Piperine inhibits IL-β induced expression of inflammatory mediators in human osteoarthritis chondrocyte

Ying, Xiaozhou; Chen, Xiaowei; Cheng, Siyao; Shen, Yue; Lei, Peng; Xu, Hua

doi:10.1016/j.intimp.2013.06.025

Cited by 134 publications

(102 citation statements)

References 34 publications

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“…The mechanism of action of HA and CS seems to be complementary, developing a synergistic effect that could explain the results obtained in this study. Moreover, the addition of manganese, a trace element cofactor in the biosynthesis of glycosaminoglycan (20), and piperine, the active phenolic component of black pepper extract with its anti-inflammatory activity (21), may play a key role in the fast action of this compound.…”

Section: Discussionmentioning

confidence: 99%

Short-term effect of the combination of hyaluronic acid, chondroitin sulfate, and keratin matrix on early symptomatic knee osteoarthritis

Galluccio¹,

Barskova²,

Cerinic³

2015

Eur J Rheumatol

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Short-term effect of the combination of hyaluronic acid, chondroitin sulfate, and keratin matrix on early symptomatic knee osteoarthritis Abstract Objective: In the last years, symptomatic slow-acting drugs for osteoarthritis (SYSADOA) have been vastly studied and have generated considerable interest among clinicians. SYSADOA are generally used as a ground therapy with the main rationale to reduce the consumption of nonsteroidal anti-inflammatory drugs (NSAIDs) and thus limit the related adverse events. Material and Methods:In this study, we evaluated the short-term effect of an oral combination of hyaluronic acid, chondroitin sulfate, and keratin matrix on early symptomatic knee osteoarthritis. Forty patients were treated for 1 month and were allowed to assume analgesics or NSAIDs if necessary.Results: At 2 months, the mean reduction of the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score was 36% (p<0.001), and the mean reduction of the WOMAC pains score was 40% (p<0.001). Only two patients reported a sporadic need to assume analgesics; no patient reported any side effect during the study period.Conclusion: This data demonstrates that the oral combination of hyaluronic acid, chondroitin sulfate, and keratin matrix is safe, well tolerated, and shows a rapid action reducing pain and improving joint function and stiffness in early symptomatic knee osteoarthritis.

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Section: Discussionmentioning

confidence: 99%

Short-term effect of the combination of hyaluronic acid, chondroitin sulfate, and keratin matrix on early symptomatic knee osteoarthritis

Galluccio¹,

Barskova²,

Cerinic³

2015

Eur J Rheumatol

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“…IL-1β induces the expression of MMPs, such as MMP1, MMP3 and MMP13 that contribute to matrix degradation [23]. In addition, IL-1β induces the expression of iNOS and COX-2 and as well enhance the production of NO, PGE2 and IL-6 [11].…”

Section: Discussionmentioning

confidence: 99%

“…Astragalin, a bioactive component from Rosa agrestis [10] and piperine derived from Piper nigrum exhibited antiinflammatory effects in OA model [11]. In our study, we investigated the effects of nobiletin in IL-1β-induced OA.…”

Section: Osteoarthritismentioning

confidence: 99%

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Nobiletin Inhibits Expression of Inflammatory Mediators and Regulates JNK/ERK/p38 MAPK and PI3K/Akt Pathways in Human Osteoarthritic Chondrocytes

Liu¹,

Chong-wei²,

Wang³

et al. 2016

Trop. J. Pharm Res

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“…Full ethical consent was obtained from all patients. Cartilage was separated from underlying bone and connective tissues, and joint cartilage slices were isolated with hyaluronidase (Sigma) for five minutes, similar to previously published methods [23,24]. Chondrocytes were plated at 1×105 cells/ well in six well plate, serum starved overnight, pretreated with various concentrations of cordycepin (10, 50 or 100 μM) for two hours prior to IL-1β (5 ng/ml) for 24 hours.…”

Section: Chondrocyte Isolation and Culturesmentioning

confidence: 99%

Cordycepin prevented IL-β-induced expression of inflammatory mediators in human osteoarthritis chondrocytes

Ying

Lei

Chen

et al. 2013

International Orthopaedics (SICOT)

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Purpose Cordycepin, a nucleoside derivative isolated from Cordyceps, has been reported to exert anti-inflammatory, antitumor, antidiabetic and renoprotective effects. Osteoarthritis (OA) is a degenerative joint disease with an inflammatory component that drives the degradation of cartilage extracellular matrix. This study aimed to assess the effects of cordycepin on human OA chondrocytes. Methods In this study, human OA chondrocytes were pretreated with cordycepin at 10, 50 or 100 μM and subsequently stimulated with interleukin-1β (IL-1β) (5 ng/ml) for 24 h. Production of prostaglandin E 2 (PGE2) and nitric oxide (NO) were evaluated by the Griess reaction and an enzymelinked immunosorbent assay (ELISA). Gene expression of matrix metalloproteinase (MMP)-13, IL-6, inducible nitric oxide synthase (iNOS) and cyclo-oxygenase (COX-2) was measured by real-time polymerase chain reaction (PCR). MMP-13 and IL-6 proteins in culture medium were determined using cytokine-specific ELISA. Western immunoblotting was used to analyse the iNOS and COX-2 protein production in culture medium. Nuclear factor kappa-B (NF-κB) activity regulation was explored using Western immunoblotting. Results Pretreatment with cordycepin significantly inhibited the production of PGE2 and NO induced by IL-1β. Cordycepin also significantly decreased the IL-1β-stimulated gene expression and production of MMP-13, IL-6, iNOS and COX-2 in OA chondrocytes. Pretreatment with cordycepin attenuated IL-1β-induced activation of NF-κB by suppressing degradation of its inhibitory protein nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκB-α) in the cytoplasm. Conclusions We show for the first time the anti-inflammatory activity of cordycepin in human OA chondrocytes. Thus, with this unique profile of actions, cordycepin may prove to be a potentially attractive and new therapeutic/preventive agent for OA.

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Piperine inhibits IL-β induced expression of inflammatory mediators in human osteoarthritis chondrocyte

Cited by 134 publications

References 34 publications

Short-term effect of the combination of hyaluronic acid, chondroitin sulfate, and keratin matrix on early symptomatic knee osteoarthritis

Short-term effect of the combination of hyaluronic acid, chondroitin sulfate, and keratin matrix on early symptomatic knee osteoarthritis

Nobiletin Inhibits Expression of Inflammatory Mediators and Regulates JNK/ERK/p38 MAPK and PI3K/Akt Pathways in Human Osteoarthritic Chondrocytes

Cordycepin prevented IL-β-induced expression of inflammatory mediators in human osteoarthritis chondrocytes

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