Pisatin Production by Tissue Cultures of Pisum sativum L.

Bailey, J. A.

doi:10.1099/00221287-61-3-409

Cited by 47 publications

(9 citation statements)

References 6 publications

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“…Pisatin content of the untreated cell suspension increased approximately ten fold of the original value in a 72 hour period, and showed a marked decline thereafter. A similar observation was made with pea callus (Bailey, 1970). When a pea cell suspension was exposed to the elicitor (one mg glucose equivalent per 100 ml medium), the cell cultures responded with an increased production of pisatin (Fig.…”

Section: Accumulation and Metabolism Of Pisatinsupporting

confidence: 77%

Pisatin metabolism in pea (Pisum sativum L.) cell suspension cultures

1997

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ABSTRACT. Cell suspension cultures were established from germinating pea (Pisum sativum L.) seeds. This cell culture, which accumulated pisatin, consisted mostly of single cells containing a few cell aggregates. The cells responded to treatment with a yeast glucan preparation with transient accumulation of pisatin in both cells and culture media. Addition of pisatin to cell cultures resulted in increased synthesis of pisatin. Phenylalanine ammonia-lyase, chalcone synthase and isoflavone reductase activities were present in untreated cells. Upon treatment with an elicitor preparation the activities of the first two enzymes showed a rapid, transient increase up to 20 hours after treatment. Isoflavone reductase showed a major and minor peak at 16 and 36 h, respectively, after elicitor treatment. The time course of the enzyme activity and pisatin accumulation is consistent with an elicitormediated response.

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Section: Accumulation and Metabolism Of Pisatinsupporting

confidence: 77%

Pisatin metabolism in pea (Pisum sativum L.) cell suspension cultures

1997

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“…sesamin and kobusin), and alkaloids (e.g. sanguinarine, berberin, aristolochic acid, and narciclasine; Bailey, 1970;Trifunovic et al, 2003;Ono et al, 2006;Evidente et al, 1983;Gardiner et al, 2008;Na et al, 2011;Hu et al, 2012;Nakagawa et al, 2012;Hara and Kurita, 2014). Interestingly, the bioactivity of berberin and coptisin has been linked to the dioxole group as a structural analog, but without this functional group (i.e.…”

Section: Discussionmentioning

confidence: 99%

The allelochemical MDCA inhibits lignification and affects auxin homeostasis

et al. 2016

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The phenylpropanoid 3,4-(methylenedioxy)cinnamic acid (MDCA) is a plant-derived compound first extracted from roots of Asparagus officinalis and further characterized as an allelochemical. Later on, MDCA was identified as an efficient inhibitor of 4-COUMARATE-CoA LIGASE (4CL), a key enzyme of the general phenylpropanoid pathway. By blocking 4CL, MDCA affects the biosynthesis of many important metabolites, which might explain its phytotoxicity. To decipher the molecular basis of the allelochemical activity of MDCA, we evaluated the effect of this compound on Arabidopsis thaliana seedlings. Metabolic profiling revealed that MDCA is converted in planta into piperonylic acid (PA), an inhibitor of CINNAMATE-4-HYDROXYLASE (C4H), the enzyme directly upstream of 4CL. The inhibition of C4H was also reflected in the phenolic profile of MDCA-treated plants. Treatment of in vitro grown plants resulted in an inhibition of primary root growth and a proliferation of lateral and adventitious roots. These observed growth defects were not the consequence of lignin perturbation, but rather the result of disturbing auxin homeostasis. Based on DII-VENUS quantification and direct measurement of cellular auxin transport, we concluded that MDCA disturbs auxin gradients by interfering with auxin efflux. In addition, mass spectrometry was used to show that MDCA triggers auxin biosynthesis, conjugation, and catabolism. A similar shift in auxin homeostasis was found in the c4h mutant ref3-2, indicating that MDCA triggers a cross talk between the phenylpropanoid and auxin biosynthetic pathways independent from the observed auxin efflux inhibition. Altogether, our data provide, to our knowledge, a novel molecular explanation for the phytotoxic properties of MDCA.

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“…Reported abiotic elicitors for phytoalexin accumulation include heavy metals, especially mercuric and cupric chloride (Hargreaves 1979), respiratory inhibitors (Cheema and Haard 1978), detergents and other chemicals (Bailey 1982), mechanical wounding (Yoshikawa 1978), partial freezing (Lyon and Albersheim 1982), and UV light (Hadwiger and Schwochau 1971). Davis et al (1986) reported that biotic and abiotic elicitors can act synergistically in stimulating phytoalexin accumulation.…”

Section: Discussionmentioning

confidence: 99%

Mansonone accumulation in elm callus induced by elicitors of Ophiostoma ulmi, and general properties of elicitors

Yang¹,

Jeng²,

Hubbes³

1989

Can. J. Bot.

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Fungal culture filtrates, cytoplasm, and cell walls of Ophiostoma ulmi contain molecules that elicit mansonone (phytoalexin) accumulation in elm calli. The elicitors from nonaggressive isolate Q311 cause a more rapid reaction on bioassays than those from the aggressive isolate MH75. On the callus of a susceptible elm (Ulmus americana), the elicitors from both isolates cause higher amounts of mansonone production than on the callus of a resistant elm (Ulmus pumila). These elicitors are heat stable, and their eliciting activities are partially sensitive to NaIO4, pronase E, proteinase K, and various hydrolytic enzymes. However, β-glucosidase treated elicitors have increased eliciting activities. The binding of Q311 culture filtrate elicitors to a concanavalin A – sepharose 4B column suggests that glycoproteins may be involved in elicitation. Some abiotic factors stimulate only small amounts of mansonone F accumulation in treated elm cells.

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Pisatin Production by Tissue Cultures of Pisum sativum L.

Cited by 47 publications

References 6 publications

Pisatin metabolism in pea (Pisum sativum L.) cell suspension cultures

Pisatin metabolism in pea (Pisum sativum L.) cell suspension cultures

The allelochemical MDCA inhibits lignification and affects auxin homeostasis

Mansonone accumulation in elm callus induced by elicitors of Ophiostoma ulmi, and general properties of elicitors

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