Pinewood Nematode Alters the Endophytic and Rhizospheric Microbial Communities of Pinus massoniana

Zhang, Wei; Wang, Xuan; Li, Yongxia; Liu, Zhenkai; Li, Dongzhen; Wen, Xiaojian; Feng, Yuqian; Zhang, Xingyao

doi:10.1007/s00248-020-01619-1

Cited by 22 publications

(24 citation statements)

References 51 publications

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“…However, opposite results were obtained from Ma et al (2020) demonstrated that there were no signi cant differences of rhizospheric bacterial diversities between healthy pines and wilted pines. Besides, another investigation showed that B. xylophilus infection appeared to reduce soil bacterial diversity (Shi et al, 2015), similar ndings were reported by Zhang et al (2020) demonstrated that B.…”

Section: Discussionsupporting

confidence: 78%

Variations of Phyllosphere and Rhizosphere Microbial Communities of Pinus koraiensis Infected by Bursaphelenchus xylophilus

Deng

Zhou

et al. 2021

Microb Ecol

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Pinewood nematode, Bursaphelenchus xylophilus, as one of the greatest threats to pine trees, is spreading all over the world. During the nematode's pathogenesis, plant microorganisms play important roles.The phyllosphere and rhizosphere bacterial and fungal communities associated with healthy P. koraiensis (PKa) and P. koraiensis infected by B. xylophilus at the early (PKb) and last (PKc) stages were analyzed. Pine wood nematode (PWD) could increase the phyllosphere and rhizosphere microbial community diversity, and slight shifts of the microbial diversity were observed at the early stage of infection. Generally speaking, an increase in microbial diversity with more serious symptomatic phase was obvious and visible. The phyllosphere and rhizosphere microbial community potentially changed caused by B. xylophilus infection of P. koraiensis. With the infection of B. xylophilus in P. koraiensis, Bradyrhizobium (rhizosphere bacteria), Massilia (phyllosphere bacteria), Phaeosphaeriaceae (rhizosphere fungi) were the major contributors to the differences in community compositions among different treatments. With the infection of PWD, most of the bacterial groups tended to be co-excluding rather than co-occurring. These changes would correlate with microbial ability to suppress plant pathogen, enhancing the understanding of disease development and providing guidelines to pave the way for its possible management.

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Section: Discussionsupporting

confidence: 78%

Variations of Phyllosphere and Rhizosphere Microbial Communities of Pinus koraiensis Infected by Bursaphelenchus xylophilus

Deng

Zhou

et al. 2021

Microb Ecol

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“…A recent study showed that PWN infection can significantly alter the endophytic and rhizospheric microbial community structures of P . massoniana [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, a decline in host health can change root exudates, which can directly affect microbial communities in rhizosphere soil rather than bulk soil. A recent study showed that PWN infection can significantly alter the endophytic and rhizospheric microbial community structures of P. massoniana [54].…”

Section: Discussionmentioning

confidence: 99%

“…tabulaeformis, and alter the rhizosphere and endophytic microbial community of P . massoniana [ 53 , 54 ]. A recent study showed that PWD only affects bacterial community structure and functional structure in roots and needles, respectively, and did not affect host bacterial diversity [ 55 ].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies showed that the fungal species that were dominant in P. thunbergii had a compatible relationship with PWN to increase nematode reproduction, while some had an incompatible or neutral relationship [51,52]. PWN infection can alter root-associated fungal communities in P. tabulaeformis, and alter the rhizosphere and en-dophytic microbial community of P. massoniana [53,54]. A recent study showed that PWD only affects bacterial community structure and functional structure in roots and needles, respectively, and did not affect host bacterial diversity [55].…”

Section: Introductionmentioning

confidence: 99%

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The Impact of Pine Wood Nematode Infection on the Host Fungal Community

