Piglets produced from in vivo blastocysts vitrified using the Cryologic Vitrification Method (solid surface vitrification) and a sealed storage container

Beebe, L.F.S.; Bouwman, E.G.; McIlfatrick, Stephen M.; Nottle, Mark B.

doi:10.1016/j.theriogenology.2010.11.043

Cited by 35 publications

(27 citation statements)

References 18 publications

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“…A recent report in porcine [65] describes the birth of piglets after transfer of in vivo-produced porcine em- No differences were found. N1, embryos processed for PCR; N2, embryos with the expected sex.…”

Section: Discussionmentioning

confidence: 97%

In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm

et al. 2012

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In this work we analyzed the effects of three culture systems on developmental ability of bovine embryos in vitro produced with sexed sperm, the survival to vitrification (cryologic vitrification method) of such blastocysts, and their pregnancy rates after embryo transfer to recipients, both as fresh and after vitrification/warming. Finally, we measured the accuracy of the sorting protocol by a polymerase chain reaction-based method to validate the embryo sex at blastocyst stages. We confirmed an individual effect of the bull as well as development rates of embryos produced with sorted sperm lower than embryos with unsorted sperm, independent of the culture system used. The cryoresistance to vitrification of embryos produced with sexed sperm did not differ from that of conventionally produced embryos (re-expansion rates at 24 and 48 h: 74.6% vs. 75.5%, and 64.5% vs. 68.1% for embryos produced with conventional and sorted sperm, respectively; hatching rates at 48 h: 63.55% vs. 55.5% for embryos produced with conventional and sorted sperm, respectively). Finally, no significant differences were found in pregnancy rates after the embryo transfer of fresh and vitrified/warmed blastocysts (52.8% vs. 42.0%, respectively; P Ͼ 0.05). Male and female embryos produced with sorted sperm showed the same quality in terms of developmental ability, cryoresistance, and pregnancy rates after transfer. Our culture system, coupled with the vitrification in fiber plugs, provides good quality sex-known embryos which survive vitrification at similar rates than embryos produced with conventional unsorted sperm; also it produces good pregnancy rates after transfer of sexed embryos both fresh and after vitrification and warming.

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Section: Discussionmentioning

confidence: 97%

In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm

et al. 2012

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“…However, these devices allow direct contact of the medium containing the embryos with liquid nitrogen, which expose the embryos to potential risk of microbial contamination. To avoid contamination of pathogens, closed systems have also been successfully developed to vitrify in vivo-derived porcine embryos [26].…”

Section: Long-term Storage Of Embryosmentioning

confidence: 99%

“…The sensitivity of porcine embryos to cryopreservation has been ascribed to their high lipid content in the cytoplasm [9,30], which decreases as the development stage increases. The successful vitrification of porcine embryos has, to date, been limited to the morula and blastocyst stages [24,26,[31][32][33][34]. Effective methods for the cryopreservation of earlier embryos (zygotes and two-to four-cell embryos) could be valuable for genetic rescue in sanitary crises that involve the extermination of all animals, where it is not possible to choose the collection day to obtain the most suitable embryos for cryopreservation, and for the development of new biotechnologies, such as cloning programs [35][36][37].…”

Section: Long-term Storage Of Embryosmentioning

confidence: 99%

Recent advances toward the practical application of embryo transfer in pigs

et al. 2016

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“…In essence this separates the cryogenic fluid from the samples by using the cryogen to cool a sterile solid surface [144] onto which the microdroplets of vitrification solution containing the cells or tissue are dropped. This method has now been commercialised and used to vitrify murine ovarian tissue and porcine blastocysts [145,146]. …”

Section: Cryopreservation By Conventional Slow Coolingmentioning

confidence: 99%

Cryopreservation of Human Stem Cells for Clinical Application: A Review

Hunt¹

2011

Transfus Med Hemother

280

207

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Stem cells have been used in a clinical setting for many years. Haematopoietic stem cells have been used for the treatment of both haematological and non-haematological disease; while more recently mesenchymal stem cells derived from bone marrow have been the subject of both laboratory and early clinical studies. Whilst these cells show both multipotency and expansion potential, they nonetheless do not form stable cell lines in culture which is likely to limit the breadth of their application in the field of regenerative medicine. Human embryonic stem cells are pluripotent cells, capable of forming stable cell lines which retain the capacity to differentiate into cells from all three germ layers. This makes them of special significance in both regenerative medicine and toxicology. Induced pluripotent stem (iPS) cells may also provide a similar breadth of utility without some of the confounding ethical issues surrounding embryonic stem cells. An essential pre-requisite to the commercial and clinical application of stem cells are suitable cryopreservation protocols for long-term storage. Whilst effective methods for cryopreservation and storage have been developed for haematopoietic and mesenchymal stem cells, embryonic cells and iPS cells have proved more refractory. This paper reviews the current state of cryopreservation as it pertains to stem cells and in particular the embryonic and iPS cell.

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Piglets produced from in vivo blastocysts vitrified using the Cryologic Vitrification Method (solid surface vitrification) and a sealed storage container

Cited by 35 publications

References 18 publications

In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm

In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm

Recent advances toward the practical application of embryo transfer in pigs

Cryopreservation of Human Stem Cells for Clinical Application: A Review

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