piRNA-31115 Promotes Cell Proliferation and Invasion via PI3K/AKT Pathway in Clear Cell Renal Carcinoma

Du, Xinghua; Li, Haomin; Xie, Xuexia; Shi, Liping; Wu, Fan; Li, Guoliang; Lai, Caiyong; Heng, Baoli

doi:10.1155/2021/6915329

Cited by 11 publications

(6 citation statements)

References 22 publications

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“…However, none of the piRNA in our dataset mapped to LINE-1, suggesting another mechanism of action for these piRNA. This reasoning is supported by Du and colleagues who described the role of piR-31115 - also present in our dataset ( Supplementary File S2 )—in renal cell carcinoma progression by activation of epithelial-mesenchymal transition via the PI3K/AKT signaling, independently of L1 repression ( Du et al, 2021 ), as this piRNA also does not map to LINE-1.…”

Section: Discussionsupporting

confidence: 86%

Expression of Extracellular Vesicle PIWI-Interacting RNAs Throughout hiPSC-Cardiomyocyte Differentiation

et al. 2022

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Extracellular Vesicles (EV) play a critical role in the regulation of regenerative processes in wounded tissues by mediating cell-to-cell communication. Multiple RNA species have been identified in EV, although their function still lacks understanding. We previously characterized the miRNA content of EV secreted over hiPSC-cardiomyocyte differentiation and found a distinct miRNA expression in hiPSC-EV driving its in vitro bioactivity. In this work, we investigated the piRNA profiles of EV derived from key stages of the hiPSC-CM differentiation and maturation, i.e., from hiPSC (hiPSC-EV), cardiac progenitors (CPC-EV), immature (CMi-EV), and mature (CMm-EV) cardiomyocytes, demonstrating that EV-piRNA expression differs greatly from the miRNA profiles we previously identified. Only four piRNA were significantly deregulated in EV, one in hiPSC-EV, and three in CPC-EV, as determined by differential expression analysis on small RNA-seq data. Our results provide a valuable source of information for further studies aiming at defining the role of piRNA in the bioactivity and therapeutic potential of EV.

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Section: Discussionsupporting

confidence: 86%

Expression of Extracellular Vesicle PIWI-Interacting RNAs Throughout hiPSC-Cardiomyocyte Differentiation

et al. 2022

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“…DEGs among different m7G subtypes were enriched in cancer-related pathways, such as the positive regulation of cell adhesion, the positive regulation of vasculature development, cell molecule adhesion, the PI3K–Akt signaling pathway, and the AKT–STAT signaling pathway. PI3K–Akt signaling pathway activation can promote the metastasis and progression of RCC ( Du et al, 2021 ; Lin et al, 2021 ; Zhu et al, 2020 ). We divided the patients into three gene types in accordance with the three m7G subtypes and found significant differences in OS and PFS among the subtypes.…”

Section: Discussionmentioning

confidence: 99%

m7G regulator-mediated molecular subtypes and tumor microenvironment in kidney renal clear cell carcinoma

Chen

Nie²,

Gao³

et al. 2022

Front. Pharmacol.

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Background: RNA methylation modification plays an important role in immune regulation. m7G RNA methylation is an emerging research hotspot in the RNA methylation field. However, its role in the tumor immune microenvironment of kidney renal clear cell carcinoma (KIRC) is still unclear.Methods: We analyzed the expression profiles of 29 m7G regulators in KIRC, integrated multiple datasets to identify a novel m7G regulator-mediated molecular subtype, and developed the m7G score. We evaluated the immune tumor microenvironments in m7G clusters and analyzed the correlation of the m7G score with immune cells and drug sensitivity. We tested the predictive power of the m7G score for prognosis of patients with KIRC and verified the predictive accuracy of the m7G score by using the GSE40912 and E-MTAB-1980 datasets. The genes used to develop the m7G score were verified by qRT-PCR. Finally, we experimentally analyzed the effects of WDR4 knockdown on KIRC proliferation, migration, invasion, and drug sensitivity.Results: We identified three m7G clusters. The expression of m7G regulators was higher in cluster C than in other clusters. m7G cluster C was related to immune activation, low tumor purity, good prognosis, and low m7G score. Cluster B was related to drug metabolism, high tumor purity, poor survival, and high m7G score. Cluster A was related to purine metabolism. The m7G score can well-predict the prognosis of patients with KIRC, and its prediction accuracy based on the m7G score nomogram was very high. Patients with high m7G scores were more sensitive to rapamycin, gefitinib, sunitinib, and vinblastine than other patients. Knocking down WDR4 can inhibit the proliferation, migration, and invasion of 786-0 and Caki-1 cells and increase sensitivity to sorafenib and sunitinib.Conclusion: We proposed a novel molecular subtype related to m7G modification and revealed the immune cell infiltration characteristics of different subtypes. The developed m7G score can well-predict the prognosis of patients with KIRC, and our research provides a basis for personalized treatment of patients with KIRC.

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“…It has been demonstrated that piR-823 was significantly upregulated in the ALDH-positive breast CSCs, and piR-823 confers stem-like properties to breast cancer cells by activating the Wnt signaling pathway (Ding et al, 2021). In clear cell renal carcinoma cells, piR-31115 induces epithelialmesenchymal transition (EMT) via decreasing E-cadherin expression and increasing mesenchymal markers (Vimentin and Snail) (Du et al, 2021). These results suggest that the expression of certain piRNAs is required for the initiation and maintenance of CSCs, and the roles of piRNAs in gastrointestinal CSCs deserve further investigation.…”

Section: Therapeutic Approaches Using Piwi-interacting Rnasmentioning

confidence: 99%

PIWI-Interacting RNAs (piRNAs): Promising Applications as Emerging Biomarkers for Digestive System Cancer

Cai

Zhou

et al. 2022

Front. Mol. Biosci.

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PIWI-interacting RNAs (piRNAs) are a novel type of small non-coding RNAs (sncRNAs), which are 26–31 nucleotides in length and bind to PIWI proteins. Although piRNAs were originally discovered in germline cells and are thought to be essential regulators for germline preservation, they can also influence gene expression in somatic cells. An increasing amount of data has shown that the dysregulation of piRNAs can both promote and repress the emergence and progression of human cancers through DNA methylation, transcriptional silencing, mRNA turnover, and translational control. Digestive cancers are currently a major cause of cancer deaths worldwide. piRNAs control the expression of essential genes and pathways associated with digestive cancer progression and have been reported as possible biomarkers for the diagnosis and treatment of digestive cancer. Here, we highlight recent advances in understanding the involvement of piRNAs, as well as potential diagnostic and therapeutic applications of piRNAs in various digestive cancers.

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piRNA-31115 Promotes Cell Proliferation and Invasion via PI3K/AKT Pathway in Clear Cell Renal Carcinoma

Cited by 11 publications

References 22 publications

Expression of Extracellular Vesicle PIWI-Interacting RNAs Throughout hiPSC-Cardiomyocyte Differentiation

Expression of Extracellular Vesicle PIWI-Interacting RNAs Throughout hiPSC-Cardiomyocyte Differentiation

m7G regulator-mediated molecular subtypes and tumor microenvironment in kidney renal clear cell carcinoma

PIWI-Interacting RNAs (piRNAs): Promising Applications as Emerging Biomarkers for Digestive System Cancer

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