Pilot study: Autologous platelet‐rich plasma used in a topical cream for facial rejuvenation

Abstract

The basic human need to look young has led to many concepts in facial rejuvenation. One such concept is the adaptation of platelet-rich plasma (PRP) for dermatologic uses. 1 PRP was originally developed to improve healing in orthopedic and dental surgery, but is now used in plastic surgery via injection for wound healing and hair growth. 2,3 The next horizon is the adaptation of PRP for topical uses in cosmetic formulations.Platelets are composed of a cytoskeleton and intracellular structures such as glycogen, lysosomes, and two granules, the dense granule and the alpha-granule. The granules are the most valuable structures for the development of the current topical product. The dense granule has adenosine diphosphate (ADP), adenosine triphosphate (ATP), serotonin, and calcium, while the alpha-granule contains clotting factors, growth factors, and proteins. 4 The main goal for the topical product is to keep the enclosed cellular components active throughout the intended time period of use and obtain enough platelets to be considered platelet-rich plasma.The normal platelet count in human blood is 150 000-350 000/ microliter. PRP is defined as 1 000 000 platelets per microliter in a small volume of plasma with a full complement of clotting factors. There are seven key growth factors in PRP: platelet-derived growth factors (PDGFaa, PDGFbb, PDGFab), transforming growth factors Abstract Background: Platelet rich plasma (PRP) is traditionally used as an injectable material for enhanced healing, hair growth, and facial rejuvenation. Aims: This research examined the novel use of topical autologously sourced PRP added to a preservative cosmetic base and applied twice daily to the face following electroporation for 8 weeks. Methods: 20 healthy female and male subjects 30-60 years of age were enrolled in this single-site, investigator blinded, vehicle controlled split-face study to evaluate the effect of a PRP-containing serum versus the serum alone on facial photoaging. Results: 90 day stability for the PRP in a preservative serum was achieved with refrigeration at 4 degrees Celsius. Facial skin biopsy histologic findings included improved rete peg architecture. Immunohistochemical analysis showed upregulation for collagen type I with qPCR data demonstrating concomitant upregulation of mRNA for collagen after 8 weeks of topical PRP use. Conclusion: These pilot study findings may indicate value for topical PRP in facial rejuvenation. K E Y W O R D S aging skin, cosmetics, facial rejuvenation, platelet-rich plasma, PRPThis is an open access article under the terms of the Creat ive Commo ns Attri butio n-NonCo mmercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

Efficacy and Safety of Autologous Platelet Concentrate in the Treatment of Photoaging on the Back of the Hands

“…These factors not only aid in clotting but also drive angiogenesis and promote tissue repair and regeneration [10]. Multiple authors have shown that topical application or intradermal injection of PRP and its growth factors produce favorable cutaneous changes: clinical (restores its vitality, increases its thickness, recovers its elastic consistency, improves blood circulation, increases its smoothness, decreases its wrinkles and improves their appearance) [2,10,11] histopathological (increases the number of fibroblasts, collagen fibers and blood vessel basement membranes) [2,10,12] immunohistochemical (improves collagen expression type I, III and IV) [13][14][15] and the genetic material (through the polymerase chain reaction expression of three target genes, such as: collagen IA, matrix metalloproteinase gene 1 and protein rich in proline of keratinocytes) [13,14]. PRP has been used as an adjunct to multiple skin rejuvenation treatments (lipotransference, laser, peeling, lifting).…”

Does platelet-rich plasma promote facial rejuvenation? Revising the latest evidence in a narrative review

“…Initial search retrieved 539 articles and from these, 523 were excluded based on title, abstract and exclusion criteria. A total of 16 articles were included in the review,[ 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 ] with an aggregate of 586 participants [ Table 1 ]. Among the 16 articles, in 10 studies (62.5%), PRP was injected in monotherapy, in five studies (31.2%), PRP was topically applied following ablative laser treatment (CO 2 or erbium), microneedling or electroporation and in one study (6.3%) PRP was injected in conjunction with autologous fat.…”

Microneedling dengan Platelet-rich Plasma (PRP) untuk Skar Akne Atrofi

“…Dengan konsentrasi platelet di atas rata-rata dan penuh komplemen faktor pembekuan, PRP dapat berkontribusi meningkatkan penyembuhan (jumlah platelet normal 150.000-350.000/μL). 7,18,19 PRP digunakan di berbagai cabang ilmu kedokteran seperti estetika, bedah plastik, ortopedi, kedokteran gigi, trauma dan penyembuhan luka, oftalmologi, serta gastroenterologi sebagai bagian dari kedokteran regeneratif. Kedokteran regeneratif merupakan bidang penelitian dan aplikasi klinis secara interdisipliner yang berfokus pada perbaikan, penggantian, atau regenerasi sel, jaringan, atau organ untuk mengembalikan fungsi yang rusak.…”

“…Sentrifugasi pertama (hard spin) memisahkan Selanjutnya ditambahkan trombin atau kalsium klorida sebagai aktivator platelet. 18,21 Trombin dan kalsium klorida merupakan penginduksi agregasi yang berfungsi mengaktifkan platelet dan menstimulasi degranulasi, mengakibatkan pelepasan faktor pertumbuhan. Studi terkini menemukan bahwa penggunaan agregator tersebut tidak diperlukan karena saat pemberian, platelet secara otomatis dilepaskan dan siap untuk berfungsi.…”