Liu

et al. 2021

Microorganisms

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Pine wilt disease (PWD), caused by pinewood nematode (PWN) Bursaphelenchus xylophilus, is globally one of the most destructive diseases of pine forests, especially in China. However, little is known about the effect of PWD on the host microbiome. In this study, the fungal community and functional structures in the needles, roots, and soil of and around Pinus thunbergii naturally infected by PWN were investigated by using high-throughput sequencing coupled with the functional prediction (FUNGuild). The results showed that fungal richness, diversity, and evenness in the needles of diseased trees were significantly lower than those of healthy ones (p < 0.05), whereas no differences were found in the roots and soil. Principal coordinate analysis (PCoA) showed that the fungal community and functional structures significantly differed only in the needles of diseased and healthy trees, but not in the soil and roots. Functionally, the saprotrophs had a higher abundance in the needles of diseased trees, whereas symbiotrophs abundance was higher in the needles of healthy trees (linear discriminant analysis (LDA) > 2.0, p < 0.05). These results indicated that PWN infection primarily affected the fungal community and functional structures in the needles of P. thunbergii, but not the roots and soil.

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Evaluation of the novel endophytic fungus Chaetomium ascotrichoides 1‐24‐2 from Pinus massoniana as a biocontrol agent against pine wilt disease caused by Bursaphelenchus xylophilus

Kamaruzzaman,

Zheng,

Zhou

et al. 2024

Pest Management Science

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BACKGROUNDBursaphelenchus xylophilus, the causative agent of pine wilt disease (PWD), is an ever‐increasing threat to Pinus forests worldwide. This study aimed to develop biological control of PWD by the application of endophytic fungi isolated from healthy pine trees.RESULTSWe successfully isolated a novel endophytic fungal strain 1‐24‐2 from branches of healthy Pinus massoniana. The culture filtrates (CFs) of strain 1‐24‐2 exhibited strong nematicidal activity against Bursaphelenchus xylophilus, with a corrected mortality rate of 99.00%. Based on the morphological and molecular characteristics, the isolated strain 1‐24‐2 was identified as Chaetomium ascotrichoides. In the in‐planta assay, pine seedlings (2‐years‐old) treated with 1‐24‐2 CFs + pine wood nematode (T2) showed a significant control effect of 80%. A total of 24 toxic compounds were first identified from 1‐24‐2 CFs through gas chromatography–mass spectrometry (GC–MS) analysis, from which O‐methylisourea, 2‐chlorobenzothiazole, and 4,5,6‐trihydroxy‐7‐methylphthalide showed robust binding sites at Tyr119 against phosphoethanolamine methyltransferase (PMT) protein of Bursaphelenchus xylophilus by molecular docking approach and could be used as potential compounds for developing effective nematicides. Interestingly, strain 1‐24‐2 produces toxic volatile organic compounds (VOCs), which disturb the natural development process of B. xylophilus, whose total number decreased by up to 83.32% in the treatment group as compared to control and also reduced Botrytis cinerea growth by up to 71.01%.CONCLUSIONOur results highlight the potential of C. ascotrichoides 1‐24‐2 as a promising biocontrol agent with solid nematicidal activity against B. xylophilus. This is the first report of C. ascotrichoides isolated from P. massoniana exhibiting strong biocontrol potential against B. xylophilus in the world. © 2024 Society of Chemical Industry.

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Pinewood Nematode Alters the Endophytic and Rhizospheric Microbial Communities of Pinus massoniana

Cited by 22 publications

References 51 publications

Variations of Phyllosphere and Rhizosphere Microbial Communities of Pinus koraiensis Infected by Bursaphelenchus xylophilus

Variations of Phyllosphere and Rhizosphere Microbial Communities of Pinus koraiensis Infected by Bursaphelenchus xylophilus

The Impact of Pine Wood Nematode Infection on the Host Fungal Community

Evaluation of the novel endophytic fungus Chaetomium ascotrichoides 1‐24‐2 from Pinus massoniana as a biocontrol agent against pine wilt disease caused by Bursaphelenchus xylophilus

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