Pimarane-type Diterpenes: Antimicrobial Activity against Oral Pathogens

Porto, Thiago S.; Rangel, Rander; Furtado, Niege Araçari Jacometti Cardoso; Carvalho, Tiago; Martins, Carlos Henrique Gomes; Veneziani, Rodrigo Cássio Sola; Costa, Fernando B. Da; Vinholis, Adriana Helena Chicharo; Cunha, Wilson Roberto; Heleno, Vladimir Constantino Gomes; Ambrósio, Sérgio Ricardo

doi:10.3390/molecules14010191

Cited by 89 publications

(95 citation statements)

References 27 publications

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“…However, various adverse effects such as tooth and restoration staining, increased calculus formation, diarrhea, and disarrangements of the oral and intestinal flora have been associated with the use of these chemicals (Chung et al 2006, More et al 2008. These drawbacks justify the search for new effective anticariogenic compounds that could aid caries prevention (Porto et al 2009). …”

Section: Resultsmentioning

confidence: 99%

Antimicrobial activity of apitoxin, melittin and phospholipase A2 of honey bee (Apis mellifera) venom against oral pathogens

Leandro

Mendes

Casemiro

et al. 2015

An. Acad. Bras. Ciênc.

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In this work, we used the Minimum Inhibitory Concentration (MIC) technique to evaluate the antibacterial potential of the apitoxin produced by Apis mellifera bees against the causative agents of tooth decay. Apitoxin was assayed in natura and in the commercially available form. The antibacterial actions of the main components of this apitoxin, phospholipase A 2 , and melittin were also assessed, alone and in combination. The following bacteria were tested: Streptococcus salivarius, S. sobrinus, S. mutans, S. mitis, S. sanguinis, Lactobacillus casei, and Enterococcus faecalis. The MIC results obtained for the commercially available apitoxin and for the apitoxin in natura were close and lay between 20 and 40µg / mL, which indicated good antibacterial activity. Melittin was the most active component in apitoxin; it displayed very promising MIC values, from 4 to 40µg / mL. Phospholipase A 2 presented MIC values higher than 400µg / mL. Association of mellitin with phospholipase A 2 yielded MIC values ranging between 6 and 80µg / mL. Considering that tooth decay affects people's health, apitoxin and its component melittin have potential application against oral pathogens.

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Section: Resultsmentioning

confidence: 99%

Antimicrobial activity of apitoxin, melittin and phospholipase A2 of honey bee (Apis mellifera) venom against oral pathogens

Leandro

Mendes

Casemiro

et al. 2015

An. Acad. Bras. Ciênc.

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“…The MICs of the samples against the test bacterial strains were performed according to the broth microdilution method. The entire procedure was performed in a laminar flow hood, with all of the glassware, pipette tips, culture media and 96-well microplates sterilised (Porto et al, 2009;Carvalho et al, 2011). Samples were dissolved in 1 mg ml -1 dimethyl sulphoxide (DMSO), followed by dilution in tryptic soy broth (Difco) for aerobic and Schaedler broth (Difco) supplemented with haemin (5 μg ml −1 ) and vitamin K1 (10 μg ml −1 ) for anaerobic; concentrations ranged from 400 to 10 µg ml -1 .…”

Section: Minimum Inhibitory Concentration (Mic): Broth Microdilution mentioning

confidence: 99%

Antioxidant and antimicrobial activity of Kielmeyera coriacea Mart. and Zucc

Aquin¹,

Moura²,

Morais³

et al. 2013

J. Med. Plants Res.

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Kielmeyera coriaceae Mart. and Zucc. belongs to Clusiaceae family and is largely distributed in the cerrado Brazilian biome. In this study, phytochemical screening, the antioxidant and antimicrobial activities of aerial parts of the K. coriacea were evaluated. The extracts showed the presence of terpenoids, saponins, triterpenes, steroids, flavonoids, tannins and phenolic compounds. Ethanolic extract and partitions of plant parts of K. coriacea showed a very strong antioxidant activity, in particular, the inner bark of the plant. The ethanolic extract and the cyclohexane fraction of the inner bark showed high antimicrobial activity against oral pathogens with minimum inhibitory concentrations (MIC) between 3.1 and 100 μg ml −1 . The cyclohexane fraction was the most active with CIMs of 6.2 μg ml −1 for most of the tested bacteria, including Streptococcus mutans, the main etiological agent of dental caries. The ethanolic extract exhibited the highest antibacterial activity against Streptococcus mitis, with MIC value of 3.1 μg ml −1 , a value lower than that found by the positive control. The antioxidant and antimicrobial activities could be due to the different phytochemical classes presented in the ethanolic extract and partitions studied. The antioxidant activity and the antimicrobial activity showed by the ethanolic extract and cyclohexene fraction of the inner bark were considered promising.

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“…Diterpenes also display significant antimicrobial activity (Tatsimo et al, 2005;Kuzma et al, 2007;Ndi et al, 2007;Fukumoto et al, 2008;Porto et al, 2009a;Porto et al, 2009b;Carvalho et al, 2011;Andrade et al, 2011;Moreira et al, 2016;Moreti et al, 2017;Veneziani et al, 2017). Ambrosio et al (2004) isolated and identified diterpenes of the pimarane type from V. arenaria baker root extracts.…”

Section: Introductionmentioning

confidence: 99%

Diterpenes of the pimarane type isolated from Viguiera arenaria: Promising in vitro biological potential as therapeutic agents for endodontics

Marangoni¹,

Moraes²,

Utrera³

et al. 2018

J. Pharmacognosy Phytother.

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Viguiera arenaria, family Asteraceae, is a plant that contains diterpenoids, which make this species potentially applicable in endodontics. More specifically, V. arenaria contains diterpenes of the pimarane type, which display various classic biological activities. This study evaluates the antibiofilm activity, the time-kill curve, and the inhibitory concentration index of diterpenes of the pimarane type (entpimara-8(14),15-dien-19-oic acid, ent-8(14),15-pimaradien-3β-ol, and ent-8(14),15-pimaradien-3β-19-oic acid sodium salt, designated diterpenes I, II, and III, respectively) toward nine anaerobic bacteria commonly found in endodontic infections; this study also assesses the cytotoxic activity of these diterpenes against human fibroblasts. According to the antibiofilm assay, diterpenes I, II, and III inhibit at least 50% of all the bacteria. On the basis of the time-kill curve experiments, the behavior of these diterpenes depends on the tested bacteria, diterpene concentration, and microorganism sensitivity. Synergism of diterpenes I and II with chlorhexidine (CDH) was higher against P. gingivalis (clinical isolate) and Aggregatibacter actinomycetemcomitans (ATCC). As for diterpene III, synergism with CDH is higher against P. micros. As revealed by the XTT assay, none of the diterpenes of the pimarane type tested here are cytotoxic. Hence, diterpenes I, II, and III are promising biomolecules and may provide therapeutic solutions in the field of endodontics.

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Pimarane-type Diterpenes: Antimicrobial Activity against Oral Pathogens

Cited by 89 publications

References 27 publications

Antimicrobial activity of apitoxin, melittin and phospholipase A2 of honey bee (Apis mellifera) venom against oral pathogens

Antimicrobial activity of apitoxin, melittin and phospholipase A2 of honey bee (Apis mellifera) venom against oral pathogens

Antioxidant and antimicrobial activity of Kielmeyera coriacea Mart. and Zucc

Diterpenes of the pimarane type isolated from Viguiera arenaria: Promising in vitro biological potential as therapeutic agents for endodontics

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