Pisum sativum seed globulins as biochemical markers for « wrinkled-smooth » seed character: comparison of genotypes through different protein extraction and electrophoretic procedures

Rao, R.; Pernollet, Jean‐Claude

doi:10.1051/agro:19811012

Cited by 10 publications

(5 citation statements)

References 4 publications

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“…The presence or absence of particular bands or groups of bands in association with particular characteristics or traits might indicate the involvement of the polypeptide subunit in the development, or otherwise, of the trait. Rao and Pernolett (1981) found correlation between wrinkled phenotypes and the presence of a 60 kDa vicihn band in Pisum sativum. In a previous study (Odeigah and Osanyinpeju 1996), we observed the presence of two polypeptide bands (39 and 20 kDa) to be associated with insect pest resistance (including aphids) in cultivars of cowpea.…”

Section: Discussionmentioning

confidence: 85%

“…Thomson and Schroeder (1978) showed that the vicilin variants are controlled by alternative alleles at single loci. Rao and Pernolett (1981) explained that the complexity could result from duplication(s) of original genes, with subsequent independent development. Duplication(s) of genes or parts of genes appears to have occurred frequently during seed storage-protein evolution (Thomson and Doll 1979).…”

Section: Discussionmentioning

confidence: 99%

“…The total seed protein, globulin and albumin fractions were obtained according to the methods of Khan et al (1980) and Rao and Pernolett (1981) as described previously (Odeigah and Osanyinpeju 1996). Briefly, the defatted meals were extracted with 0.05 M Tris-glycine buffer and recovered by centrifugation.…”

Section: Methodsmentioning

confidence: 99%

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Variation in seed proteins from mutagen‐treated cultivars and selected lines of Vigna unguiculata L. Walp

Osanyinpeju

Odeigah

1998

Plant Breeding

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The storage protein profiles of the seeds of two IITA cowpea cultivars ('IT84E-124' and 'Vita 7') exposed to three mutagens -sodium azide (NaN3), ethyl methane sulphonate (EMS) and ^''Co gamma rays -and those of 18 selected M3 lines were investigated by sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE). The total seed protein, globulin and albumin fractions showed differences in number and intensity of subunit bands, but the least differences were found in the albumin fraction. Both high and low molecular weight bands were observed, the highest being 94.5 kDa and the lowest 12.0 kDa. 'Vita T showed less variability compared with 'IT84E-124', as indicated by the relative similarity indices (S.I.) ofthe two cultivars. The lowest S.I. of 0.200 was found among 'IT84E-124' lines while the lowest S.I. among 'Vita T lines was 0.4706. A number of lines with particular traits were found to be characterized by the presence of specific polypeptide bands. This study demonstrates that induced mutation could create additional variability to supplement existing germplasm and that SDS-PAGE is a useful tool for discriminating and estimating genetic similarities among selections.

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

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Variation in seed proteins from mutagen‐treated cultivars and selected lines of Vigna unguiculata L. Walp

Osanyinpeju

Odeigah

1998

Plant Breeding

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“…Differences in the presence or absence of bands among accessions are indicative of differences in the genes controlling the different polypeptide subunits (Osanyinpeju and Odeigah, 1998). Also, the presence or absence of polypeptide bands has been found to be linked to the expression of certain characteristics such as wrinkled seeds and insect resistance (Rao and Pernolett, 1981;Odeigah and Osanyinpeju, 1996). The seed shape of NH/2016/P04 was observed to be wrinkled and the absence of the 24 kDa band in this accession may be responsible for this seed texture as it was the only accession expressing this characteristic.…”

Section: Seed Storage Protein Analysismentioning

confidence: 99%

Biodiversity assessment of African locust bean (Parkia biglobosa) accessions from Savanna and Forest zones of Nigeria as revealed by seed storage proteins and RAPD markers

Akin-Idowu¹,

Aduloju²,

Akinyoola³

et al. 2021

GenResJ

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Understanding the level and distribution of genetic diversity in African locust bean (Parkia biglobosa) would strengthen breeding and conservation programmes towards domestication and sustainable use of this species. Sixteen accessions of P. globosa were assessed for variability based on seed morphology, seed protein and DNA profiling. Significant variation in seed characteristics were observed across locations. Seed protein profiling by SDS-PAGE revealed homogeneity as most bands were found common in all accessions, indicating that the protein profiles are highly conserved. Protein profiling separated the 16 accessions into four major clusters at 0.93 similarity coefficient. Most accessions grouping into Cluster 1 had a similarity coefficient of close to 100% and were from the Derived Savanna suggesting the presence of duplicates. Accessions NH/2016/P14, NH/2016/P03 and NH/2016/P04 grouped into clusters II, III and IV; respectively. Sixteen RAPD markers generated a total of 256 bands of which 63.67% were polymorphic. Gene diversity ranged from 0.41 to 0.93 and Polymorphic Information Content (PIC) from 0.39 to 0.93. The RAPD-based dendrogram separated accessions into six groups at 0.68 similarity coefficient. Based on a polymorphic seed storage protein marker a genetically distinct accession NH/2016/P04 could be exploited for breeding purposes. The homogeneity of alleles and narrow genetic base as revealed by RAPD and SDS-PAGE analyses suggests possible loss of intraspecific genetic diversity. Thus, intensification of germplasm collections across the different agroecological zones and characterization using specific markers will give a better understanding of diversity of P. biglobosa in order to enhance selection towards conservation, breeding and sustainable utilization.

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“…The crude extract was extensively dialyzed (cut-off MW 12 000) against distilled water and centrifuged to provide water-soluble (albumin) and -insoluble (globulin) fractions. 18 Both fractions were lyophilized and used for further analyses.…”

Section: Preparation Of the Albumin And Globulin Fractionsmentioning

confidence: 99%

In vitro and in vivo digestibility of the albumin and globulin fractions of eight Brazilian cowpea [Vigna unguiculata (L) Walp] cultivars

et al. 2004

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Cowpea (Vigna unguiculata) is a very important crop for people living in the north-east of Brazilwhere it constitutes the principal source of protein and carbohydrate. In this study, it was observed that the in vitro digestibility of the seed albumin and globulin fractions extracted from eight Brazilian cowpea cultivars was high when treated with pepsin but resistant to degradation by trypsin or chymotrypsin even after 3 h of treatment. When the albumin and globulin fractions were given to rats by intragastric intubation it was observed that intact proteins or their fragments were immunodetected in the faeces. Immunohistochemical analyses of the gut sections of these rats showed that a proportion of the albumin fraction (or its fragments), but not the globulin one, was bound to the brush border enterocytes in the duodenum. Thus the incomplete enzymatic degradation of both globulin and albumin fractions as well as the albumin binding to the gut may have a bearing upon the growth depression, malabsorption and diarrhoea observed on rats fed on cowpea seeds.

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Pisum sativum seed globulins as biochemical markers for « wrinkled-smooth » seed character: comparison of genotypes through different protein extraction and electrophoretic procedures

Cited by 10 publications

References 4 publications

Variation in seed proteins from mutagen‐treated cultivars and selected lines of Vigna unguiculata L. Walp

Variation in seed proteins from mutagen‐treated cultivars and selected lines of Vigna unguiculata L. Walp

Biodiversity assessment of African locust bean (Parkia biglobosa) accessions from Savanna and Forest zones of Nigeria as revealed by seed storage proteins and RAPD markers

In vitro and in vivo digestibility of the albumin and globulin fractions of eight Brazilian cowpea [Vigna unguiculata (L) Walp] cultivars

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