Pisatin metabolism in pea (Pisum sativum L.) cell suspension cultures

Abstract

ABSTRACT. Cell suspension cultures were established from germinating pea (Pisum sativum L.) seeds. This cell culture, which accumulated pisatin, consisted mostly of single cells containing a few cell aggregates. The cells responded to treatment with a yeast glucan preparation with transient accumulation of pisatin in both cells and culture media. Addition of pisatin to cell cultures resulted in increased synthesis of pisatin. Phenylalanine ammonia-lyase, chalcone synthase and isoflavone reductase activities were present in untreated cells. Upon treatment with an elicitor preparation the activities of the first two enzymes showed a rapid, transient increase up to 20 hours after treatment. Isoflavone reductase showed a major and minor peak at 16 and 36 h, respectively, after elicitor treatment. The time course of the enzyme activity and pisatin accumulation is consistent with an elicitormediated response.

Biotechnology approaches to overcome biotic and abiotic stress constraints in legumes

“…Most studies on plant stress responses focused on flavonoid and isoflavonoid phytoalexins. Accumulation of medicarpin, pisatin, glyceolin or sativan has been frequently observed in response to pathogen infection and elicitor treatment in alfalfa, pea, soybean and L. japonicus respectively (Baldridge and Samac, 1998;BorejszaWysocki et al, 1997;Lozovaya et al, 2004;Saunders & O'Neill, 2004;Shimada et al, 2000). Interestingly, phytoalexin accumulation was also observed after copper or mercury stress (Mithofer et al, 2004).…”

Alterations in Taxol Production in Plant Cell Culture via Manipulation of the Phenylalanine Ammonia Lyase Pathway

“…Extract preparation followed the procedure as described (31), except that sodium borate, 0.05 M, pH 8.9 was used, and 2‐mercaptoethanol at a concentration of 6 mM and 1 mM PMSF were added to the buffer on the day of the experiment. Use of borate buffer enabled detection of PAL activity at levels comparable to that reported in bean cell suspension (31). Cell extraction was carried out at 4 °C using 0.1 g of cells homogenized in 0.4 mL of chilled buffer as described above.…”