Pinched Inlet Split Flow Thin Fractionation for Continuous Particle Fractionation:  Application to Marine Sediments for Size-Dependent Analysis of PCDD/Fs and Metals

Moon, Myeong Hee; Kim, Hyun Joo; Kwon, So Yeon; Lee, Se Jin; Chang, Yoon Seok; Lim, Hyun

doi:10.1021/ac049968u

Cited by 31 publications

(19 citation statements)

References 26 publications

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“…PFF has previously been demonstrated in a microchannel device as a way to separate microparticles by size with continuous flow . Targeted applications include size determination of air‐borne particles, marine‐sediment measurement, and whole‐blood separation . Channel geometry, particle size, fluid transport, and volumetric flow ratio of input‐channel fluids are all known to affect the trajectories of particle motion through the device.…”

Section: Introductionmentioning

confidence: 99%

Hydrodynamic separation of particles using pinched‐flow fractionation

2013

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Rigid particles transported through a pinched-flow fractionation (PFF) device are simulated using boundary-integral methods (BIM). The PFF device separates particles by size using a bifurcated microfluidic channel. The critical flow ratio of the two input channels required to achieve complete separation of large and small particles decreases with increasing diameter of the larger particles relative to the pinch height, and is nearly independent of the smaller particle size. A narrow pinch with a square exit was shown to have the lowest critical flow ratio and was selected as the model device to be fabricated. Experiments conducted using this device confirm that the larger particles exit further from the top wall than do the smaller particles, due to steric exclusion, and the final exit positions are within a few percent of the simulation results. It is shown that BIM is a valuable tool in the design of microfluidic devices.

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Section: Introductionmentioning

confidence: 99%

Hydrodynamic separation of particles using pinched‐flow fractionation

2013

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“…Numerous techniques have been developed to achieve this purpose and in general can be divided into two categories, active and passive. In the case of active separation methods, which include electrophoresis, 1,2 dielectrophoresis, [3][4][5][6] magnetophoresis, [7][8][9] ultrasound, 10,11 centrifugation, 12 gravitation, 13,14 and optical manipulation, [15][16][17] an external energy source is normally required to manipulate the migration of particles in the microfluidic environment. Although these active methods provide good separation accuracy, they are normally limited to low throughput and require a precision instrument for the additional energy source.…”

Section: Introductionmentioning

confidence: 99%

Multistage-multiorifice flow fractionation (MS-MOFF): continuous size-based separation of microspheres using multiple series of contraction/expansion microchannels

et al. 2011

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Previously we introduced a novel hydrodynamic method using a multi-orifice microchannel for size-based particle separation, which is called a multi-orifice flow fractionation (MOFF). The MOFF has several advantages such as continuous, non-intrusive, and minimal power consumption. However, it has a limitation that the recovery yield is relatively low. Although the recovery may be increased by adjusting parameters such as the Reynolds number and central collecting region, poor purity inevitably followed. We newly designed and fabricated a microfluidic channel for multi-stage multi-orifice flow fractionation (MS-MOFF), which is made by combining three multi-orifice segments, and consists of 3 inlets, 3 filters, 3 multi-orifice segments and 5 outlets. The structure and dimensions of the MS-MOFF were determined by the hydrodynamic principles to have constant Reynolds numbers at each multi-orifice segment. Polystyrene microspheres of two different sizes (7 μm and 15 μm) were tested. With this device, we made an attempt to improve recovery and minimize loss of purity by collecting and re-separating non-selected particles of the first separation. The final recovery successfully increased from 73.2% to 88.7% while the final purity slightly decreased from 91.4% to 89.1% (for 15 μm). These values were never achievable with the single-stage MOFF (SS-MOFF) having only one multi-orifice segment in our previous work. The MS-MOFF channel will be useful for clinical applications, such as separation of circulating tumor cells (CTC) or rare cells from human blood samples.

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“…The peaks were quantified when the criteria were the following: (1) the isotope ratios were within ±15% of theoretical values and (2) the signal noise ratio ≥2.5. Recoveries of the 13 C-labelled PCDD/Fs internal standards in the environmental samples ranged from 50 to 120%, a satisfactory range according to the EPA method 1613 [6,14,22,23]. Leaching tests for heavy metals were performed using the methods of the Korea Standard Leaching Test which is based on the US EPA method 1311 [14,24].…”

Section: Methodsmentioning

confidence: 99%