Pigmented-anaerobic bacteria associated with canine periodontitis

Hardham, John M.; Dreier, Kimberly; Wong, Jason; Sfintescu, Cornelia; Evans, R. T.

doi:10.1016/j.vetmic.2004.12.018

Cited by 91 publications

(94 citation statements)

References 36 publications

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“…In veterinary dentistry, it is estimated that more than 80% of adult dogs have periodontal disease (18). Since these organisms have an important role in oral infection, their isolation and identification are required (12,18).…”

Section: Discussionmentioning

confidence: 99%

“…Since these organisms have an important role in oral infection, their isolation and identification are required (12,18). Periodontal bacteria such as E. corrodens, A. actinomycetemcomitans, P. gingivalis, P. intermedia and F. nucleatum are recognized as important opportunistic pathogens in the development of periodontal and non-oral diseases of humans and dogs (7,16,19).…”

Section: Discussionmentioning

confidence: 99%

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Detection of putative periodontal pathogens in subgingival specimens of dogs

Nishiyama¹,

Senhorinho²,

Gioso³

et al. 2007

Braz. J. Microbiol.

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In this study, the presence of putative periodontal organisms, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, Fusobacterium nucleatum, Dialister pneumosintes, Actinobacillus actinomycetemcomitans, Campylobacter rectus, Eikenella corrodens and Treponema denticola were examined from subgingival samples of 40 dogs of different breeds with (25) and without (15) periodontitis, by using the PCR method. The PCR products of each species showed specific amplicons. Of the 25 dogs with periodontitis, P. gingivalis was detected in 16 (64%) samples, C. rectus in 9 (36%), A. actinomycetemcomitans in 6 (24%), P. intermedia in 5 (20%), T. forsythensis in 5 (20%), F. nucleatum in 4 (16%) and E. corrodens in 3 (12%). T. denticola and D. pneumosintes were not detected in clinical samples from dogs with periodontitis. Moreover, P. gingivalis was detected only in one (6.66%) crossbred dog without periodontitis. Our results show that these microorganisms are present in periodontal microbiota of dogs with periodontitits, and it is important to evaluate the role of these putative periodontal microorganisms play in the periodontitis in household pets particularly, dogs in ecologic and therapeutic terms, since these animals might acquire these periodontopahogens from their respective owners.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Detection of putative periodontal pathogens in subgingival specimens of dogs

Nishiyama¹,

Senhorinho²,

Gioso³

et al. 2007

Braz. J. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Strain B106 T and Porphyromonas gulae B 243 (Hardham et al, 2003(Hardham et al, , 2005a were subjected to scanning electron microscopy to determine the cellular morphology. Briefly, 36-h liquid cultures in modified phytone/yeast/glucose medium (Hardham et al, 2003) were centrifuged at 1800 g. The medium was replaced with 3 % electron microscopygrade glutaraldehyde (Polysciences) in a 0.1 M phosphate buffer (pH 7.3) and incubated at 4 u C for 1 h. The cells were then washed twice in 0.1 M phosphate buffer (pH 7.3) and suspended in buffered 1 % osmium tetroxide (Polysciences) for 1 h at room temperature.…”

Section: Structural Analysismentioning

confidence: 99%

“…We identified numerous isolates whose 16S rRNA gene sequences did not have highly similar matches in the available databases, indicating that they may represent novel isolates. One group of these isolates (Table 1) appeared to represent a novel species originally referred to as 'Porphyromonas denticanis' (Hardham et al, 2003(Hardham et al, , 2005a. Herein, we propose that this novel species should be placed into a new genus with the name Odoribacter gen. nov.…”

mentioning

confidence: 90%