung cancer angiogenesis

Kong, Xianglong; Bu, Jianlong; Chen, Junhui; Ni, Bo-Xiong; Fu, Bicheng; Zhou, Fucheng; Pang, Sainan; Zhang, Jian; Xu, Shidong; He, Chuanchao

doi:10.1016/j.ejphar.2021.174550

Cited by 19 publications

(10 citation statements)

References 22 publications

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“…Macrophages play a pivotal role in the immune response and exert proinflammatory or anti‐inflammatory functions through phenotype polarization 34 . On the 7th day postoperation, IF staining was performed to evaluate the expression of F4/80, a macrophage marker, 35 and nitric oxide synthase‐2 (NOS2), a proinflammatory phenotype marker, 36 in the peritendinous tissue. The images in Figure 5B showed that after surgery, a large number of F4/80+ cells aggregated in the peritendinous tissue, and the treatment of the PCL membrane upregulated the expression of NOS2 in these cells.…”

Section: Resultsmentioning

confidence: 99%

A unidirectional drug‐release Janus membrane based on hydrogen bonding barrier effect for preventing postoperative adhesion and promoting tissue repair

Wang,

He,

Zhao

et al. 2023

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Barrier membranes incorporated with anti‐adhesion drugs have been widely used for preventing postoperative adhesion. However, the bidirectional release of drugs may interfere with internal tissue healing. Herein, a unidirectional Janus membrane composed of two functional layers is prepared by sequential electrospinning. The outer layer is designed as random polycaprolactone fibers incorporated with tannic acid/Fe3+ particles to prevent tissue adhesion, while the inner layer is designed as oriented gelatin fibers to promote tissue repair. Hydrogen bonding was employed to act as a barrier for preventing the diffusion of the tannic acid to the inner side, and thus the release of tannic acid occurs in a unidirectional manner. As a result, asymmetric biological performances of the Janus membrane are achieved. The outer face can provide anti‐inflammatory capability to the membrane and inhibit the viability of fibroblasts, while the inner face can promote the proliferation and differentiation of tendon stem cells. Tendon injured model confirms the prominent inflammation modulation, adhesion prevention, and repair promotion capabilities of the Janus membrane in vivo. This study provides a new strategy to develop advanced functional barrier membranes for postoperative adhesion prevention and internal tissue repair promotion.

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Section: Resultsmentioning

confidence: 99%

A unidirectional drug‐release Janus membrane based on hydrogen bonding barrier effect for preventing postoperative adhesion and promoting tissue repair

Wang,

He,

Zhao

et al. 2023

VIEW

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“…To determine the cytokines through which M2-TAMs affect the function of LUAD cells, we used a Luminex liquid-chip to analyze the content of various cytokines in the supernatant of the M2-TAMs co-culture group and the control group, and the results showed the contents of M2 markers IL - 10 , TGF - β1 , and CCL3 were significantly increased in the supernatant of co-culture group ( Figure 5B ). IL - 10 and TGF - β1 are both well-known anti-inflammatory cytokines, and M2-TAMs-derived TGF - β1 has been shown to promote lung cancer angiogenesis ( 19 ), liver cancer, and colorectal cancer migration and invasion ( 20 ). CCL3 is closely related to immune surveillance and tolerance and has become a prognostic biomarker for a variety of malignant tumors ( 21 ).…”

Section: Resultsmentioning

confidence: 99%

M2-TAMs promote immunoresistance in lung adenocarcinoma by enhancing METTL3-mediated m6A methylation

