Over time, a bird population's acoustic and morphological features can diverge from the parent species. A quantitative measure of difference between two populations of species/subspecies is extremely useful to zoologists. Work in this paper takes a dialect difference system first developed for speech and refines it to automatically measure vocalisation difference between bird populations by extracting pitch contours. The pitch contours are transposed into pitch codes. A variety of codebook schemes are proposed to represent the contour structure, including a vector quantization approach. The measure, called Bird Vocalisation Difference, is applied to bird populations with calls that are considered very similar, very different, and between these two extremes. Initial results are very promising, with the behaviour of the metric consistent with accepted levels of similarity for the populations tested to date. The influence of data size on the measure is investigated by using reduced datasets. Results of species pair classification using Gaussian mixture models with Mel-frequency cepstral coefficients is also given as a baseline indicator of class confusability.