Piperine inhibits LPS induced expression of inflammatory mediators in RAW 264.7 cells

Ying, Xiaozhou; Yu, Ke; Chen, Xiaowei; Chen, Hua; Jiang, Hong; Cheng, Siyao; Lei, Peng

doi:10.1016/j.cellimm.2013.09.001

Cited by 88 publications

(56 citation statements)

References 22 publications

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“…Recent studies have revealed that piperine exerts potent anti-inflammatory activity in mice experiencing endotoxin shock (Bae et al, 2010) and in rat models of arthritis (Bang et al, 2009;Kim and Lee, 2009;Umar et al, 2013). The ability of piperine to inhibit inflammation has been attributed to a reduction in inflammatory mediator expression by macrophages in the presence of piperine Ying et al, 2013). However, piperine also inhibits lipopolysaccharide-induced maturation of dendritic cells, suggesting a decreased capacity to present antigen to T lymphocytes (Bae et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Piperine blocks interleukin-2-driven cell cycle progression in CTLL-2 T lymphocytes by inhibiting multiple signal transduction pathways

et al. 2015

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Section: Introductionmentioning

confidence: 99%

Piperine blocks interleukin-2-driven cell cycle progression in CTLL-2 T lymphocytes by inhibiting multiple signal transduction pathways

et al. 2015

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“…The culture supernatant was assessed by Griess reaction as formerly performed [22]. The amount of nitrite in the samples was determined with reference to a sodium nitrite standard curve.…”

Section: Measurement Of No Tnf-α and Pge2mentioning

confidence: 99%

SeMet Mediates Anti-inflammation in LPS-Induced U937 Cells Targeting NF-κB Signaling Pathway

et al. 2014

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In previous studies, selenium (Se) was reported to play critical roles in anti-inflammatory activities. Nevertheless, limited information could be obtained during inflammation about selenomethionine (SeMet) in U937 human macrophage cells. The purpose of this study was to investigate the effects of SeMet on the inflammatory responses to lipopolysaccharide (LPS)-induced U937 macrophage cells and the signaling pathways targeted. U937 cells were pretreated with SeMet (1 μM) and subsequently induced with LPS (1 μg/ml) for 24 h. In the cell counting kit-8 assay (CCK-8), SeMet significantly inhibits the proliferation of U937 cells. SeMet also inhibited the production of nitric oxide (NO) and prostaglandin E2 (PGE2) stimulated by LPS. In the Western blot assay and real-time polymerase chain reaction (RT-PCR), SeMet significantly reduced protein expression and production of inducible NO synthase (iNOS), tumor necrosis factor-alpha (TNF-α), and COX-2 in U937 cells. Furthermore, SeMet markedly suppressed the LPS-mediated activation of nuclear factor-kappa B (NF-κB) by blocking the degradation of inhibitor-κB proteins (IκBα) and lessening the translocations of P50 subunit content of NF-κB in the nucleus. These findings suggested the anti-inflammatory activity of SeMet in U937 cells; indicating that SeMet might be a potential treatment for inflammation therapy.

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“…It has bioavailability enhancing activity for some drugs (Nirala et al, 2007(Nirala et al, , 2008. Piperine is known to exhibit a variety of biological activities including antiinflammatory (Ying et al, 2013), antioxidant (Jain & Mishra, 2011), antithyroid (Vijayakumar & Nalini, 2006), antimutagenic (Srinivasan, 2007), antitumor (Li et al, 2011), antidepressant (Mao et al, 2011), and hepatoprotective activity (Sahu et al, 2012). Curcumin is a hydrophobic polyphenol derived from the rhizome of the herb Curcuma longa has a wide spectrum of biological and pharmacological activities, including antioxidant (Sehgal et al, 2012), antiinflammatory (Abarikwu et al, 2014), antimicrobial and anticarcinogenic (Jagadeesh et al, 2009), hepatoprotective (Rajesh et al, 2010), and nephro-protective (Tarasub et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Co-administration of adjuvants along withMoringa oleiferaattenuates beryllium-induced oxidative stress and histopathological alterations in rats

Agrawal

Nirala

Shukla

et al. 2015

Pharmaceutical Biology

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Context: Moringa oleifera Lam. (Moringaceae) is a rich source of antioxidants. All parts of the plant are medicinally important and have been used as traditional medicine for a variety of human ailments in India. Objective: Therapeutic efficacy of adjuvants with M. oleifera (MO) root extract was investigated against beryllium-induced oxidative stress. Materials and methods: Hydroalcoholic (50% v/v) root extract of M. oleifera (150 mg/kg, p.o.) alone and combinations of M. oleifera with either piperine (2.5 mg/kg, p.o.) or curcumin (5.0 mg/kg, p.o.) daily for 1 week were administered in experimental rats against beryllium toxicity (1.0 mg/kg, i.p. daily for 5 weeks). Oxidative stress parameters including blood sugar, G-6-Pase in liver, and DNA damage were analyzed. Histopathological changes in liver and kidney were also observed. Results: Beryllium enhanced lipid peroxidation (LPO), depleted reduced glutathione (GSH) and antioxidant enzymes activities, decreased blood sugar and G-6-Pase activity, and did not damage DNA. Histologically, liver was observed with structural loss and disintegration of hepatocytes, heavy vacuolation in hepatocytes, and kidney was observed with constriction of glomeruli and hypertrophy in epithelial cells of uriniferous tubules. Therapy of M. oleifera with piperine was effective; however, combination of M. oleifera with curcumin showed better therapeutic effect by reduction of LPO, elevated GSH level, maintained antioxidant enzymes activities, restored blood sugar, and G-6-Pase activity in liver together with almost normal histoarchitecture of liver and kidney. Discussion and conclusion: Curcumin enhanced therapeutic efficacy of M. oleifera root extract and showed better antioxidant potential against beryllium toxicity.

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Piperine inhibits LPS induced expression of inflammatory mediators in RAW 264.7 cells

Cited by 88 publications

References 22 publications

Piperine blocks interleukin-2-driven cell cycle progression in CTLL-2 T lymphocytes by inhibiting multiple signal transduction pathways

Piperine blocks interleukin-2-driven cell cycle progression in CTLL-2 T lymphocytes by inhibiting multiple signal transduction pathways

SeMet Mediates Anti-inflammation in LPS-Induced U937 Cells Targeting NF-κB Signaling Pathway

Co-administration of adjuvants along withMoringa oleiferaattenuates beryllium-induced oxidative stress and histopathological alterations in rats

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