Pilot‐Plant Proof‐of‐Concept for Integrated, Countercurrent, Two‐Stage, Enzyme‐Assisted Aqueous Extraction of Soybeans

Moura, J. M. L. N. de; Maurer, Devin L.; Jung, Stéphanie; Johnson, Lawrence A.

doi:10.1007/s11746-011-1831-y

Cited by 43 publications

(34 citation statements)

References 16 publications

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“…Soybean cotyledon fiber (6.4% protein dwb) was produced in the pilot plant of CCUR, Iowa State University, via two-stage counter-current EAEP. 5 To obtain DDGS or soy-enhanced DDGS, the first batch of corn ethanol fermentation was performed in the Iowa State University BioCentury Research Farm according to the conventional method 21 with 100% water. A second batch of corn fermentation was performed under similar conditions except that 50% of the water was replaced with soy skim fraction that was obtained from EAEP.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Solid-State Fermentation of Soybean and Corn Processing Coproducts for Potential Feed Improvement

Lio

Wang

2012

J. Agric. Food Chem.

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Two agro-industrial coproducts, soybean cotyledon fiber and distiller's dried grains with solubles (DDGS), were used as substrates to evaluate the effect of coculturing three different fungi, Aspergillus oryzae, Trichoderma reesei, and Phanerochaete chrysosporium, on enzyme production by solid-state fermentation (SSF). When soybean fiber was used as the substrate, a maximum xylanase activity of 757.4 IU/g and a cellulase activity of 3.2 IU/g were achieved with the inoculation and incubation of T. reesei and P. chrysosporium for 36 h, followed by A. oryzae for an additional 108 h. This inoculation scheme also resulted in the highest xylanase activity of 399.2 IU/g compared to other fungi combinations in the SSF of DDGS. A large-scale SSF by this fungus combination produced fermented products that had xylanase and cellulase activities of 35.9−57.0 and 0.4−1.2 IU/g, respectively. These products also had 3.5−15.1% lower fiber and 1.3−4.2% higher protein contents, suggesting a potential feed quality improvement.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

“…EAEP produces soybean cotyledon fiber and soy skim as coproducts. 5 The low-valued soybean cotyledon fiber has high fiber content, and this limits its use for nonruminant feed. Thus, SSF may have a great potential in producing enzymes to break down its fiber to improve its digestibility.…”

Section: ■ Introductionmentioning

confidence: 99%

Solid-State Fermentation of Soybean and Corn Processing Coproducts for Potential Feed Improvement

Lio

Wang

2012

J. Agric. Food Chem.

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“…The washing step also resulted in increased usage of water. Mechanical treatment of soybeans (cracking using a corrugated roller mill, flaking using smooth roller mill to 0.25 mm thickness, and extruding at 100 • C) before EAEP ruptured the soybean cell walls, composed of cellulose, hemicellulose, pectin, and lignin [2]. The addition of PCX enzymes in SSF enabled further breakdown of pectin and hemicellulose to release cellulose, xylose, and arabinose.…”

Section: Treatment Of Insoluble Fibermentioning

confidence: 99%

“…Enzyme-assisted aqueous extraction process (EAEP) is an environmentally friendly alternative to chemical (organic solvent such as hexane) or mechanical extraction of oil from soybeans [1][2][3]. In comparison to conventional oil extraction methods, EAEP provides sustainable, safe, and low-cost extraction of oil from soybeans, with simultaneous production of valuable co-products.…”

