Pivoting of microtubules driven by minus end directed motors leads to their alignment to form an interpolar bundle

Winters, Lora; Ban, Ivana; Prelogović, Marcel; Pavin, Nenad; Tolić, Iva M.

doi:10.1101/347831

Cited by 4 publications

(4 citation statements)

References 81 publications

(101 reference statements)

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“…In these simulations, antiparallel cross-linking by Ase1 and the stabilization of cross-linked MT dynamics are sufficient for model bipolar spindle assembly in the absence of motors (51). Related previous theory has studied mitotic MT bundling by motors and cross-linkers (73,74).…”

Section: Introductionmentioning

confidence: 89%

Theory of Cytoskeletal Reorganization during Cross-Linker-Mediated Mitotic Spindle Assembly

Lamson

Edelmaier

Glaser

et al. 2019

Biophysical Journal

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Cells grow, move, and respond to outside stimuli by large-scale cytoskeletal reorganization. A prototypical example of cytoskeletal remodeling is mitotic spindle assembly, during which microtubules nucleate, undergo dynamic instability, bundle, and organize into a bipolar spindle. Key mechanisms of this process include regulated filament polymerization, cross-linking, and motor-protein activity. Remarkably, using passive cross-linkers, fission yeast can assemble a bipolar spindle in the absence of motor proteins. We develop a torque-balance model that describes this reorganization because of dynamic microtubule bundles, spindle-pole bodies, the nuclear envelope, and passive cross-linkers to predict spindle-assembly dynamics. We compare these results to those obtained with kinetic Monte Carlo-Brownian dynamics simulations, which include cross-linker-binding kinetics and other stochastic effects. Our results show that rapid cross-linker reorganization to microtubule overlaps facilitates cross-linker-driven spindle assembly, a testable prediction for future experiments. Combining these two modeling techniques, we illustrate a general method for studying cytoskeletal network reorganization.

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Section: Introductionmentioning

confidence: 89%

Theory of Cytoskeletal Reorganization during Cross-Linker-Mediated Mitotic Spindle Assembly

Lamson

Edelmaier

Glaser

et al. 2019

Biophysical Journal

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show abstract

“…In these simulations, antiparallel crosslinking by Ase1 and the stabilization of crosslinked MT dynamics are sufficient for model bipolar spindle assembly in the absence of motors [51]. Related previous theory has studied mitotic MT bundling by motors and crosslinkers [73,74].…”

Section: Introductionmentioning

confidence: 87%

Theory of cytoskeletal reorganization during crosslinker-mediated mitotic spindle assembly

Lamson¹,

Edelmaier²,

Glaser³

et al. 2018

Preprint

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Cells grow, move, and respond to outside stimuli by large-scale cytoskeletal reorganization. A prototypical example of cytoskeletal remodeling is mitotic spindle assembly, during which microtubules nucleate, undergo dynamic instability, bundle, and organize into a bipolar spindle. Key mechanisms of this process include regulated filament polymerization, crosslinking, and motor-protein activity. Remarkably, using passive crosslinkers, fission yeast can assemble a bipolar spindle in the absence of motor proteins. We develop a torque-balance model that describes this reorganization due to dynamic microtubule bundles, spindle-pole bodies, the nuclear envelope, and passive crosslinkers to predict spindle-assembly dynamics. We compare these results to those obtained with kinetic Monte Carlo-Brownian dynamics simulations, which include crosslinker-binding kinetics and other stochastic effects. Our results show that rapid crosslinker reorganization to microtubule overlaps facilitates crosslinker-driven spindle assembly, a testable prediction for future experiments. Combining these two modeling techniques, we illustrate a general method for studying cytoskeletal network reorganization.

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“…The motor model described here for two dimensions is analogous to the phenomenological model for one dimension described in Kruse and Jülicher (2000); these one-dimensional calculations show that the relative velocity between two antialigned MTs is a linear function of pa and km. Similarly, a kinesin-5 induced effective torque between MTs has been calculated to study forces in the mitotic spindle (Winters et al, 2018).…”

Section: Introductionmentioning

confidence: 93%

Chronology of motor-mediated microtubule streaming

Ravichandran

Duman

Hoore

et al. 2019

eLife

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We introduce a filament-based simulation model for coarse-grained, effective motor-mediated interaction between microtubule pairs to study the time-scales that compose cytoplasmic streaming. We characterise microtubule dynamics in two-dimensional systems by chronologically arranging five distinct processes of varying duration that make up streaming, from microtubule pairs to collective dynamics. The structures found were polarity sorted due to the propulsion of antialigned microtubules. This also gave rise to the formation of large polar-aligned domains, and streaming at the domain boundaries. Correlation functions, mean squared displacements, and velocity distributions reveal a cascade of processes ultimately leading to microtubule streaming and advection, spanning multiple microtubule lengths. The characteristic times for the processes extend over three orders of magnitude from fast single-microtubule processes to slow collective processes. Our approach can be used to directly test the importance of molecular components, such as motors and crosslinking proteins between microtubules, on the collective dynamics at cellular scale.

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Pivoting of microtubules driven by minus end directed motors leads to their alignment to form an interpolar bundle

Cited by 4 publications

References 81 publications

Theory of Cytoskeletal Reorganization during Cross-Linker-Mediated Mitotic Spindle Assembly

Theory of Cytoskeletal Reorganization during Cross-Linker-Mediated Mitotic Spindle Assembly

Theory of cytoskeletal reorganization during crosslinker-mediated mitotic spindle assembly

Chronology of motor-mediated microtubule streaming

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