Pilocarpine-induced increases in the activity of 6-phosphofructo-2-kinase and the fructose-2,6-bisphosphate content of rat salivary glands

Nicolau, José Carlos; Souza, Douglas Nesadal de; Martins, Hilana Rickli Fiuza

doi:10.1016/0003-9969(92)90104-g

Cited by 11 publications

(7 citation statements)

References 22 publications

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“…16 Thus, the variations in glandular glycogen metabolism are used in vitro as an indicator of their functional activity. 17 In the present work, the basal concentration of glycogen were different in the rats treated with these cytostatic agents. There was a higher concentration of glycogen in the glands of the animals that were administered 5-FU+LV, compared to the group treated with cyclophosphamide.…”

Section: Rats Treated With Cyclophosphamidementioning

confidence: 41%

5-Fluorouracil and Cyclophosphamide modify functional activity in submandibular gland of rats.

et al. 2020

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Objective: Chemotherapy treatment against cancer produce systemic toxicities, among which are those related to important structures of the stomatognathic system and its functional activity. 5 Fluorouracil (5-FU) and cyclophosphamide (Cf) are drugs widely used in solid tumors and in bone marrow transplantation, respectively. The objective of this work was to evaluate the toxicity of these drugs regarding functional activity of the submandibular glands, by measuring the percentage of glycogen consumption in two experimental models. Material and Methods: 84 male Wistar rats aged three months were used, housed in individual cages, with controlled temperature and lighting and ad libitum diet. They were divided into four experimental groups: 1) Control (C); 2) Treated with 5-FU+leucovorin (LV) at 20 and 10mg/Kg of body weight respectively for five consecutive days; 3) treated with Cf i.p. at 50mg/Kg of body weight for two consecutive days; and 4) rats with paired feeding (PF): for five and two days respectively, the amount administered resulted from the average of the ingested food of groups 2 and 3. Both submandibular glands were excised. The submandibular glycogen concentration was analyzed at initial time (t0) and after 60 minutes of mechanical stimulation (t60). Results: the average variation changed significantly between time 0 and 60 in the groups C and PF. (p-value=0.0001), the 5-FU + LV treatment group had an average concentration higher at t0 than groups C and PF, without significant consumption at T60. While group Cf showed a lower average concentration at time 0 with respect to groups C and PF, without significant consumption at T60. Conclusion: 5-FU+LV and Cf affect the metabolism of carbohydrates, decreasing the use of glycogen as a metabolic substrate. In the present experimental model, the toxicity of these drugs affected the functional activity of the submandibular gland.

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Section: Rats Treated With Cyclophosphamidementioning

confidence: 41%

5-Fluorouracil and Cyclophosphamide modify functional activity in submandibular gland of rats.

et al. 2020

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“…En condiciones fisiológicas los procesos de secreción salival requieren del aporte de hidratos de carbono como sustrato metabólico. Varios investigadores han establecido la dependencia de las glándulas submandibulares de los mecanismos de glucogenólisis y glucólisis como principales fuentes metabólicas para llevar a cabo la secreción salival 7 . De este modo, las variaciones del metabolismo del glucógeno glandular son utilizadas in vitro como indicador de la actividad funcional de las mismas 8 .…”

Section: Discussionunclassified

Efecto de ciclofosfamida sobre el metabolismo de los hidratos de carbono en glándula submandibular de ratas

Mazzeo¹,

Bachmeier²,

López³

et al. 2018

RevFacOdont

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Efecto de ciclofosfamida sobre el metabolismo de los hidratos de carbono en glándula submandibular de ratasEffect of cyclophosphamide on the metabolism of carbohydrates in the submandibular gland of rats AbstractObjective: During the period of chemotherapy, the appearance of serious systemic toxicities is one of the main clinical problems, among which are those related to the stomatognathic system. Cyclophosphamide is a drug widely used in solid tumors and bone marrow transplantation. The objective of this work was to evaluate the toxicity of cyclophosphamide on the functional activity of the submandibular glands of Wistar rats in an experimental model. Methods: We used 42 male Wistar rats aged 3 months, housed in individual cages, with temperature and controlled lighting and free diet. They were divided into three experimental groups: 1) Control (C), 2) treated with cyclophosphamide (Cf,) applying an i.p. of 50 mg / Kg of body weight during two consecutive days and 3) rats with paired feeding (AA): during two days the amount administered resulted from the average of the ingested food of group 2 .Both submandibular glands were removed. The submandibular glycogen concentration was analyzed in initial time (t0) and after 60 minutes of mechanical stimulation (t60). Results: The average variations changed significantly between time 0 and 60 in groups C and AA (p-value = 0.0001), Cf had a lower average concentration in time 0 with respect to C and AA, without significant consumption in time 60. Conclusion: Cf would affect the metabolism of carbohydrates, decreasing the use of glycogen as a metabolic substrate. In the present experimental model the toxicity of this drug reduces the functional activity of the submandibular gland.

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“…Evidence of a relationship between the mobilization of glycogen and the process of secretion in salivary glands has been provided by experiments using adrenergic and muscariniccholinergic agonists [8][9][10][11].…”

Section: Introductionmentioning

confidence: 98%

Lithium Induces Glycogen Accumulation in Salivary Glands of the Rat

Souza

Mendes

Nogueira

et al. 2015

Biol Trace Elem Res

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Lithium is administered for the treatment of mood and bipolar disorder. The aim of this study was to verify whether treatment with different concentrations of lithium may affect the glycogen metabolism in the salivary glands of the rats when compared with the liver. Mobilization of glycogen in salivary glands is important for the process of secretion. Two sets of experiments were carried out, that is, in the first, the rats received drinking water supplemented with LiCl (38,25 and 12 mM of LiCl for 15 days) and the second experiment was carried out by intraperitoneal injection of LiCl solution (12 mg/kg and 45 mg LiCl/kg body weight) for 3 days. The active form of glycogen phosphorylase was not affected by treatment with LiCl considering the two experiments. The active form of glycogen synthase presented higher activity in the submandibular glands of rats treated with 25 and 38 mM LiCl and in the liver, with 25 mM LiCl. Glycogen level was higher than that of control in the submandibular glands of rats receiving 38 and 12 mM LiCl, in the parotid of rats receiving 25 and 38 mM, and in the liver of rats receiving 12 mM LiCl. The absolute value of glycogen for the submandibular treated with 25 mM LiCl, and the liver treated with 38 mM LiCl, was higher than the control value, although not statistically significant for these tissues. No statistically significant difference was found in the submandibular and parotid salivary glands for protein concentration when comparing experimental and control groups. We concluded that LiCl administered to rats influences the metabolism of glycogen in salivary glands.

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Pilocarpine-induced increases in the activity of 6-phosphofructo-2-kinase and the fructose-2,6-bisphosphate content of rat salivary glands

Cited by 11 publications

References 22 publications

5-Fluorouracil and Cyclophosphamide modify functional activity in submandibular gland of rats.

5-Fluorouracil and Cyclophosphamide modify functional activity in submandibular gland of rats.

Efecto de ciclofosfamida sobre el metabolismo de los hidratos de carbono en glándula submandibular de ratas

Lithium Induces Glycogen Accumulation in Salivary Glands of the Rat

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