Wu¹,

Cheng²,

Yang³

et al. 2022

Ann Transl Med

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Background: Immunotherapy has become the first-line treatment for advanced non-small-cell lung cancer (NSCLC), but most patients still fail to benefit or have disease progression following treatment.M2 phenotype tumor-associated macrophages (M2-TAMs) are important cellular components in the immunosuppressive microenvironment of NSCLC, but how they contribute to immunoresistance remains unclear. This study was conducted to investigate the role and mechanism of M2-TAMs in NSCLC immunoresistance.Methods: We collected postoperative tumor samples for detection of M2-TAMs and other immune cells infiltration by immunofluorescence detection and flow cytometry. We then constructed a non-contact cell co-culture system using Transwell chambers. CCK-8, colony formation, wound healing and invasion assays were performed to evaluated the effect of M2-TAMs on the proliferation, migration and invasion abilities of lung adenocarcinoma (LUAD) cells in vitro. Xenograft model were performed to analysis the effect of M2-TAMs on the tumorigenesis and metastasis of LUAD cells in vivo.Results: M2-TAMs were greatly increased in the tumor tissue of patients with immunoresistant LUAD.They could significantly promote the proliferation, invasion, and migration of LUAD cells, and improve their resistance to cytotoxic T lymphocytes (CTL) cytotoxicity. Further research showed M2-TAMs could considerably enhance the expression of METTL3 and total m6A RNA level in LUAD cells and interfering with METTL3 could significantly reverse the impairment of M2-TAMs on the efficacy of CTL in killing tumor cells. Conclusions:In conclusion, M2-TAMs could promote LUAD immunoresistance by enhancing METTL3-mediated m6A methylation. Our results suggest METTL3 could be a potential therapeutic target for reversing immunoresistance and shed new light on the mechanism of M2-TAMs promoting LUAD immunoresistance.

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“…This indicates that KIFC1 may play a part in regulating the polarization of TAM. Researchers have found that TAM can promote tumor cells’ growth and metastasis through a variety of pathways ( Batoon and McCauley, 2021 ; Kong et al, 2021 ). This could partly explain why KIFC1 can promote LIHC progress.…”

Section: Discussionmentioning

confidence: 99%

Prognostic Value and Immunological Role of KIFC1 in Hepatocellular Carcinoma

Han

et al. 2022

Front. Mol. Biosci.

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As one of the members of the kinesin family, the role and potential mechanism of kinesin family member C1 (KIFC1) in the development of liver hepatocellular carcinoma (LIHC), especially in the immune infiltration, have not been fully elucidated. In this study, multiple databases and immunohistochemistry were employed to analyze the role and molecular mechanism including the immune infiltration of KIFC1 in LIHC. Generally, KIFC1 mRNA expression was overexpressed in LIHC tissues than normal tissues, and its protein was also highly expressed in the LIHC. KIFC1 mRNA expression was correlated with tumor grade and TNM staging, which was negatively correlated with overall survival and disease-free survival. Moreover, univariable and multivariate Cox analysis revealed that upregulated KIFC1 mRNA is an independent prognostic factor for LIHC. The KIFC1 promoter methylation level was negatively associated with KIFC1 mRNA expression and advanced stages and grade in LIHC. The different methylation sites of KIFC1 had a different effect on the prognosis of LIHC. Specifically, the KIFC1 mRNA expression level showed intense correlation with tumor immunity, such as tumor-infiltrating immune cells and immune scores as well as multiple immune-related genes. Moreover, KIFC1 co-expressed with some immune checkpoints and related to the responses to immune checkpoint blockade (ICB) and chemotherapies. Significant GO analysis showed that genes correlated with KIFC1 served as catalytic activity, acting on DNA, tubulin binding, histone binding, ATPase activity, and protein serine/threonine kinase activity. KEGG pathway analysis showed that these genes related to KIFC1 are mainly enriched in signal pathways such as cell cycle, spliceosome, pyrimidine metabolism, and RNA transport. Conclusively, KIFC1 was upregulated and displayed a prognostic value in LIHC. Moreover, KIFC1 may be involved in the LIHC progression partially through immune evasion and serve as a predictor of ICB therapies and chemotherapies.

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PIGF and Flt-1 on the surface of macrophages induces the production of TGF-β1 by polarized tumor-associated macrophages to promote lung cancer angiogenesis

Cited by 19 publications

References 22 publications

A unidirectional drug‐release Janus membrane based on hydrogen bonding barrier effect for preventing postoperative adhesion and promoting tissue repair

A unidirectional drug‐release Janus membrane based on hydrogen bonding barrier effect for preventing postoperative adhesion and promoting tissue repair

M2-TAMs promote immunoresistance in lung adenocarcinoma by enhancing METTL3-mediated m6A methylation

Prognostic Value and Immunological Role of KIFC1 in Hepatocellular Carcinoma

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