Section: Introductionmentioning

confidence: 99%

Ethanol Production by Soy Fiber Treatment and Simultaneous Saccharification and Co-Fermentation in an Integrated Corn-Soy Biorefinery

et al. 2018

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Insoluble fiber (IF) recovered from the enzyme-assisted aqueous extraction process (EAEP) of soybeans is a fraction rich in carbohydrates and proteins. It can be used to enhance ethanol production in an integrated corn-soy biorefinery, which combines EAEP with traditional corn-based ethanol processing. The present study evaluated IF as a substrate for ethanol production. The effects of treatment of IF (soaking in aqueous ammonia (SAA), liquid hot water (LHW), and enzymatic hydrolysis), primarily simultaneous saccharification and co-fermentation (SSCF), as well as scaling up (250 mL to 60 L) on ethanol production from IF alone or a corn and IF slurry were investigated. Enzymatic hydrolysis (pectinase, cellulase, and xylanase, each added at 5% soy solids during simultaneous saccharification and fermentation/SSCF) was the best treatment to maximize ethanol production from IF. Ethanol yield almost doubled when SSCF of IF was performed with Saccharomyces cerevisiae and Escherichia coli KO11. Addition of IF in dry-grind corn fermentation increased the ethanol production rate (~31%), but low ethanol tolerance of E. coli KO11 was a limiting factor for employing SSCF in combination corn and IF fermentation. Nonlinear Monod modeling accurately predicted the effect of ethanol concentration on E. coli KO11 growth kinetics by Hanes-Woolf linearization. Collectively, the results from this study suggest a potential of IF as a substrate, alone or in dry-grind corn fermentation, where it enhances the ethanol production rate. IF can be incorporated in the current bioethanol industry with no added capital investment, except enzymes.

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“…[45] extracted total phenolic contents from five citrus peels (Yen Ben lemon, Meyer lemon, grapefruit, mandarin and orange) by EAAE using different enzymes and the recovery was highest with celluzyme MX enzyme. Considerable improvements have been recently achieved in enzyme assisted aqueous extraction, processing of extruded soybean flakes [46,47] and soybean flour [48] but still not satisfactory achievement was getting. While the most advanced EACP for extracting extruded soybean flakes with the aid of a protease enzyme in a countercurrent two-stage strategy resulted in higher oil extraction yield of 99% as an extrusion process broken the cell wall which enhances the reaction rate of protease, an unstable emulsion obtained during the process was easily demulsified by adjusting the pH of the emulsion to the isoelectric point of the soy proteins (4.5) [19,47,49].…”

Section: Pulsed-electric Field Extraction (Pef)mentioning

confidence: 99%

Novel Eco-Friendly Techniques for Extraction of Food Based Lipophilic Compounds from Biological Materials

Ae¹,

Singh²,

Nc³

et al. 2016

Nat Prod Chem Res

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Plant-based lipophilic compounds such as edible oils, phytochemicals, flavors, fragrances and colours are valuable products in the food, pharmaceutical and chemical industry. Extraction is one of the key processing steps in recovering and purifying lipophilic ingredients contained in plant-based materials. Lipophilic compounds are found in the cells of oil bearing plant seeds. Solvent extraction is the most common commercially used extraction method for low oil content materials. Solvent extraction has more extractability than existing commercial methods, but it also passes serious limitations and environmental issues. The Environmental Protection Agency has been much concerned regarding safety and environmental emissions associated with hexane usage in the solvent extraction process. Bio-solvents resulting from renewable raw materials and in particular from the biomass may become a solution of the existing problem. But the existing conventional extraction methods cannot respond to such solvent and hence need for new novel "Green" extraction technology having potential to overcome all limitations of existing technologies. This review will discuss the various possible novel eco-friendly extraction methods, their scientific concepts, principles, challenges, limitations and technological effort needed for successful implementation at the production scale.

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Pilot‐Plant Proof‐of‐Concept for Integrated, Countercurrent, Two‐Stage, Enzyme‐Assisted Aqueous Extraction of Soybeans

Cited by 43 publications

References 16 publications

Solid-State Fermentation of Soybean and Corn Processing Coproducts for Potential Feed Improvement

Solid-State Fermentation of Soybean and Corn Processing Coproducts for Potential Feed Improvement

Ethanol Production by Soy Fiber Treatment and Simultaneous Saccharification and Co-Fermentation in an Integrated Corn-Soy Biorefinery

Novel Eco-Friendly Techniques for Extraction of Food Based Lipophilic Compounds from Biological Materials

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