PKA-Bad-14-3-3 and Akt-Bad-14-3-3 signaling pathways are involved in the protective effects of PACAP against ischemia/reperfusion-induced cardiomyocyte apoptosis

Rácz, Bóglárka; Gasz, Balázs; Gallyas, Ferenc; Kiss, P.; Tamás, Andrea; Szántó, Zalán; Lubics, Andrea; Lengvári, Istvàn; Tóth, Gábor; Hegyi, Orsolya; Röth, Erzsébet; Reglődi, Dóra

doi:10.1016/j.regpep.2007.09.015

Cited by 57 publications

(54 citation statements)

References 59 publications

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“…[29][30][31] In the present study, the number of TUNEL positive nuclei increased in PACAP+/-mice after Dox injection ( Figure 5C). This data indicates endogenous PACAP has anti-apoptotic effects in cardiac tissue.…”

Section: Pacap and Heart Diseasesupporting

confidence: 62%

Cardioprotective Effect of Endogenous Pituitary Adenylate Cyclase-Activating Polypeptide on Doxorubicin-Induced Cardiomyopathy in Mice

Mori

Nakamachi

Ohtaki

et al. 2010

Circ J

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Circulation Journal Official Journal of the Japanese Circulation Society http://www. j-circ.or.jp ituitary adenylate cyclase-activating polypeptide (PACAP) is a regulatory peptide that was first isolated from the ovine hypothalamus. 1 PACAP exists in 2 amidated forms of either 38 and 27 residues, designated PACAP38 and PACAP27, respectively. 2 PACAP is a member of the vasoactive intestinal polypeptide (VIP)/secretin/ growth hormone releasing hormone family of peptides, and its amino acid sequence shows great similarity to VIP.PACAP and VIP share 3 kinds of receptors, namely PAC1-receptor (PAC1R), and the VPAC1 and VPAC2 receptors. The affinity of PAC1R with PACAP is 100-1,000 times higher than with VIP, indicating that PAC1R is a relatively selective receptor for PACAP. 3 PACAP is widely distributed in the brain and peripheral organs in mammals, and reportedly has diverse functions in the endocrine, nervous, gastrointestinal, immune, and cardiovascular systems. 4,5 PACAP and its receptors have also been identified in mammalian heart. 6-11 PACAP modulates the excitability of intracardiac neurons 6,12,13 and has positive inotropic, chronotropic, and dromotoropic effects on cardiomyocyte. 14-16 PACAP is also a potent vasodilator in various organs, including coronary and pulmonary arteries. PACAP and its receptors are expressed in the heart, but it is unclear whether PACAP exerts its protective effect on the myocardium in vivo. The aim of the present study was to investigate whether endogenous PACAP has a cardioprotective effect on Doxorubicin (Dox)-induced cardiomyopathy.

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Section: Pacap and Heart Diseasesupporting

confidence: 62%

Cardioprotective Effect of Endogenous Pituitary Adenylate Cyclase-Activating Polypeptide on Doxorubicin-Induced Cardiomyopathy in Mice

Mori

Nakamachi

Ohtaki

et al. 2010

Circ J

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“…antagonists 41,42 (VIP ; PACAP (Bachem) were applied to cells in a 100-fold concentration 3 hours before neuropeptide was added, following the procedure of Rácz et al 43 Experiments were performed four times (two double experiments). IBMX (3-isobutyl-1-methylxanthin; Sigma; 4 mmol/L) was used to measure total intracellular cAMP due to receptor stimulation.…”

Section: Cell Culture and Camp Eia-assaymentioning

confidence: 99%

Pituitary Adenylate Cyclase Activating Polypeptide

Seeliger

Buddenkotte

Schmidt‐Choudhury

et al. 2010

The American Journal of Pathology

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“…PACAP also exhibits protective properties against oxidative stress-induced apoptosis in cardiomyocytes (Gasz et al, 2006;Rá cz et al, 2008;Roth et al, 2009) and endothelial cells (Rá cz et al, 2007), pointing to the therapeutic interest of PACAP for the treatment of cardiovascular diseases.…”

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confidence: 99%

Pituitary Adenylate Cyclase-Activating Polypeptide and Its Receptors: 20 Years after the Discovery

et al. 2009

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PKA-Bad-14-3-3 and Akt-Bad-14-3-3 signaling pathways are involved in the protective effects of PACAP against ischemia/reperfusion-induced cardiomyocyte apoptosis

Cited by 57 publications

References 59 publications

Cardioprotective Effect of Endogenous Pituitary Adenylate Cyclase-Activating Polypeptide on Doxorubicin-Induced Cardiomyopathy in Mice

Cardioprotective Effect of Endogenous Pituitary Adenylate Cyclase-Activating Polypeptide on Doxorubicin-Induced Cardiomyopathy in Mice

Pituitary Adenylate Cyclase Activating Polypeptide

Pituitary Adenylate Cyclase-Activating Polypeptide and Its Receptors: 20 Years after the Discovery

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