Pigmented Cutaneous Papillomatosis (Pigmented Epidermal Nevus) in Three Pug Dogs; Histopathology, Electron Microscopy and Analysis of Viral DNA by the Polymerase Chain Reaction

Narama, Isao; Kobayashi, Yoshiyasu; Yamagami, Takeshi; Ozaki, Kiyokazu; Ueda, Yachiyo

doi:10.1016/j.jcpa.2004.09.002

Cited by 24 publications

(34 citation statements)

References 11 publications

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“…Koilocytosis can be a subtle feature in pigmented plaques 5 and it was only identified within three biopsies in this study. Viral inclusions can be identified in pigmented plaques 12 , although this is not a consistent feature 11,15 , and it was not identified in any of the biopsies in this study. The clinical history, including breed of dog and location of the pigmented plaque, for all cases is listed in Table 1.…”

contrasting

confidence: 67%

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Detection of six novel papillomavirus sequences within canine pigmented plaques

et al. 2012

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In dogs, papillomaviruses are thought to cause oral and cutaneous papillomas and pigmented plaques. Eight canine papillomaviruses have been fully sequenced to date. Four of these canine papillomaviruses, including Canis familiaris papillomavirus (CPV)-3, CPV-4, CPV-5, and CPV-8, were amplified from pigmented plaques. Given this recent identification of several different canine papillomaviruses within pigmented plaques, it is likely that there are additional papillomavirus sequences that have not been previously identified. The aim of this study was to detect papillomavirus DNA sequences from pigmented plaques and identify potentially novel PV sequences through nucleotide sequence analysis. Polymerase chain reaction was used to amplify DNA sequences of the papillomavirus L1 gene from 27 pigmented plaques. Identification of novel papillomavirus sequences was based upon less than 90% shared DNA homology to any known papillomavirus. Ten different papillomaviruses were detected within the pigmented plaques, including 6 novel PV sequences. CPV-4 was detected within 41% (11/27) of the pigmented plaques, while CPV-5 was identified within 2 pigmented plaques and CPV-3 within a single pigmented plaque. A previously identified novel papillomavirus sequence was identified within 2 pigmented plaques in this study. The remaining 11 pigmented plaques contained 6 papillomavirus DNA sequences that have not been previously reported. These novel PV sequences were most similar to papillomaviruses that have been detected within canine pigmented plaques.

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confidence: 67%

“…Specific primers for CPV-4 were chosen since this was the most commonly detected CPV based upon initial sequencing results of the other 23/27 pigmented plaques. The specific primers for CPV-4 have been previously published and amplify a 194 base pair fragment 11 .…”

mentioning

confidence: 99%

Detection of six novel papillomavirus sequences within canine pigmented plaques

et al. 2012

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“…The antibody used in this study was originally prepared for detecting viral structural antigens (viral capsid L1 protein antigens) of bovine papillomavirus (BPV). Also, a rabbit polyclonal antibody for BPV is known to cross-react with canine cutaneous papillomavirus [1,9,12]. In this study, we were able to detect viral antigens with BPV antibody in tissues affected by canine oral papilloma.…”

Section: Discussionmentioning

confidence: 67%

“…Three different primers used in this study were selected from the primers information by others [4,9,14]. Primer sets 1 and 2 were specific for the diagnosis COPV [14] or canine cutaneous papillomavirus [9], respectively. Primers set 3 consisted of degenerate primers able to amplify a broad range of human papillomavirus L1 genes [4].…”

Section: Methodsmentioning

confidence: 99%

“…DNAzol Reagent (Invitrogen, USA) was used to extract DNA according of the manufacturer's instructions. The PCR reaction was carried out with the Maxime PCR premix kit (Intron, Korea), and each amplification followed the conditions as previously described [4,9,14]. The PCR products were analyzed by electrophoresis in a 2.0% agarose gel stained with ethidium bromide and observed under UV transillumination.…”

Section: Methodsmentioning

confidence: 99%

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Characterization of canine oral papillomavirus by histopathological and genetic analysis in Korea

Yhee

Kwon²,

Kim

et al. 2010

J Vet Sci

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In August 2008, forty dogs out of 400 developed oral warts in a breeding farm in Korea. Canine oral papilloma infection is a common disease in dogs. However, there has been no report of an outbreak of canine oral papillomavirus (COPV) in a group of dogs or in dog breeding farms in Korea, and the genetic analysis of COPV in Korea has yet to be performed. This study diagnosed canine oral papilloma from the oral samples of these dogs based on histopathological examination and immunohistochemistry. Polymerase chain reaction was applied to amplify the corresponding products using pre-existing primer sets for COPV and a universal human papillomavirus targeting L1 gene. Further genetic analysis of the major viral capsid gene L1 confirms the sequences of Korean COPV, which shows a close relationship to previously reported COPV. This study describes the histopathological and immunohistochemical characteristics of canine oral papilloma in a group of breeding dogs in Korea and discloses the complete L1 gene sequences of Korean COPV.

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2018

Breed Predispositions to Disease in Dogs and Cats

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Pigmented Cutaneous Papillomatosis (Pigmented Epidermal Nevus) in Three Pug Dogs; Histopathology, Electron Microscopy and Analysis of Viral DNA by the Polymerase Chain Reaction

Cited by 24 publications

References 11 publications

Detection of six novel papillomavirus sequences within canine pigmented plaques

Detection of six novel papillomavirus sequences within canine pigmented plaques

Characterization of canine oral papillomavirus by histopathological and genetic analysis in Korea

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