Context: Moringa oleifera Lam. (Moringaceae) is a rich source of antioxidants. All parts of the plant are medicinally important and have been used as traditional medicine for a variety of human ailments in India. Objective: Therapeutic efficacy of adjuvants with M. oleifera (MO) root extract was investigated against beryllium-induced oxidative stress. Materials and methods: Hydroalcoholic (50% v/v) root extract of M. oleifera (150 mg/kg, p.o.) alone and combinations of M. oleifera with either piperine (2.5 mg/kg, p.o.) or curcumin (5.0 mg/kg, p.o.) daily for 1 week were administered in experimental rats against beryllium toxicity (1.0 mg/kg, i.p. daily for 5 weeks). Oxidative stress parameters including blood sugar, G-6-Pase in liver, and DNA damage were analyzed. Histopathological changes in liver and kidney were also observed. Results: Beryllium enhanced lipid peroxidation (LPO), depleted reduced glutathione (GSH) and antioxidant enzymes activities, decreased blood sugar and G-6-Pase activity, and did not damage DNA. Histologically, liver was observed with structural loss and disintegration of hepatocytes, heavy vacuolation in hepatocytes, and kidney was observed with constriction of glomeruli and hypertrophy in epithelial cells of uriniferous tubules. Therapy of M. oleifera with piperine was effective; however, combination of M. oleifera with curcumin showed better therapeutic effect by reduction of LPO, elevated GSH level, maintained antioxidant enzymes activities, restored blood sugar, and G-6-Pase activity in liver together with almost normal histoarchitecture of liver and kidney. Discussion and conclusion: Curcumin enhanced therapeutic efficacy of M. oleifera root extract and showed better antioxidant potential against beryllium toxicity.

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M ycobacterium avium subspecies paratuberculosis (MAP) infects wide variety of animals and is endemic in the domestic livestock population of the country (Singh et al., 2010a). MAP has also been associated with Inflammatory Bowel disease (Crohn's disease) in human beings (Singh et al., 2008). Live cultivable MAP bacilli have been reported from milk based food items (ice creams, cheese, condensed milk,

Materials and methodsMilk sample collection from Local area in Farah and three different animals' cows, Goat and Buffalo with an average age were used for this research. Early morning fresh milk samples were collected from the Cow, Goat and Buffalo with separate containers using standard milking procedures. To avoid contamination, containers used for sample collection were sterilized by soaking in 10% HNO3 for 24hours; then in distilled water for another 24hours after which they were rinsed with more distilled water and finally dried ready for sample collection. The collected samples were immediately packaged and transported for analysis. Proximate AnalysisProximate parameters determined using the AOAC method. Moisture, ash, crude protein, and crude lipid contents were determined by oven drying, furnace, micro Kjeldah, soxlet extraction methods respectively [10]. Mineral analysisThe samples for mineral analysis were digested as per the procedure described by Kolmer [11] with slight modification. Briefly, 0.2 g sample was mixed with 7 ml of nitric acid (HNO3) and 1ml Hydrogen peroxide in 50 ml digestion tube. These samples were then kept in Rheological behavior and comparative analysis of nutrient composition of milk from three different breeds of goats, cows, and buffalo AbstractMilk is a considerable resource of products whose composition varies. Four components are dominant in quantitative terms: water, fat, protein and lactose; while the minor components are minerals, enzymes, vitamins, and dissolved gases. Exogenous nutrients supply in the human diet is required because of their biological significance for common life conservation. Milk is said to be the most unique and ideal class of food, because it meets the nutritional needs of the body better than any single food. This study to investigate the major constituents of milk obtained from three different breeds of cows, Goats and found in local area of village such as Farah reason. Proximate parameters -moisture, ash, crude protein and lipid were determined by AOAC methods and Rheological behavior such as viscosity analysis in milk sample and mineral element analysis by using ICPMS (Perkin Elmer) to determine calcium, phosphorus, sodium, potassium and magnesium respectively.

Carbosulfan is often used in agriculture for pest control on crops and for treatment against pyrethroid-resistant mosquitoes. This study investigated the impact of carbosulfan on oxidative stress markers, antioxidant defense, hematological, biochemical, and enzymological parameters in Sprague Dawley rats. Rats were orally administered carbosulfan doses of 1.02 to 10.20 mg/kg body weight daily; after 96 h, blood samples were taken, and the liver, kidney, and brain were dissected out for study. Results indicate that carbosulfan significantly increased the levels of lipid peroxidation and suppressed the activity of reduced glutathione, glutathione reductase, catalase, glucose-6-phosphate dehydrogenase, and adenosine triphosphatase. A mixed trend was observed in the activity of superoxide dismutase, while an increase was observed in the levels of serum uric acid, urea, aspartate aminotransferase, and alanine aminotransferase. Hemoglobin and albumin levels decreased but no significant differences were observed in creatinine and bilirubin levels. Future studies should include a more detailed analysis of the effects of chronic carbosulfan exposure on these biomarkers to further assess the impact of the pesticide on mammalian models.