Pinus taeda phenylpropenal double-bond reductase: Purification, cDNA cloning, heterologous expression in Escherichia coli, and subcellular localization in P. taeda

Kasahara, Hiroyuki; Jin, Ying; Bedgar, Diana L.; Kim, Sungjin; Patten, Ann M.; Xia, Zhiqiang; Davin, Laurence B.; Lewis, Norman G.

doi:10.1016/j.phytochem.2006.07.001

Cited by 44 publications

(26 citation statements)

References 55 publications

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“…In conclusion, the localization of the dirigent protein mRNAs to the vascular cambium and radial parenchyma in actively growing stem tissue corresponds well with previous transcript localization of other lignan-related genes, such as that of pinoresinol-lariciresinol reductase in Forsythia intermedia and phenylcoumaran benzylic ether reductase in Pinus taeda , as well as the proposed phenylpropenal double-bond reductase in P. taeda (Kasahara et al, 2006). This pattern of expression is again consistent with ray parenchyma cells having an important role in heartwood formation as proposed by Chattaway (1952) and Hillis (1999).…”

Section: Discussionsupporting

confidence: 87%

Relationship of dirigent protein and 18s RNA transcript localization to heartwood formation in western red cedar

2008

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Section: Discussionsupporting

confidence: 87%

Relationship of dirigent protein and 18s RNA transcript localization to heartwood formation in western red cedar

2008

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“…Three genes encoding enzymes involved in biosynthesis of coniferyl alcohol-derived lignans were also found among the up-regulated genes in Cx, according to microarray analyses (Additional file 3). Pinoresinol-lariciresinol reductase (PLR: contig 44S, singletons BX251658 and BX252917) is an enzyme involved in the 8-8’ linked lignans pathway, and phenylcoumaran benzylic ether reductase (PCBER: contigs 17 and 17S, singleton FN256729) and phenylpropenal double-bond reductase (PPDBR: contig 36S, singleton BX255256) are involved in the 8-5’ linked lignans pathway [37]. The differential expression of genes encoding these enzymes was also validated in four trees by qRT-PCR, with higher transcript levels in Cx compared to Ox (Figure 8a).…”

Section: Resultsmentioning

confidence: 99%

Reprogramming of gene expression during compression wood formation in pine: Coordinated modulation of S-adenosylmethionine, lignin and lignan related genes

et al. 2012

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BackgroundTranscript profiling of differentiating secondary xylem has allowed us to draw a general picture of the genes involved in wood formation. However, our knowledge is still limited about the regulatory mechanisms that coordinate and modulate the different pathways providing substrates during xylogenesis. The development of compression wood in conifers constitutes an exceptional model for these studies. Although differential expression of a few genes in differentiating compression wood compared to normal or opposite wood has been reported, the broad range of features that distinguish this reaction wood suggest that the expression of a larger set of genes would be modified.ResultsBy combining the construction of different cDNA libraries with microarray analyses we have identified a total of 496 genes in maritime pine (Pinus pinaster, Ait.) that change in expression during differentiation of compression wood (331 up-regulated and 165 down-regulated compared to opposite wood). Samples from different provenances collected in different years and geographic locations were integrated into the analyses to mitigate the effects of multiple sources of variability. This strategy allowed us to define a group of genes that are consistently associated with compression wood formation. Correlating with the deposition of a thicker secondary cell wall that characterizes compression wood development, the expression of a number of genes involved in synthesis of cellulose, hemicellulose, lignin and lignans was up-regulated. Further analysis of a set of these genes involved in S-adenosylmethionine metabolism, ammonium recycling, and lignin and lignans biosynthesis showed changes in expression levels in parallel to the levels of lignin accumulation in cells undergoing xylogenesis in vivo and in vitro.ConclusionsThe comparative transcriptomic analysis reported here have revealed a broad spectrum of coordinated transcriptional modulation of genes involved in biosynthesis of different cell wall polymers associated with within-tree variations in pine wood structure and composition. In particular, we demonstrate the coordinated modulation at transcriptional level of a gene set involved in S-adenosylmethionine synthesis and ammonium assimilation with increased demand for coniferyl alcohol for lignin and lignan synthesis, enabling a better understanding of the metabolic requirements in cells undergoing lignification.

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“…Such moieties with a reduced side-chain had, however, been previously described in Pinus taeda (loblolly pine) cell suspension culture (171). Indeed, the allylic double bond reductases responsible for this reduction step, and the encoding genes have since been fully reported by our laboratory from both P. taeda (240,241) and Arabidopsis (242); the X-ray structure of the ternary complex has also been obtained and the proposed biochemical catalytic mechanism described (242). Moreover, the CAD loblolly pine mutant has been described as presumably harboring a number of mutations (74), with the plant line apparently stunted.…”

Section: Cad Mutant Loblolly Pine 2-methoxybenzaldehyde Andmentioning

confidence: 95%

Lignins: A Twenty‐First Century Challenge

Davin

Patten

Jourdes

et al. 2008

Biomass Recalcitrance

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Pinus taeda phenylpropenal double-bond reductase: Purification, cDNA cloning, heterologous expression in Escherichia coli, and subcellular localization in P. taeda

Cited by 44 publications

References 55 publications

Relationship of dirigent protein and 18s RNA transcript localization to heartwood formation in western red cedar

Relationship of dirigent protein and 18s RNA transcript localization to heartwood formation in western red cedar

Reprogramming of gene expression during compression wood formation in pine: Coordinated modulation of S-adenosylmethionine, lignin and lignan related genes

Lignins: A Twenty‐First Century Challenge

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